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• W2062720586 abstract "Paddy soil microbial community composition and functional diversity changes after a long-term fertilization were studied by BIOLOG. The results showed that the AWCD value eventually reached after a long-term fertilization was increased at some extent compared with the no fertilization. The diversity index results showed that the soil microbial community diversity was at a high level. The analysis for the utilization of carbon resources showed that communities were different on the utilization of carbon resource. Fungi and bacteria have obvious differences on the utilization of carbon resource and the activity ratio between fungi and bacteria was also different. Introduction The existing research evidences showed that fungi and bacteria’s influence to the decomposition of organic matter and nutrient release was different greatly. However, they occupy different circulation way in the ecosystem, influenced by different factors[1,2]. The assimilation efficiency on the organic carbon of Bacteria and its carbon reserves were both lower than fungi,so the relative composition and functional changes of fungi and bacteria in the microbial community are associated with organic carbon reserves. However, there is few research about function of fungal diversity[3]. For the sustainable agriculture,the way of reasonable fertilizer management is the kernel[4]. The existing research have shown that the use of organic fertilizer had a significant effect on soil microbial community and composition [5]. Berthrong found that the diversity of bacteria and fungi community are increasing by using organic fertilizer for 20 years[6]. This research will use the Biolog to study the effect of different fertilization treatment on the functional diversity of fungal community. Materials and methods The sample for the research. The long-term trial was set in institute of red soil in JinXian county Jiangxi province,and materials were the quaternary red clay type with a breeding paddy soil in red soil development, whose profile pattern was APWlW2G. The type of soil parent material was quaternary red clay, and the soil physical and chemical properties before the trial were shown in table 1. International Conference on Materials, Environmental and Biological Engineering (MEBE 2015) © 2015. The authors Published by Atlantis Press 36 Table 1. the soil physical and chemical properties genetic horizon Depth [cm] pH Organic [C/ g·kg] Whole [N/g·kg] Whole [P/g·kg] Hydrolys [N/mg.kg] quickactig [P/mg.kg] quickacting [K/mg.kg] Cosmid [/%] A 0~15 6.9 16.22 0.952 1.021 143.7 10.3 125.1 55.8 P 15~24 6.6 12.60 1.126 0.890 105.1 5.4 97.8 56.5 W1 24~38 6.2 3.751 0.692 0.618 37.0 1.6 54.0 58.4 W2 38~90 6.2 4.096 0.522 0.654 46.4 2.3 85.6 60.2 Experiment design. A total of six treatment were established for the experiment and the design was shown in table 2. The plot area was 60 m and arranged randomly with 3 repetitions. Table2. The experimental design for organic fertilizer positioning in the red paddy soil Treatment astragalus smicus pig manure straw MV 22 500 VM 22 500 22 500 VMS 22 500 22 500 4 500 VS 22 500 4 500 CF 160N, 16.4P, 100K CK CK(no fertilizer) Note:Chemical fertilizer was applied to treatment CF with urea (160 kg N·hm),calcium-magnesium phosphate (16.4 kg P·hm) and potassium chloride (100 kg K·hm) at early and late rice consistently during the experiment. The collection and treatment of samples. The undisturbed soil samples of paddy soil arable layer were taken (017cm)back to the laboratory and dried naturally in the middle of July, 2008. When the water content of soil reach to the plastic limit, you should cut gently large pieces of soil with your hand along the natural fractured surface,to exclude large plant residues and stones. And when it reaches 8 mm ,they should be sieved into the sealing bag after air dry constantly. Assay method. 5g soil sample (has been conversed into dry soil weight) was added to 45 mL 0.85% saline, and take them out after oscillation for 30 min. And they were stood for 10 min,then absorbed the soil solution, and after diluted 10 times, they were inoculated to the microwell plate, and the quantity of each hole is 125 μL. After inoculation for 17 d , the OD value was measured under 590nm wavelength. The tetrazolium blue indicator and carbon source should also be added into the microwell plate. In addition, the biological inhibitor streptomycin sulphate (0.1 g/mL), chlortetracycline (0.05 g/mL)were also needed to be added into it. The carbon sources included Dglucose, fructose,Dxylose, Larabinose, cellobiose, cellulose, Larginine, glycine, Lhistidine,Lserine, L-α-arginine, Lasp phthalein ammonia, Lsodium glutamate, sodium salicylate, gallic acid, tannic acid, vanillin, gelatin, gelatin, lignin and humic acid. Data analysis. Microlog 4.2 software platform was used to transform the data into a Microsoft Excel format, and they were analyzed in the Excel and SPSS17.0 statistical softwares. Results and analysis. There was significant difference for the utilization of carbon source under different fertilizer management. As can be seen from the figure 1, fungus's ability to use different" @default.
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• W2062720586 date "2015-01-01" @default.
• W2062720586 modified "2023-09-23" @default.
• W2062720586 title "The Effects of Paddy Soil Fungi Community Functional Diversity Under Organic Fertilizer Management Measures" @default.
• W2062720586 doi "https://doi.org/10.2991/mebe-15.2015.9" @default.
• W2062720586 hasPublicationYear "2015" @default.
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