FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062870024> ?p ?o ?g. }

• W2062870024 endingPage "433" @default.
• W2062870024 startingPage "422" @default.
• W2062870024 abstract "Prerequisites of developing in vitro studies for a better understanding of the control mechanisms underlying the proliferation and differentiation of spermatogonia (Go) in the teleost testis are: (1) to be able to identify the different types of Go; (2) to maintain in culture the structural relationships occurring in situ between the various testicular cell types, as intact as possible; and (3) to know how the Go survive and proliferate in culture for several days. After very gentle homogenization of trout testes treated with collagenase, a cell suspension containing mainly spermatocysts (one or several Sertoli cells enclosing one Go or a clone of germ cells) and clusters of myoid cells and Leydig cells was seeded in culture onto a laminin plus fibronectin coating. After 4.5–6 days in culture, then staining with May-Grünwald and Giemsa reagents, the determination of the nuclear and cellular size of the various Go and of the number of Go present in clones has enabled the identification of two types of large Go, in pairs or alone (Go A) and six successive types of smaller Go (Go B). Cell viability determination by staining with Rhodamine 123/propidium iodide (PI)/Hoechst 33342 and with FITC-Annexin V/PI indicated that after 5–7 days in culture, all the somatic cells and most of the Go were viable. Only some of the Go, mainly among the most differentiated ones, underwent apoptosis, as it was the case for a number of spermatocytes and spermatids increasing with the time in culture. Brdu labelling and 3H-Thymidine (3H-Tdr) incorporation indicated that the proliferative activity of Go was at a maximum after 4.5 days in culture and that the response to at least two molecules (QAYL-IGF-I and GTH-I) remained unchanged between 3 and 6 days. As only very scarce somatic cells from immature/spermatogenetic testes synthesized DNA up to 6 days in culture, the measurement of 3H-Tdr incorporation by cells from such testes reliably reflected synthesis of DNA by only the Go (and eventually also by primary spermatocytes when they are present). In conclusion, this study provides information allowing a detailed analysis of the events related with the mitotic phase of spermatogenesis in the trout and it establishes that primary cultures of testicular cells carried out in the reported conditions represent a useful tool to develop an analysis of the mechanisms participating in the control of this phase. Mol. Reprod. Dev. 53:422–433, 1999. © 1999 Wiley-Liss, Inc." @default.
• W2062870024 created "2016-06-24" @default.
• W2062870024 creator A5078915503 @default.
• W2062870024 date "1999-08-01" @default.
• W2062870024 modified "2023-10-04" @default.
• W2062870024 title "Spermatogonia of rainbow trout: I. morphological characterization, mitotic activity, and survival in primary cultures of testicular cells" @default.
• W2062870024 cites W1772725371 @default.
• W2062870024 cites W1986858409 @default.
• W2062870024 cites W1993203184 @default.
• W2062870024 cites W1994285818 @default.
• W2062870024 cites W2005437197 @default.
• W2062870024 cites W2006678706 @default.
• W2062870024 cites W2016998480 @default.
• W2062870024 cites W2027476296 @default.
• W2062870024 cites W2036937264 @default.
• W2062870024 cites W2039761939 @default.
• W2062870024 cites W2041668725 @default.
• W2062870024 cites W2049123043 @default.
• W2062870024 cites W2052254151 @default.
• W2062870024 cites W2058145364 @default.
• W2062870024 cites W2059153388 @default.
• W2062870024 cites W2061328769 @default.
• W2062870024 cites W2076549599 @default.
• W2062870024 cites W2078532650 @default.
• W2062870024 cites W2080524329 @default.
• W2062870024 cites W2083318002 @default.
• W2062870024 cites W2095058289 @default.
• W2062870024 cites W2098400794 @default.
• W2062870024 cites W2123387315 @default.
• W2062870024 cites W2124479860 @default.
• W2062870024 cites W2124559417 @default.
• W2062870024 cites W2124649490 @default.
• W2062870024 cites W2125988841 @default.
• W2062870024 cites W2137022580 @default.
• W2062870024 cites W2144325594 @default.
• W2062870024 cites W2158161476 @default.
• W2062870024 cites W2163970711 @default.
• W2062870024 doi "https://doi.org/10.1002/(sici)1098-2795(199908)53:4<422::aid-mrd8>3.0.co;2-v" @default.
• W2062870024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10398418" @default.
• W2062870024 hasPublicationYear "1999" @default.
• W2062870024 type Work @default.
• W2062870024 sameAs 2062870024 @default.
• W2062870024 citedByCount "41" @default.
• W2062870024 countsByYear W20628700242012 @default.
• W2062870024 countsByYear W20628700242013 @default.
• W2062870024 countsByYear W20628700242014 @default.
• W2062870024 countsByYear W20628700242016 @default.
• W2062870024 countsByYear W20628700242017 @default.
• W2062870024 countsByYear W20628700242018 @default.
• W2062870024 countsByYear W20628700242019 @default.
• W2062870024 countsByYear W20628700242020 @default.
• W2062870024 countsByYear W20628700242021 @default.
• W2062870024 countsByYear W20628700242023 @default.
• W2062870024 crossrefType "journal-article" @default.
• W2062870024 hasAuthorship W2062870024A5078915503 @default.
• W2062870024 hasConcept C104317684 @default.
• W2062870024 hasConcept C123765429 @default.
• W2062870024 hasConcept C134018914 @default.
• W2062870024 hasConcept C134305767 @default.
• W2062870024 hasConcept C1491633281 @default.
• W2062870024 hasConcept C153911025 @default.
• W2062870024 hasConcept C16685009 @default.
• W2062870024 hasConcept C181199279 @default.
• W2062870024 hasConcept C189014844 @default.
• W2062870024 hasConcept C190283241 @default.
• W2062870024 hasConcept C203014093 @default.
• W2062870024 hasConcept C2775934118 @default.
• W2062870024 hasConcept C2778394429 @default.
• W2062870024 hasConcept C2781068068 @default.
• W2062870024 hasConcept C31573885 @default.
• W2062870024 hasConcept C54355233 @default.
• W2062870024 hasConcept C55493867 @default.
• W2062870024 hasConcept C71924100 @default.
• W2062870024 hasConcept C74864618 @default.
• W2062870024 hasConcept C81885089 @default.
• W2062870024 hasConcept C86803240 @default.
• W2062870024 hasConcept C95444343 @default.
• W2062870024 hasConceptScore W2062870024C104317684 @default.
• W2062870024 hasConceptScore W2062870024C123765429 @default.
• W2062870024 hasConceptScore W2062870024C134018914 @default.
• W2062870024 hasConceptScore W2062870024C134305767 @default.
• W2062870024 hasConceptScore W2062870024C1491633281 @default.
• W2062870024 hasConceptScore W2062870024C153911025 @default.
• W2062870024 hasConceptScore W2062870024C16685009 @default.
• W2062870024 hasConceptScore W2062870024C181199279 @default.
• W2062870024 hasConceptScore W2062870024C189014844 @default.
• W2062870024 hasConceptScore W2062870024C190283241 @default.
• W2062870024 hasConceptScore W2062870024C203014093 @default.
• W2062870024 hasConceptScore W2062870024C2775934118 @default.
• W2062870024 hasConceptScore W2062870024C2778394429 @default.
• W2062870024 hasConceptScore W2062870024C2781068068 @default.
• W2062870024 hasConceptScore W2062870024C31573885 @default.
• W2062870024 hasConceptScore W2062870024C54355233 @default.
• W2062870024 hasConceptScore W2062870024C55493867 @default.
• W2062870024 hasConceptScore W2062870024C71924100 @default.
• W2062870024 hasConceptScore W2062870024C74864618 @default.
• W2062870024 hasConceptScore W2062870024C81885089 @default.
• W2062870024 hasConceptScore W2062870024C86803240 @default.

• next