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- W2063031552 abstract "Maturation was induced in Asterias oocytes with 1-methyladenine (1-MA) at a final concentration of 2 μM. At 5, 10, and 30 min of treatment, oocytes were homogenized and the cytosolic fraction was prepared. The cytosol was incubated with [γ-32P]ATP and [γ-32P]GTP. The phosphorylated proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the radioactivity in the gels was determined by autoradiography. The cytosol prepared from 1-MA-treated oocytes incubated with [γ-32P]ATP showed a marked increase in the radiolabeling of proteins with estimated molecular weights of 70,000 and 62,000 Da. With [γ-32P]GTP a 56,000-Da protein showed increased radiolabeling. The present finding suggests that an early biochemical event of 1-MA-induced oocyte maturation in Asterias is the stimulation of phosphorylation of specific proteins." @default.
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- W2063031552 date "1989-06-01" @default.
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- W2063031552 title "Protein phosphorylation during 1-methyladenine-induced maturation of Asterias oocytes" @default.
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- W2063031552 doi "https://doi.org/10.1016/0014-4827(89)90269-3" @default.
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