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- W2062835601 abstract "Promoter-cloning plasmids were constructed and have been used to isolate transcriptionally active DNA fragments from Staphylococcus aureus. The plasmids contain a chloramphenicol acetyltransferase (CAT) gene of Gram-positive (G+) origin which lacks both its promoter and the sequence responsible for CAT inducibility. The ability of S. aureus promoters to direct CAT expression in Escherichia coli and Bacillus subtilis was examined. Two classes of staphylococcal promoter sequences have been obtained. Class I DNA fragments direct CAT expression in S. aureus, B. subtilis, and E. coli, while class II DNA sequences direct CAT expression only in the G+ hosts." @default.
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- W2062835601 date "1986-01-01" @default.
- W2062835601 modified "2023-10-18" @default.
- W2062835601 title "Differential utilization of Staphylococcus aureus promoter sequences by Escherichia coli and Bacillus subtilis" @default.
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- W2062835601 doi "https://doi.org/10.1016/0378-1119(86)90355-0" @default.
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