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• W2062912445 abstract "Abstract Experiments are described showing the feasibility of electrical communication between the enzyme glucose oxidase and a ferrocene redox mediator when both are incorporated into a (Nafion®) perfluorosulfonic acid cation-exchange polymer film coated on an electrode. It was shown that Nafion® films can serve as a stable immobilization matrix that is compatible with the conditions required for an enzyme catalyst to function. An experimental strategy for the incorporation of a redox mediator and the enzyme into the ion-exchange polymer film is described. This involved casting mixed solutions of enzyme + mediator and Nafion® onto an electrode surface. The ferrocene mediator was chemically modified so that it was strongly retained inside Nafion® through hydrophobic interactions. In the presence of glucose, the mediator was able to transfer electrons from the redox center of the film-entrapped glucose oxidase to the electrode. Slow rates of charge transfer between the enzyme and the electrode, because of the high affinity of the modified mediator for the polymer, is a difficulty with this method at present. An extension of the concepts demonstrated in this paper could lead to practical enzyme electrodes for analytical purposes." @default.
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• W2062912445 date "1993-10-01" @default.
• W2062912445 modified "2023-09-27" @default.
• W2062912445 title "Enzyme electrodes based on ionomer films coated on electrodes" @default.
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• W2062912445 doi "https://doi.org/10.1016/0022-0728(93)80384-t" @default.
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