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- W2062833874 abstract "In animal ribosomes, two stalk proteins P1 and P2 form a heterodimer, and the two dimers, with the anchor protein P0, constitute a pentameric complex crucial for recruitment of translational GTPase factors to the ribosome. To investigate the functional contribution of each copy of the stalk proteins, we constructed P0 mutants, in which one of the two C-terminal helices, namely helix I (N-terminal side) or helix II (C-terminal side) were unable to bind the P1-P2 dimer. We also constructed 'one-C-terminal domain (CTD) stalk dimers', P1-P2ΔC and P1ΔC-P2, composed of intact P1/P2 monomer and a CTD-truncated partner. Through combinations of P0 and P1-P2 variants, various complexes were reconstituted and their function tested in eEF-2-dependent GTPase and eEF-1α/eEF-2-dependent polyphenylalanine synthesis assays in vitro. Double/single-CTD dimers bound to helix I showed higher activity than that bound to helix II. Despite low polypeptide synthetic activity by a single one-CTD dimer, its binding to both helices considerably increased activity, suggesting that two stalk dimers cooperate, particularly in polypeptide synthesis. This promotion of activity by two stalk dimers was lost upon mutation of the conserved YPT sequence connecting the two helices of P0, suggesting a role for this sequence in cooperativity of two stalk dimers." @default.
- W2062833874 created "2016-06-24" @default.
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- W2062833874 date "2013-02-01" @default.
- W2062833874 modified "2023-10-18" @default.
- W2062833874 title "Molecular dissection of the silkworm ribosomal stalk complex: the role of multiple copies of the stalk proteins" @default.
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- W2062833874 doi "https://doi.org/10.1093/nar/gkt044" @default.
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