Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062742793> ?p ?o ?g. }
- W2062742793 abstract "Previous studies of mixed background mice have demonstrated that targeted deletion of Vgf produces a lean, hypermetabolic mouse that is resistant to diet-, lesion-, and genetically-induced obesity. To investigate potential mechanism(s) and site(s) of action of VGF, a neuronal and endocrine secreted protein and neuropeptide precursor, we further analyzed the metabolic phenotypes of two independent VGF knockout lines on C57Bl6 backgrounds.Unlike hyperactive VGF knockout mice on a mixed C57Bl6-129/SvJ background, homozygous mutant mice on a C57Bl6 background were hypermetabolic with similar locomotor activity levels to Vgf+/Vgf+ mice, during day and night cycles, indicating that mechanism(s) other than hyperactivity were responsible for their increased energy expenditure. In Vgf-/Vgf- knockout mice, morphological analysis of brown and white adipose tissues (BAT and WAT) indicated decreased fat storage in both tissues, and decreased adipocyte perimeter and area in WAT. Changes in gene expression measured by real-time RT-PCR were consistent with increased fatty acid oxidation and uptake in BAT, and increased lipolysis, decreased lipogenesis, and brown adipocyte differentiation in WAT, suggesting that increased sympathetic nervous system activity in Vgf-/Vgf- mice may be associated with or responsible for alterations in energy expenditure and fat storage. In addition, uncoupling protein 1 (UCP1) and UCP2 protein levels, mitochondrial number, and mitochondrial cristae density were upregulated in Vgf-/Vgf- BAT. Using immunohistochemical and histochemical techniques, we detected VGF in nerve fibers innervating BAT and Vgf promoter-driven reporter expression in cervical and thoracic spinal ganglia that project to and innervate the chest wall and tissues including BAT. Moreover, VGF peptide levels were quantified by radioimmunoassay in BAT, and were found to be down-regulated by a high fat diet. Lastly, despite being hypermetabolic, VGF knockout mice were cold intolerant.We propose that VGF and/or VGF-derived peptides modulate sympathetic outflow pathways to regulate fat storage and energy expenditure." @default.
- W2062742793 created "2016-06-24" @default.
- W2062742793 creator A5003666844 @default.
- W2062742793 creator A5010821578 @default.
- W2062742793 creator A5020280521 @default.
- W2062742793 creator A5026140827 @default.
- W2062742793 creator A5047148881 @default.
- W2062742793 creator A5056368265 @default.
- W2062742793 creator A5070186157 @default.
- W2062742793 creator A5081308350 @default.
- W2062742793 date "2009-10-28" @default.
- W2062742793 modified "2023-10-04" @default.
- W2062742793 title "Analysis of knockout mice suggests a role for VGF in the control of fat storage and energy expenditure" @default.
- W2062742793 cites W128027390 @default.
- W2062742793 cites W1495750604 @default.
- W2062742793 cites W1594976448 @default.
- W2062742793 cites W1597982565 @default.
- W2062742793 cites W1631449371 @default.
- W2062742793 cites W1672804368 @default.
- W2062742793 cites W1734831379 @default.
- W2062742793 cites W184177557 @default.
- W2062742793 cites W1917754098 @default.
- W2062742793 cites W1945571160 @default.
- W2062742793 cites W1946955521 @default.
- W2062742793 cites W1952746940 @default.
- W2062742793 cites W1966695179 @default.
- W2062742793 cites W1967359474 @default.
- W2062742793 cites W1967896645 @default.
- W2062742793 cites W1970774395 @default.
- W2062742793 cites W1971116733 @default.
- W2062742793 cites W1975472203 @default.
- W2062742793 cites W1975515886 @default.
- W2062742793 cites W1976555898 @default.
- W2062742793 cites W1982232718 @default.
- W2062742793 cites W1989642979 @default.
- W2062742793 cites W1990056505 @default.
- W2062742793 cites W1992485652 @default.
- W2062742793 cites W1996039273 @default.
- W2062742793 cites W1998034140 @default.
- W2062742793 cites W1999230112 @default.
- W2062742793 cites W1999990598 @default.
- W2062742793 cites W2000147383 @default.
- W2062742793 cites W2002709344 @default.
- W2062742793 cites W2004144873 @default.
- W2062742793 cites W2012275690 @default.
- W2062742793 cites W2013460786 @default.
- W2062742793 cites W2013583119 @default.
- W2062742793 cites W2014093920 @default.
- W2062742793 cites W2014557719 @default.
- W2062742793 cites W2014697141 @default.
- W2062742793 cites W2025530025 @default.
- W2062742793 cites W2026010779 @default.
- W2062742793 cites W2027880941 @default.
- W2062742793 cites W2028558940 @default.
- W2062742793 cites W2028800124 @default.
- W2062742793 cites W2031724207 @default.
- W2062742793 cites W2032543649 @default.
- W2062742793 cites W2033725363 @default.
- W2062742793 cites W2038126428 @default.
- W2062742793 cites W2039337067 @default.
- W2062742793 cites W2040163240 @default.
- W2062742793 cites W2044585537 @default.
- W2062742793 cites W2045205233 @default.
- W2062742793 cites W2047687395 @default.
- W2062742793 cites W2054806352 @default.
- W2062742793 cites W2054900249 @default.
- W2062742793 cites W2056318154 @default.
- W2062742793 cites W2058799896 @default.
- W2062742793 cites W2058936782 @default.
- W2062742793 cites W2059476202 @default.
- W2062742793 cites W2060682066 @default.
- W2062742793 cites W2065987916 @default.
- W2062742793 cites W2068863408 @default.
- W2062742793 cites W2076310797 @default.
- W2062742793 cites W2077772069 @default.
- W2062742793 cites W2078194360 @default.
- W2062742793 cites W2078989286 @default.
- W2062742793 cites W2079554070 @default.
- W2062742793 cites W2085792819 @default.
- W2062742793 cites W2095951402 @default.
- W2062742793 cites W2096551871 @default.
- W2062742793 cites W2103936628 @default.
- W2062742793 cites W2104751353 @default.
- W2062742793 cites W2109861165 @default.
- W2062742793 cites W2110644487 @default.
- W2062742793 cites W2111784334 @default.
- W2062742793 cites W2115204044 @default.
- W2062742793 cites W2120327938 @default.
- W2062742793 cites W2125851390 @default.
- W2062742793 cites W2125914461 @default.
- W2062742793 cites W2131431703 @default.
- W2062742793 cites W2131716859 @default.
- W2062742793 cites W2133261364 @default.
- W2062742793 cites W2136803357 @default.
- W2062742793 cites W2139223838 @default.
- W2062742793 cites W2141060229 @default.
- W2062742793 cites W2141401983 @default.
- W2062742793 cites W2146586426 @default.
- W2062742793 cites W2151566924 @default.
- W2062742793 cites W2152339157 @default.