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- W2062902956 abstract "Polarization methods, introduced in the 1800s, offered one of the earliest ways to examine protein structure. Since then, many other structure-sensitive probes have been developed, but circular dichroism (CD) remains a powerful technique because of its versatility and the specificity of protein structural information that can be explored. With improvements in time resolution, from millisecond to picosecond CD measurements, it has proven to be an important tool for studying the mechanism of folding and function in many biomolecules. For example, nanosecond time-resolved CD (TRCD) studies of the sub-microsecond events of reduced cytochrome c folding have provided direct experimental evidence of kinetic heterogeneity, which is an inherent property of the diffusional nature of early folding dynamics on the energy landscape. In addition, TRCD has been applied to the study of many biochemical processes, such as ligand rebinding in hemoglobin and myoglobin and signaling state formation in photoactive yellow protein and prototropin 1 LOV2. The basic approach to TRCD has also been extended to include a repertoire of nanosecond polarization spectroscopies: optical rotatory dispersion (ORD), magnetic CD and ORD, and linear dichroism. This article will discuss the details of the polarization methods used in this laboratory, as well as the coupling of time-resolved ORD with the temperature-jump trigger so that protein folding can be studied in a larger number of proteins." @default.
- W2062902956 created "2016-06-24" @default.
- W2062902956 creator A5038140081 @default.
- W2062902956 creator A5045222853 @default.
- W2062902956 creator A5067894240 @default.
- W2062902956 date "2010-09-01" @default.
- W2062902956 modified "2023-10-15" @default.
- W2062902956 title "Nanosecond time-resolved polarization spectroscopies: Tools for probing protein reaction mechanisms" @default.
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- W2062902956 doi "https://doi.org/10.1016/j.ymeth.2010.04.015" @default.
- W2062902956 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2934884" @default.
- W2062902956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20438842" @default.
- W2062902956 hasPublicationYear "2010" @default.