Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062877356> ?p ?o ?g. }
- W2062877356 endingPage "e15876" @default.
- W2062877356 startingPage "e15876" @default.
- W2062877356 abstract "Autoinducer-2 (AI-2) mediated quorum sensing has been associated with the expression of virulence factors in a number of pathogenic organisms and has been demonstrated to play a role in motility and cytolethal distending toxin (cdt) production in Campylobacter jejuni. We have initiated the work to determine the molecular basis of AI-2 synthesis and the biological functions of quorum sensing in C. jejuni. In this work, two naturally occurring variants of C. jejuni 81116 were identified, one producing high-levels of AI-2 while the other is defective in AI-2 synthesis. Sequence analysis revealed a G92D mutation in the luxS gene of the defective variant. Complementation of the AI-2(-) variant with a plasmid encoded copy of the wild-type luxS gene or reversion of the G92D mutation by site-directed mutagenesis fully restored AI-2 production by the variant. These results indicate that the G92D mutation alone is responsible for the loss of AI-2 activity in C. jejuni. Kinetic analyses showed that the G92D LuxS has a ∼100-fold reduced catalytic activity relative to the wild-type enzyme. Findings from this study identify a previously undescribed amino acid that is essential for AI-2 production by LuxS and provide a unique isogenic pair of naturally occurring variants for us to dissect the functions of AI-2 mediated quorum sensing in Campylobacter." @default.
- W2062877356 created "2016-06-24" @default.
- W2062877356 creator A5027984887 @default.
- W2062877356 creator A5052833351 @default.
- W2062877356 creator A5061706391 @default.
- W2062877356 creator A5063942589 @default.
- W2062877356 creator A5086172106 @default.
- W2062877356 date "2011-01-11" @default.
- W2062877356 modified "2023-10-18" @default.
- W2062877356 title "Identification of a Key Amino Acid of LuxS Involved in AI-2 Production in Campylobacter jejuni" @default.
- W2062877356 cites W1548495754 @default.
- W2062877356 cites W1899978364 @default.
- W2062877356 cites W1982580939 @default.
- W2062877356 cites W1986172983 @default.
- W2062877356 cites W1996135784 @default.
- W2062877356 cites W1996872292 @default.
- W2062877356 cites W2005874691 @default.
- W2062877356 cites W2031713442 @default.
- W2062877356 cites W2035337053 @default.
- W2062877356 cites W2040126095 @default.
- W2062877356 cites W2043805760 @default.
- W2062877356 cites W2046899957 @default.
- W2062877356 cites W2048622484 @default.
- W2062877356 cites W2058776820 @default.
- W2062877356 cites W2060779329 @default.
- W2062877356 cites W2060816398 @default.
- W2062877356 cites W2061257637 @default.
- W2062877356 cites W2068893770 @default.
- W2062877356 cites W2072595740 @default.
- W2062877356 cites W2073288136 @default.
- W2062877356 cites W2082097885 @default.
- W2062877356 cites W2083628471 @default.
- W2062877356 cites W2084947014 @default.
- W2062877356 cites W2087193563 @default.
- W2062877356 cites W2095655760 @default.
- W2062877356 cites W2095783952 @default.
- W2062877356 cites W2103359264 @default.
- W2062877356 cites W2104571730 @default.
- W2062877356 cites W2104941420 @default.
- W2062877356 cites W2107508284 @default.
- W2062877356 cites W2111352717 @default.
- W2062877356 cites W2112411547 @default.
- W2062877356 cites W2120904752 @default.
- W2062877356 cites W2122918147 @default.
- W2062877356 cites W2127812294 @default.
- W2062877356 cites W2142250977 @default.
- W2062877356 cites W2144111851 @default.
- W2062877356 cites W2144640625 @default.
- W2062877356 cites W2162863229 @default.
- W2062877356 cites W2164892862 @default.
- W2062877356 cites W2167637231 @default.
- W2062877356 cites W2169400224 @default.
- W2062877356 cites W2170798409 @default.
- W2062877356 cites W2170964260 @default.
- W2062877356 cites W2171410721 @default.
- W2062877356 doi "https://doi.org/10.1371/journal.pone.0015876" @default.
- W2062877356 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3019222" @default.
- W2062877356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21264316" @default.
- W2062877356 hasPublicationYear "2011" @default.
- W2062877356 type Work @default.
- W2062877356 sameAs 2062877356 @default.
- W2062877356 citedByCount "29" @default.
- W2062877356 countsByYear W20628773562012 @default.
- W2062877356 countsByYear W20628773562013 @default.
- W2062877356 countsByYear W20628773562014 @default.
- W2062877356 countsByYear W20628773562015 @default.
- W2062877356 countsByYear W20628773562018 @default.
- W2062877356 countsByYear W20628773562020 @default.
- W2062877356 countsByYear W20628773562021 @default.
- W2062877356 countsByYear W20628773562022 @default.
- W2062877356 countsByYear W20628773562023 @default.
- W2062877356 crossrefType "journal-article" @default.
- W2062877356 hasAuthorship W2062877356A5027984887 @default.
- W2062877356 hasAuthorship W2062877356A5052833351 @default.
- W2062877356 hasAuthorship W2062877356A5061706391 @default.
- W2062877356 hasAuthorship W2062877356A5063942589 @default.
- W2062877356 hasAuthorship W2062877356A5086172106 @default.
- W2062877356 hasBestOaLocation W20628773561 @default.
- W2062877356 hasConcept C104317684 @default.
- W2062877356 hasConcept C118687296 @default.
- W2062877356 hasConcept C143065580 @default.
- W2062877356 hasConcept C16318435 @default.
- W2062877356 hasConcept C188082640 @default.
- W2062877356 hasConcept C22744801 @default.
- W2062877356 hasConcept C2776957140 @default.
- W2062877356 hasConcept C2779910956 @default.
- W2062877356 hasConcept C501734568 @default.
- W2062877356 hasConcept C523546767 @default.
- W2062877356 hasConcept C54355233 @default.
- W2062877356 hasConcept C60987743 @default.
- W2062877356 hasConcept C86803240 @default.
- W2062877356 hasConcept C89423630 @default.
- W2062877356 hasConceptScore W2062877356C104317684 @default.
- W2062877356 hasConceptScore W2062877356C118687296 @default.
- W2062877356 hasConceptScore W2062877356C143065580 @default.
- W2062877356 hasConceptScore W2062877356C16318435 @default.
- W2062877356 hasConceptScore W2062877356C188082640 @default.
- W2062877356 hasConceptScore W2062877356C22744801 @default.