Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063057384> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2063057384 endingPage "4617" @default.
- W2063057384 startingPage "4611" @default.
- W2063057384 abstract "An adenosine cyclic 3',5'-monophosphate (cAMP) dependent protein kinase has recently been shown to exist in Dictyostelium discoideum and to be developmentally regulated. In this report we have followed the chromatographic behavior of both the holoenzyme and its subunits. A cAMP-dependent holoenzyme could be obtained from the 100000 g soluble fraction after passage through DE-52 cellulose (pH 7.5) and Sephacryl S300. Under conditions of low pH the holoenzyme could be further purified by flat-bed electrofocusing (pI = 6.8). Application of the holoenzyme to electrofocusing at high pH resulted in dissociation of the holoenzyme into a cAMP binding component (pI = 6.1) and a cAMP-independent catalytic activity (pI = 7.4). Dissociation of the holoenzyme into subunits also occurred during histone affinity chromatography and gel filtration chromatography (S300) in the presence of a dissociating buffer. Although the subunit structure was clearly evident during chromatography, the holoenzyme could not be dissociated by simple addition of cAMP to the extract. The catalytic subunit could be purified further by CM-Sephadex, DE-52 cellulose (pH 8.5), histone affinity, and hydrophobic chromatography. The regulatory subunit was further purified by DE-52 cellulose (pH 8.5) and cAMP affinity chromatography. Proof that the cAMP binding activity and the cAMP-independent catalytic activity were in fact the regulatory and catalytic subunits was shown by reconstitution of the cAMP-dependent holoenzyme from the purified subunits. By using these separation procedures, one can obtain from extracts of Dictyostelium the subunits that are free of each other as well as free of any endogenous protein substrates." @default.
- W2063057384 created "2016-06-24" @default.
- W2063057384 creator A5045437255 @default.
- W2063057384 creator A5052172146 @default.
- W2063057384 creator A5062699133 @default.
- W2063057384 creator A5082022679 @default.
- W2063057384 creator A5084057645 @default.
- W2063057384 date "1984-09-25" @default.
- W2063057384 modified "2023-10-14" @default.
- W2063057384 title "Chromatographic behavior of cyclic AMP-dependent protein kinase and its subunits from Dictyostelium discoideum" @default.
- W2063057384 cites W1543109110 @default.
- W2063057384 cites W1570227839 @default.
- W2063057384 cites W1598316265 @default.
- W2063057384 cites W1872210478 @default.
- W2063057384 cites W1973276488 @default.
- W2063057384 cites W1975210444 @default.
- W2063057384 cites W1990781232 @default.
- W2063057384 cites W1995945325 @default.
- W2063057384 cites W2012975616 @default.
- W2063057384 cites W2013686817 @default.
- W2063057384 cites W202988332 @default.
- W2063057384 cites W2031152435 @default.
- W2063057384 cites W2046886551 @default.
- W2063057384 cites W2057813337 @default.
- W2063057384 cites W2071495623 @default.
- W2063057384 cites W2076436621 @default.
- W2063057384 cites W2087575542 @default.
- W2063057384 cites W2088708307 @default.
- W2063057384 cites W2091367525 @default.
- W2063057384 cites W2094772812 @default.
- W2063057384 cites W2107453786 @default.
- W2063057384 cites W2140628532 @default.
- W2063057384 cites W2206884908 @default.
- W2063057384 cites W51133088 @default.
- W2063057384 doi "https://doi.org/10.1021/bi00315a015" @default.
- W2063057384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6093860" @default.
- W2063057384 hasPublicationYear "1984" @default.
- W2063057384 type Work @default.
- W2063057384 sameAs 2063057384 @default.
- W2063057384 citedByCount "14" @default.
- W2063057384 crossrefType "journal-article" @default.
- W2063057384 hasAuthorship W2063057384A5045437255 @default.
- W2063057384 hasAuthorship W2063057384A5052172146 @default.
- W2063057384 hasAuthorship W2063057384A5062699133 @default.
- W2063057384 hasAuthorship W2063057384A5082022679 @default.
- W2063057384 hasAuthorship W2063057384A5084057645 @default.
- W2063057384 hasConcept C104292427 @default.
- W2063057384 hasConcept C104317684 @default.
- W2063057384 hasConcept C179247698 @default.
- W2063057384 hasConcept C181199279 @default.
- W2063057384 hasConcept C184235292 @default.
- W2063057384 hasConcept C185592680 @default.
- W2063057384 hasConcept C193363816 @default.
- W2063057384 hasConcept C2775969001 @default.
- W2063057384 hasConcept C43617362 @default.
- W2063057384 hasConcept C55493867 @default.
- W2063057384 hasConcept C97029542 @default.
- W2063057384 hasConceptScore W2063057384C104292427 @default.
- W2063057384 hasConceptScore W2063057384C104317684 @default.
- W2063057384 hasConceptScore W2063057384C179247698 @default.
- W2063057384 hasConceptScore W2063057384C181199279 @default.
- W2063057384 hasConceptScore W2063057384C184235292 @default.
- W2063057384 hasConceptScore W2063057384C185592680 @default.
- W2063057384 hasConceptScore W2063057384C193363816 @default.
- W2063057384 hasConceptScore W2063057384C2775969001 @default.
- W2063057384 hasConceptScore W2063057384C43617362 @default.
- W2063057384 hasConceptScore W2063057384C55493867 @default.
- W2063057384 hasConceptScore W2063057384C97029542 @default.
- W2063057384 hasIssue "20" @default.
- W2063057384 hasLocation W20630573841 @default.
- W2063057384 hasLocation W20630573842 @default.
- W2063057384 hasOpenAccess W2063057384 @default.
- W2063057384 hasPrimaryLocation W20630573841 @default.
- W2063057384 hasRelatedWork W1971050671 @default.
- W2063057384 hasRelatedWork W1989918960 @default.
- W2063057384 hasRelatedWork W1993253042 @default.
- W2063057384 hasRelatedWork W2007203894 @default.
- W2063057384 hasRelatedWork W2007331329 @default.
- W2063057384 hasRelatedWork W2055000342 @default.
- W2063057384 hasRelatedWork W2063057384 @default.
- W2063057384 hasRelatedWork W2208401449 @default.
- W2063057384 hasRelatedWork W2461688035 @default.
- W2063057384 hasRelatedWork W44584688 @default.
- W2063057384 hasVolume "23" @default.
- W2063057384 isParatext "false" @default.
- W2063057384 isRetracted "false" @default.
- W2063057384 magId "2063057384" @default.
- W2063057384 workType "article" @default.