FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2062937767> ?p ?o ?g. }

• W2062937767 endingPage "e5643" @default.
• W2062937767 startingPage "e5643" @default.
• W2062937767 abstract "Current evidence suggests that endothelial progenitor cells (EPC) contribute to ischemic tissue repair by both secretion of paracrine factors and incorporation into developing vessels. We tested the hypothesis that cell-free administration of paracrine factors secreted by cultured EPC may achieve an angiogenic effect equivalent to cell therapy.EPC-derived conditioned medium (EPC-CM) was obtained from culture expanded EPC subjected to 72 hours of hypoxia. In vitro, EPC-CM significantly inhibited apoptosis of mature endothelial cells and promoted angiogenesis in a rat aortic ring assay. The therapeutic potential of EPC-CM as compared to EPC transplantation was evaluated in a rat model of chronic hindlimb ischemia. Serial intramuscular injections of EPC-CM and EPC both significantly increased hindlimb blood flow assessed by laser Doppler (81.2+/-2.9% and 83.7+/-3.0% vs. 53.5+/-2.4% of normal, P<0.01) and improved muscle performance. A significantly increased capillary density (1.62+/-0.03 and 1.68+/-0.05/muscle fiber, P<0.05), enhanced vascular maturation (8.6+/-0.3 and 8.1+/-0.4/HPF, P<0.05) and muscle viability corroborated the findings of improved hindlimb perfusion and muscle function. Furthermore, EPC-CM transplantation stimulated the mobilization of bone marrow (BM)-derived EPC compared to control (678.7+/-44.1 vs. 340.0+/-29.1 CD34(+)/CD45(-) cells/1x10(5) mononuclear cells, P<0.05) and their recruitment to the ischemic muscles (5.9+/-0.7 vs. 2.6+/-0.4 CD34(+) cells/HPF, P<0.001) 3 days after the last injection.Intramuscular injection of EPC-CM is as effective as cell transplantation for promoting tissue revascularization and functional recovery. Owing to the technical and practical limitations of cell therapy, cell free conditioned media may represent a potent alternative for therapeutic angiogenesis in ischemic cardiovascular diseases." @default.
• W2062937767 created "2016-06-24" @default.
• W2062937767 creator A5000085570 @default.
• W2062937767 creator A5023113049 @default.
• W2062937767 creator A5028268293 @default.
• W2062937767 creator A5037861132 @default.
• W2062937767 creator A5054604123 @default.
• W2062937767 creator A5082090719 @default.
• W2062937767 creator A5084489827 @default.
• W2062937767 date "2009-05-21" @default.
• W2062937767 modified "2023-10-14" @default.
• W2062937767 title "Novel Cell-Free Strategy for Therapeutic Angiogenesis: In Vitro Generated Conditioned Medium Can Replace Progenitor Cell Transplantation" @default.
• W2062937767 cites W1519008817 @default.
• W2062937767 cites W1544053957 @default.
• W2062937767 cites W1968495461 @default.
• W2062937767 cites W1969531944 @default.
• W2062937767 cites W1969937534 @default.
• W2062937767 cites W1971614601 @default.
• W2062937767 cites W1978238305 @default.
• W2062937767 cites W1978859651 @default.
• W2062937767 cites W1981096586 @default.
• W2062937767 cites W1981285537 @default.
• W2062937767 cites W1999579644 @default.
• W2062937767 cites W2005297392 @default.
• W2062937767 cites W2015789556 @default.
• W2062937767 cites W2022757974 @default.
• W2062937767 cites W2030008253 @default.
• W2062937767 cites W2038473729 @default.
• W2062937767 cites W2039409077 @default.
• W2062937767 cites W2045382887 @default.
• W2062937767 cites W2053706156 @default.
• W2062937767 cites W2054095354 @default.
• W2062937767 cites W2058195291 @default.
• W2062937767 cites W2060761913 @default.
• W2062937767 cites W2064238921 @default.
• W2062937767 cites W2064817281 @default.
• W2062937767 cites W2067072940 @default.
• W2062937767 cites W2069073230 @default.
• W2062937767 cites W2076896175 @default.
• W2062937767 cites W2080196370 @default.
• W2062937767 cites W2081678513 @default.
• W2062937767 cites W2093116780 @default.
• W2062937767 cites W2098444281 @default.
• W2062937767 cites W2098710781 @default.
• W2062937767 cites W2098743872 @default.
• W2062937767 cites W2099908568 @default.
• W2062937767 cites W2111947351 @default.
• W2062937767 cites W2112267683 @default.
• W2062937767 cites W2114222046 @default.
• W2062937767 cites W2116036875 @default.
• W2062937767 cites W2118995107 @default.
• W2062937767 cites W2124396531 @default.
• W2062937767 cites W2127296141 @default.
• W2062937767 cites W2127872440 @default.
• W2062937767 cites W2128553876 @default.
• W2062937767 cites W2130761693 @default.
• W2062937767 cites W2140003426 @default.
• W2062937767 cites W2144026946 @default.
• W2062937767 cites W2156148537 @default.
• W2062937767 cites W2157040346 @default.
• W2062937767 cites W2157603347 @default.
• W2062937767 cites W2157806433 @default.
• W2062937767 cites W2163087495 @default.
• W2062937767 cites W2165116711 @default.
• W2062937767 cites W2167595513 @default.
• W2062937767 cites W2171998177 @default.
• W2062937767 doi "https://doi.org/10.1371/journal.pone.0005643" @default.
• W2062937767 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2682571" @default.
• W2062937767 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19479066" @default.
• W2062937767 hasPublicationYear "2009" @default.
• W2062937767 type Work @default.
• W2062937767 sameAs 2062937767 @default.
• W2062937767 citedByCount "198" @default.
• W2062937767 countsByYear W20629377672012 @default.
• W2062937767 countsByYear W20629377672013 @default.
• W2062937767 countsByYear W20629377672014 @default.
• W2062937767 countsByYear W20629377672015 @default.
• W2062937767 countsByYear W20629377672016 @default.
• W2062937767 countsByYear W20629377672017 @default.
• W2062937767 countsByYear W20629377672018 @default.
• W2062937767 countsByYear W20629377672019 @default.
• W2062937767 countsByYear W20629377672020 @default.
• W2062937767 countsByYear W20629377672021 @default.
• W2062937767 countsByYear W20629377672022 @default.
• W2062937767 countsByYear W20629377672023 @default.
• W2062937767 crossrefType "journal-article" @default.
• W2062937767 hasAuthorship W2062937767A5000085570 @default.
• W2062937767 hasAuthorship W2062937767A5023113049 @default.
• W2062937767 hasAuthorship W2062937767A5028268293 @default.
• W2062937767 hasAuthorship W2062937767A5037861132 @default.
• W2062937767 hasAuthorship W2062937767A5054604123 @default.
• W2062937767 hasAuthorship W2062937767A5082090719 @default.
• W2062937767 hasAuthorship W2062937767A5084489827 @default.
• W2062937767 hasBestOaLocation W20629377671 @default.
• W2062937767 hasConcept C10205521 @default.
• W2062937767 hasConcept C10882517 @default.
• W2062937767 hasConcept C126322002 @default.
• W2062937767 hasConcept C134018914 @default.

• next