Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062687377> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2062687377 endingPage "126" @default.
- W2062687377 startingPage "121" @default.
- W2062687377 abstract "A novel plasmid expression system has been constructed that combines two useful functions: it facilitates single-step affinity purification of cytoplasmically overproduced fusion proteins and the in vitro processing of fusions with IgA protease (Igase). The significant features directing the high expression rate of pEV41-based gene fusions in Escherichia coli are the lambda pL promoter for temperature-regulated transcription and the translation initiation region of the bacteriophage MS2 polymerase gene including a downstream box (db) within the first few codons of the open reading frame. Fusion proteins generated with this system contain a short N-terminal carrier peptide allowing convenient affinity purification by means of the His6 peptide. As exemplified by the production of the variable heavy (VH) and light (VL)-chain domains of a monoclonal antibody, the fusion proteins can be specifically processed with Igase either in purified form or simply by incubation with the culture medium of recombinant E. coli [pJP10] cells. Chemical cross-linking of processed VH and VL domains resulted in a recombinant antibody Fv fragment that can specifically bind to its antigen." @default.
- W2062687377 created "2016-06-24" @default.
- W2062687377 creator A5037873316 @default.
- W2062687377 creator A5056159870 @default.
- W2062687377 creator A5079499882 @default.
- W2062687377 date "1993-08-01" @default.
- W2062687377 modified "2023-09-27" @default.
- W2062687377 title "A plasmid system for high-level expression and in vitro processing of recombinant proteins" @default.
- W2062687377 cites W1515935760 @default.
- W2062687377 cites W1806791563 @default.
- W2062687377 cites W1836449346 @default.
- W2062687377 cites W1973738093 @default.
- W2062687377 cites W1980640496 @default.
- W2062687377 cites W1982723373 @default.
- W2062687377 cites W1991693950 @default.
- W2062687377 cites W1994926732 @default.
- W2062687377 cites W2001705211 @default.
- W2062687377 cites W2003090737 @default.
- W2062687377 cites W2005579644 @default.
- W2062687377 cites W2007369397 @default.
- W2062687377 cites W2007930265 @default.
- W2062687377 cites W2008497514 @default.
- W2062687377 cites W2032118018 @default.
- W2062687377 cites W2035183715 @default.
- W2062687377 cites W2045117614 @default.
- W2062687377 cites W2054887464 @default.
- W2062687377 cites W2065465012 @default.
- W2062687377 cites W2067465835 @default.
- W2062687377 cites W2068446924 @default.
- W2062687377 cites W2070165671 @default.
- W2062687377 cites W2073517578 @default.
- W2062687377 cites W36310178 @default.
- W2062687377 doi "https://doi.org/10.1016/0378-1119(93)90354-6" @default.
- W2062687377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8344518" @default.
- W2062687377 hasPublicationYear "1993" @default.
- W2062687377 type Work @default.
- W2062687377 sameAs 2062687377 @default.
- W2062687377 citedByCount "34" @default.
- W2062687377 countsByYear W20626873772015 @default.
- W2062687377 crossrefType "journal-article" @default.
- W2062687377 hasAuthorship W2062687377A5037873316 @default.
- W2062687377 hasAuthorship W2062687377A5056159870 @default.
- W2062687377 hasAuthorship W2062687377A5079499882 @default.
- W2062687377 hasConcept C104317684 @default.
- W2062687377 hasConcept C111829193 @default.
- W2062687377 hasConcept C123894998 @default.
- W2062687377 hasConcept C153911025 @default.
- W2062687377 hasConcept C167625842 @default.
- W2062687377 hasConcept C22744801 @default.
- W2062687377 hasConcept C40767141 @default.
- W2062687377 hasConcept C47289529 @default.
- W2062687377 hasConcept C547475151 @default.
- W2062687377 hasConcept C55493867 @default.
- W2062687377 hasConcept C86803240 @default.
- W2062687377 hasConceptScore W2062687377C104317684 @default.
- W2062687377 hasConceptScore W2062687377C111829193 @default.
- W2062687377 hasConceptScore W2062687377C123894998 @default.
- W2062687377 hasConceptScore W2062687377C153911025 @default.
- W2062687377 hasConceptScore W2062687377C167625842 @default.
- W2062687377 hasConceptScore W2062687377C22744801 @default.
- W2062687377 hasConceptScore W2062687377C40767141 @default.
- W2062687377 hasConceptScore W2062687377C47289529 @default.
- W2062687377 hasConceptScore W2062687377C547475151 @default.
- W2062687377 hasConceptScore W2062687377C55493867 @default.
- W2062687377 hasConceptScore W2062687377C86803240 @default.
- W2062687377 hasIssue "1" @default.
- W2062687377 hasLocation W20626873771 @default.
- W2062687377 hasLocation W20626873772 @default.
- W2062687377 hasOpenAccess W2062687377 @default.
- W2062687377 hasPrimaryLocation W20626873771 @default.
- W2062687377 hasRelatedWork W1992556027 @default.
- W2062687377 hasRelatedWork W2008007771 @default.
- W2062687377 hasRelatedWork W2355848544 @default.
- W2062687377 hasRelatedWork W2359862199 @default.
- W2062687377 hasRelatedWork W2365121371 @default.
- W2062687377 hasRelatedWork W2371720847 @default.
- W2062687377 hasRelatedWork W2378877857 @default.
- W2062687377 hasRelatedWork W2384592831 @default.
- W2062687377 hasRelatedWork W2389269354 @default.
- W2062687377 hasRelatedWork W2973460871 @default.
- W2062687377 hasVolume "130" @default.
- W2062687377 isParatext "false" @default.
- W2062687377 isRetracted "false" @default.
- W2062687377 magId "2062687377" @default.
- W2062687377 workType "article" @default.