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• W2062953809 abstract "The gonadotropic regulation of granulosa cells steroidogenesis in vitro has been shown to be accompanied by cellular rounding. In this study, the possible relationship between cell shape, microtubules, and granulosa cell steroidogenesis in vitro was further explored by culturing (24 h) granulosa cells obtained from antral follicles of pregnant mare’s serum gonadotropin-treated rats in either Eagles’s Minimum Essential Medium alone (MEM-cells) or in collagen gels (GEL-cells) in the absence or presence of colchicine, a microtubule-depolymerizing agent previously shown to inhibit cell-spreading in vitro. Cellular morphology was assessed by electron microscopy and compared with that seen in vivo. In addition, the influence of the various culture conditions on progesterone and 20 α-hydroxy-pregn-4-en-3-one (20 α-OH-progesterone) secretion was determined by specific radioimmunoassays." @default.
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• W2062953809 date "1988-05-01" @default.
• W2062953809 modified "2023-09-24" @default.
• W2062953809 title "Culture of Granulosa Cells in Collagen Gels: The Influence of Cell Shape on Steroidogenesis1" @default.
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• W2062953809 doi "https://doi.org/10.1095/biolreprod38.4.881" @default.
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