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• W2062912792 abstract "1. In isolated pig detrusor and vesical arterial smooth muscle preparations, endothelin-1 (ET-1) caused concentration- dependent contractions. Nifedipine (10−6 M) did not significantly affect the action of ET-1 in the vessels, but almost abolished its effect in the detrusor. Incubation for 30 min in Ca2+-free solution markedly reduced the ET-1-induced contractions in both detrusor and vesical arteries. 2. The protein kinase C inhibitor H-7 (3 × 10−5 M), reduced the response to ET-1 in detrusor muscle as well as in vessels, and abolished the contractions evoked by ET-1 in Ca2+-free solution. 3. ET-1 caused an increase in the accumulation of inositol phosphates (IPs) in preparations prelabelled with myo-[3H]inositol. After exposure to ET-1 (10−7 M) for 60 min, an approx. 4-fold increase in IPs levels were demonstrated, compared to untreated controls, in both detrusor and vessel preparations. Pretreatment with nifedipine (10−6 M) did not reduce IPs formation. In contrast, no increase in IPs formation was demonstrated in Ca2+-free medium. 4. The increase in accumulation of IPs was slow in onset in both detrusor and vesical arteries, with no significant accumulation demonstrable during the first 30 min. Time-course studies of tension development for ET-1 revealed that maximum tension was reached before significant levels of IPs could be detected." @default.
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• W2062912792 date "1992-05-01" @default.
• W2062912792 modified "2023-09-26" @default.
• W2062912792 title "Endothelin-1-induced contractions of isolated pig detrusor and vesical arterial smooth muscle: Calcium dependence and phosphoinositide hydrolysis" @default.
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• W2062912792 doi "https://doi.org/10.1016/0306-3623(92)90110-6" @default.
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