FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062643905> ?p ?o ?g. }

• W2062643905 endingPage "4843" @default.
• W2062643905 startingPage "4834" @default.
• W2062643905 abstract "Myosin subfragment 1 (S1) is the ATP catalyzing motor protein in muscle. It consists of three domains that catalyze ATP and bind actin (catalytic), conduct energy transduction (converter), and transport the load (lever arm). These domains interface in two places identified as interface I, containing the reactive thiol (SH1) and ATP-sensitive tryptophan (Trp510), and interface II, containing the reactive lysine residue (RLR). Two crystal structures of S1 were extrapolated to working in solution or oriented in tissue forms, using structure-sensitive optical spectroscopic signals from extrinsic probes located in the interfaces. Observed signals included circular dichroism (CD) and absorption originating from S1 in solution in the presence and absence of actin and fluorescence polarization from cross-bridges in muscle fibers. Theoretical signals were calculated from S1 crystal structure models perturbed with lever arm movement from swiveling at three conserved glycines, 699, 703, and 710 (chicken skeletal myosin numbering). Structures giving the best agreement between the computed and observed signals were selected as the representative forms. Both interfaces undergo dramatic conformational change during ATPase and force development. Changes at interface I suggest the molecular basis for the collisional quenching sensitivity of Trp510 to nucleotide binding. The probe conformation at SH1 suggests how it alters S1 ATPases. At interface II, the spatial relationship of the lever arm and the extrinsic probe at RLR suggests how the probe alters S1 ATPases and that it should inhibit lever arm movement during the power stroke. The latter possibility, if true, establishes a part of the corridor through which the lever arm swings during the power stroke. Global structural changes in actomyosin are discussed in the accompanying paper [Burghardt et al. (2001) Biochemistry 40, 4821-4833]." @default.
• W2062643905 created "2016-06-24" @default.
• W2062643905 creator A5013390724 @default.
• W2062643905 creator A5022370263 @default.
• W2062643905 creator A5069329638 @default.
• W2062643905 date "2001-03-22" @default.
• W2062643905 modified "2023-10-06" @default.
• W2062643905 title "Conformation of Myosin Interdomain Interactions during Contraction: Deductions from Proteins in Solution" @default.
• W2062643905 cites W1570071329 @default.
• W2062643905 cites W161645565 @default.
• W2062643905 cites W1877845887 @default.
• W2062643905 cites W1997236222 @default.
• W2062643905 cites W2003201132 @default.
• W2062643905 cites W2029734107 @default.
• W2062643905 cites W2044225421 @default.
• W2062643905 cites W2044629144 @default.
• W2062643905 cites W2049752561 @default.
• W2062643905 cites W2068074765 @default.
• W2062643905 cites W2077074448 @default.
• W2062643905 cites W2082276438 @default.
• W2062643905 cites W2125756645 @default.
• W2062643905 cites W2153435210 @default.
• W2062643905 cites W2154535641 @default.
• W2062643905 doi "https://doi.org/10.1021/bi002388g" @default.
• W2062643905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11294651" @default.
• W2062643905 hasPublicationYear "2001" @default.
• W2062643905 type Work @default.
• W2062643905 sameAs 2062643905 @default.
• W2062643905 citedByCount "8" @default.
• W2062643905 crossrefType "journal-article" @default.
• W2062643905 hasAuthorship W2062643905A5013390724 @default.
• W2062643905 hasAuthorship W2062643905A5022370263 @default.
• W2062643905 hasAuthorship W2062643905A5069329638 @default.
• W2062643905 hasConcept C105702510 @default.
• W2062643905 hasConcept C107524782 @default.
• W2062643905 hasConcept C120665830 @default.
• W2062643905 hasConcept C121332964 @default.
• W2062643905 hasConcept C121745418 @default.
• W2062643905 hasConcept C12554922 @default.
• W2062643905 hasConcept C125705527 @default.
• W2062643905 hasConcept C133571119 @default.
• W2062643905 hasConcept C15954220 @default.
• W2062643905 hasConcept C160408235 @default.
• W2062643905 hasConcept C181199279 @default.
• W2062643905 hasConcept C185592680 @default.
• W2062643905 hasConcept C23265538 @default.
• W2062643905 hasConcept C2776050358 @default.
• W2062643905 hasConcept C2777339483 @default.
• W2062643905 hasConcept C55493867 @default.
• W2062643905 hasConcept C62520636 @default.
• W2062643905 hasConcept C6997183 @default.
• W2062643905 hasConcept C71240020 @default.
• W2062643905 hasConcept C8010536 @default.
• W2062643905 hasConcept C8035138 @default.
• W2062643905 hasConcept C86803240 @default.
• W2062643905 hasConcept C91881484 @default.
• W2062643905 hasConceptScore W2062643905C105702510 @default.
• W2062643905 hasConceptScore W2062643905C107524782 @default.
• W2062643905 hasConceptScore W2062643905C120665830 @default.
• W2062643905 hasConceptScore W2062643905C121332964 @default.
• W2062643905 hasConceptScore W2062643905C121745418 @default.
• W2062643905 hasConceptScore W2062643905C12554922 @default.
• W2062643905 hasConceptScore W2062643905C125705527 @default.
• W2062643905 hasConceptScore W2062643905C133571119 @default.
• W2062643905 hasConceptScore W2062643905C15954220 @default.
• W2062643905 hasConceptScore W2062643905C160408235 @default.
• W2062643905 hasConceptScore W2062643905C181199279 @default.
• W2062643905 hasConceptScore W2062643905C185592680 @default.
• W2062643905 hasConceptScore W2062643905C23265538 @default.
• W2062643905 hasConceptScore W2062643905C2776050358 @default.
• W2062643905 hasConceptScore W2062643905C2777339483 @default.
• W2062643905 hasConceptScore W2062643905C55493867 @default.
• W2062643905 hasConceptScore W2062643905C62520636 @default.
• W2062643905 hasConceptScore W2062643905C6997183 @default.
• W2062643905 hasConceptScore W2062643905C71240020 @default.
• W2062643905 hasConceptScore W2062643905C8010536 @default.
• W2062643905 hasConceptScore W2062643905C8035138 @default.
• W2062643905 hasConceptScore W2062643905C86803240 @default.
• W2062643905 hasConceptScore W2062643905C91881484 @default.
• W2062643905 hasIssue "15" @default.
• W2062643905 hasLocation W20626439051 @default.
• W2062643905 hasLocation W20626439052 @default.
• W2062643905 hasOpenAccess W2062643905 @default.
• W2062643905 hasPrimaryLocation W20626439051 @default.
• W2062643905 hasRelatedWork W1679408412 @default.
• W2062643905 hasRelatedWork W1967427695 @default.
• W2062643905 hasRelatedWork W1974118613 @default.
• W2062643905 hasRelatedWork W2012444537 @default.
• W2062643905 hasRelatedWork W2030588184 @default.
• W2062643905 hasRelatedWork W2054684901 @default.
• W2062643905 hasRelatedWork W2072717059 @default.
• W2062643905 hasRelatedWork W2095633207 @default.
• W2062643905 hasRelatedWork W2126468848 @default.
• W2062643905 hasRelatedWork W2971244553 @default.
• W2062643905 hasVolume "40" @default.
• W2062643905 isParatext "false" @default.
• W2062643905 isRetracted "false" @default.
• W2062643905 magId "2062643905" @default.

• next