FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062828595> ?p ?o ?g. }

• W2062828595 endingPage "32" @default.
• W2062828595 startingPage "20" @default.
• W2062828595 abstract "Autophagic responses to chemotherapeutic agents may vary greatly among different prostate cancer cells and have not been well characterized. In this study, we showed that valproic acid (VPA) induced conversion of LC3-I to LC3-II and formation of LC3 puncta, the typical markers of autophagy, in LNCaP and PC-3 cells. However, these markers were undetectable in DU145 cells upon autophagic stimulation, indicating a defect of autophagy in this cell line. Among several critical autophagy-related proteins, ATG5 and ATG12-ATG5 conjugates, which are essential for autophagy induction, were absent in DU145 cells. No canonical transcripts for full-length ATG5 but only two alternatively spliced ATG5 transcripts were identified in DU145 cells. These alternative transcripts lack one or two exons, leading to premature termination of ATG5 translation. Transfection of the wild-type ATG5 gene into DU145 cells rescued the production of ATG5 and ATG12-ATG5 conjugates, resulting in formation of LC3-II conjugates and LC3 puncta. Moreover, the levels of the SQSTM1 protein, which should be degradable as an autophagy adaptor, were much higher in DU145 than in LNCaP and PC-3 cells, but were significantly decreased after ATG5 restoration in DU145 cells. However, expression of wild-type ATG5 in DU145 or knockdown of ATG5 in LNCaP and PC-3 cells did not change the inhibitory effects of VPA on these cells. Collectively, these results indicated that VPA-induced autophagy in prostate cancer cells depended on ATG5 and more importantly, that the autophagy pathway was genetically impaired in DU145 cells, suggesting caution in interpreting autophagic responses in this cell line." @default.
• W2062828595 created "2016-06-24" @default.
• W2062828595 creator A5005894428 @default.
• W2062828595 creator A5028926069 @default.
• W2062828595 creator A5032268960 @default.
• W2062828595 creator A5052703331 @default.
• W2062828595 creator A5053958928 @default.
• W2062828595 creator A5082382923 @default.
• W2062828595 creator A5087653918 @default.
• W2062828595 date "2013-01-09" @default.
• W2062828595 modified "2023-10-16" @default.
• W2062828595 title "Autophagy is differentially induced in prostate cancer LNCaP, DU145 and PC-3 cells via distinct splicing profiles of ATG5" @default.
• W2062828595 cites W1973729541 @default.
• W2062828595 cites W1974099441 @default.
• W2062828595 cites W1978285826 @default.
• W2062828595 cites W1980626135 @default.
• W2062828595 cites W1981705266 @default.
• W2062828595 cites W1981816686 @default.
• W2062828595 cites W1983788571 @default.
• W2062828595 cites W1992532731 @default.
• W2062828595 cites W1997714593 @default.
• W2062828595 cites W2000309242 @default.
• W2062828595 cites W2012040176 @default.
• W2062828595 cites W2014918593 @default.
• W2062828595 cites W2015886726 @default.
• W2062828595 cites W2021400058 @default.
• W2062828595 cites W2023097049 @default.
• W2062828595 cites W2037320436 @default.
• W2062828595 cites W2037670899 @default.
• W2062828595 cites W2039139796 @default.
• W2062828595 cites W2040157483 @default.
• W2062828595 cites W2040283429 @default.
• W2062828595 cites W2044573387 @default.
• W2062828595 cites W2049528479 @default.
• W2062828595 cites W2064064392 @default.
• W2062828595 cites W2064310618 @default.
• W2062828595 cites W2075958941 @default.
• W2062828595 cites W2077397852 @default.
• W2062828595 cites W2080049682 @default.
• W2062828595 cites W2080266689 @default.
• W2062828595 cites W2080846915 @default.
• W2062828595 cites W2082804884 @default.
• W2062828595 cites W2085053844 @default.
• W2062828595 cites W2086853865 @default.
• W2062828595 cites W2089519022 @default.
• W2062828595 cites W2094698940 @default.
• W2062828595 cites W2103339779 @default.
• W2062828595 cites W2106255449 @default.
• W2062828595 cites W2107114877 @default.
• W2062828595 cites W2122031852 @default.
• W2062828595 cites W2125249450 @default.
• W2062828595 cites W2128272175 @default.
• W2062828595 cites W2128304537 @default.
• W2062828595 cites W2130255188 @default.
• W2062828595 cites W2131220810 @default.
• W2062828595 cites W2133789233 @default.
• W2062828595 cites W2141165347 @default.
• W2062828595 cites W2141976464 @default.
• W2062828595 cites W2149771125 @default.
• W2062828595 cites W2161234475 @default.
• W2062828595 cites W2167256217 @default.
• W2062828595 doi "https://doi.org/10.4161/auto.22397" @default.
• W2062828595 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3542215" @default.
• W2062828595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23075929" @default.
• W2062828595 hasPublicationYear "2013" @default.
• W2062828595 type Work @default.
• W2062828595 sameAs 2062828595 @default.
• W2062828595 citedByCount "97" @default.
• W2062828595 countsByYear W20628285952013 @default.
• W2062828595 countsByYear W20628285952014 @default.
• W2062828595 countsByYear W20628285952015 @default.
• W2062828595 countsByYear W20628285952016 @default.
• W2062828595 countsByYear W20628285952017 @default.
• W2062828595 countsByYear W20628285952018 @default.
• W2062828595 countsByYear W20628285952019 @default.
• W2062828595 countsByYear W20628285952020 @default.
• W2062828595 countsByYear W20628285952021 @default.
• W2062828595 countsByYear W20628285952022 @default.
• W2062828595 countsByYear W20628285952023 @default.
• W2062828595 crossrefType "journal-article" @default.
• W2062828595 hasAuthorship W2062828595A5005894428 @default.
• W2062828595 hasAuthorship W2062828595A5028926069 @default.
• W2062828595 hasAuthorship W2062828595A5032268960 @default.
• W2062828595 hasAuthorship W2062828595A5052703331 @default.
• W2062828595 hasAuthorship W2062828595A5053958928 @default.
• W2062828595 hasAuthorship W2062828595A5082382923 @default.
• W2062828595 hasAuthorship W2062828595A5087653918 @default.
• W2062828595 hasBestOaLocation W20628285951 @default.
• W2062828595 hasConcept C121608353 @default.
• W2062828595 hasConcept C144027150 @default.
• W2062828595 hasConcept C190283241 @default.
• W2062828595 hasConcept C203522944 @default.
• W2062828595 hasConcept C2776438761 @default.
• W2062828595 hasConcept C2779723316 @default.
• W2062828595 hasConcept C2780329694 @default.
• W2062828595 hasConcept C2781000418 @default.
• W2062828595 hasConcept C2781035296 @default.
• W2062828595 hasConcept C502942594 @default.

• next