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- W2062783447 abstract "Abstract Ambisexual teleost fish are the only vertebrates among which either simultaneous hermaphroditism or spontaneous sex inversion normally occur. In some cases both heterologous germinal tissues coexist during a certain period of the animal's life cycle and can be separated mechanically from each other. Under these conditions metabolic changes during the reproductive cycle and eventual alterations related to sex inversion can be investigated. From samples of this kind sliced tissues have been incubated with C14-labeled steroid precursors (progesterone, testosterone) for 2 h at 26°C without adding cofactors. Testicular tissue from the simultaneous hermaphrodite Serranus cabrilla, the protandric Pagellus acarne and the diandric protogynous Coris julis have been used in the experiments. The metabolites were separated by paper- and thin layer-chromatography and identified by derivative formation and crystallization to constant specific activity. Besides the formation of various saturated 5β-reduced C19- and C21-steroids the occurrence of Δ4-steroids with a hydroxyl- or oxo-group at C-11 including 11-hydroxy-progesterone is remarkable. Differences in metabolic patterns are more important comparing different species than tissues of the same animal at various physiological stages." @default.
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- W2062783447 date "1975-03-01" @default.
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- W2062783447 title "In vitro studies on steroid metabolism of testicular tissue in ambisexual teleost fish" @default.
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- W2062783447 doi "https://doi.org/10.1016/0022-4731(75)90153-3" @default.
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