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- W2062678549 abstract "The application of high-performance liquid chromatography to the study of biogenic amine-related enzymes is reviewed. Biogenic amines include catecholamines (dopamine, norepinephrine and epinephrine), indoleamines (serotonin and melatonin), imidazoleamines (histamine), polyamines (putrescine, spermidine and spermine) and acetylcholine. Three particular aspects are covered. The first aspect is the assay of enzyme activities of biogenic amine-related enzymes, such as tyrosine hydroxylase, tryptophan hydroxylase, aromatic l-amino acid decarboxylase, dopamine β-hydroxylase and phenylethanolamine N-methyltransferase. The introduction of highly sensitive assays of biogenic amines with electrochemical detection or fluorescence detection have made possible the non-isotopic assay of these activities, replacing the previously used radioisotopic methods. The second aspect is the purification of these enzymes. Since biogenic amine-synthesizing enzymes are generally unstable, rapid and efficient purification of these enzymes is very useful. The third aspect is the assay of biogenic amines (for example, acetylcholine and polyamines) using post-column derivatization with biogenic amine oxidases and electrochemical detection." @default.
- W2062678549 created "2016-06-24" @default.
- W2062678549 creator A5004939550 @default.
- W2062678549 date "1991-05-01" @default.
- W2062678549 modified "2023-10-17" @default.
- W2062678549 title "Application of high-performance liquid chromatography to the study of biogenic amine-related enzymes" @default.
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- W2062678549 doi "https://doi.org/10.1016/0378-4347(91)80246-9" @default.
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