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- W2062823786 abstract "Gene expression during gastrulation in porcine embryos has been sparsely studied, but there are indications that species-specific patterns exist. Here, we investigated the three-dimensional (3D) expression of the T-box transcription factor Brachyury (T) and the forkhead box transcription factor FOXA2 by immunocytochemistry in porcine peri-gastrulation embryos. The first T(+) cells were detected in posterior epiblast of ovoid blastocysts. Later T(+) FOXA2(-) cells were found in the posterior primitive streak (PS) and nascent mesoderm, T(+) FOXA2(+) cells in the anterior PS, probably identifying the organizer region, and T(-) FOXA2(+) cells anterior to this region. In embryos with a neural groove, T and FOXA2 were co-expressed in the node and notochord, FOXA2 was expressed in the floor plate and posteriorly T was expressed in the streak. In all developmental stages, FOXA2 was expressed in the entire hypoblast/definitive endoderm. We conclude that the expression pattern of T and FOXA2 is largely conserved between pig and mouse." @default.
- W2062823786 created "2016-06-24" @default.
- W2062823786 creator A5006662295 @default.
- W2062823786 creator A5008188163 @default.
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- W2062823786 date "2011-03-10" @default.
- W2062823786 modified "2023-10-05" @default.
- W2062823786 title "Three-dimensional immunohistochemical characterization of lineage commitment by localization of T and FOXA2 in porcine peri-implantation embryos" @default.
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- W2062823786 doi "https://doi.org/10.1002/dvdy.22602" @default.
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