FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063058436> ?p ?o ?g. }

• W2063058436 endingPage "2870" @default.
• W2063058436 startingPage "2864" @default.
• W2063058436 abstract "Abstract Ankyrin repeats (AR) are 33‐residue motifs containing a β‐turn, followed by two α‐helices connected by a loop. AR occur in tandem arrangements and stack side‐by‐side to form elongated domains involved in very different cellular tasks. Recently, consensus libraries of AR repeats were constructed. Protein E1_5 represents a member of the shortest library, and consists of only a single consensus repeat flanked by designed N‐ and C‐terminal capping repeats. Here we present a biophysical characterization of this AR domain. The protein is compactly folded, as judged from the heat capacity of the native state and from the specific unfolding enthalpy and entropy. From spectroscopic data, thermal and urea‐induced unfolding can be modeled by a two‐state transition. However, scanning calorimetry experiments reveal a deviation from the two‐state behavior at elevated temperatures. Folding and unfolding at 5°C both follow monoexponential kinetics with k folding = 28 sec −1 and k unfolding = 0.9 sec −1 . Kinetic and equilibrium unfolding parameters at 5°C agree very well. We conclude that E1_5 folds in a simple two‐state manner at low temperatures while equilibrium intermediates become populated at higher temperatures. A chevron‐plot analysis indicates that the protein traverses a very compact transition state along the folding/unfolding pathway. This work demonstrates that a designed minimal ankyrin repeat protein has the thermodynamic and kinetic properties of a compactly folded protein, and explains the favorable properties of the consensus framework." @default.
• W2063058436 created "2016-06-24" @default.
• W2063058436 creator A5018159033 @default.
• W2063058436 creator A5027692708 @default.
• W2063058436 creator A5044017505 @default.
• W2063058436 creator A5063734176 @default.
• W2063058436 creator A5083658123 @default.
• W2063058436 creator A5090422381 @default.
• W2063058436 date "2004-11-01" @default.
• W2063058436 modified "2023-10-13" @default.
• W2063058436 title "Folding of a designed simple ankyrin repeat protein" @default.
• W2063058436 cites W1533569014 @default.
• W2063058436 cites W1964610252 @default.
• W2063058436 cites W1987392010 @default.
• W2063058436 cites W1989149568 @default.
• W2063058436 cites W1991443411 @default.
• W2063058436 cites W2002195659 @default.
• W2063058436 cites W2006501334 @default.
• W2063058436 cites W2019087056 @default.
• W2063058436 cites W2030221757 @default.
• W2063058436 cites W2037926176 @default.
• W2063058436 cites W2044682488 @default.
• W2063058436 cites W2045603413 @default.
• W2063058436 cites W2047970950 @default.
• W2063058436 cites W2049003834 @default.
• W2063058436 cites W2056455937 @default.
• W2063058436 cites W2060619683 @default.
• W2063058436 cites W2066688961 @default.
• W2063058436 cites W2080602313 @default.
• W2063058436 cites W2081848458 @default.
• W2063058436 cites W2084679948 @default.
• W2063058436 cites W2084938664 @default.
• W2063058436 cites W2092803887 @default.
• W2063058436 cites W2094799958 @default.
• W2063058436 cites W2097962012 @default.
• W2063058436 cites W2105888864 @default.
• W2063058436 cites W2109413613 @default.
• W2063058436 cites W2119586900 @default.
• W2063058436 cites W2148571760 @default.
• W2063058436 cites W2150608129 @default.
• W2063058436 cites W2151469241 @default.
• W2063058436 cites W2153724411 @default.
• W2063058436 cites W2161936455 @default.
• W2063058436 cites W2163181687 @default.
• W2063058436 cites W2168528671 @default.
• W2063058436 doi "https://doi.org/10.1110/ps.04935704" @default.
• W2063058436 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2286595" @default.
• W2063058436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15498935" @default.
• W2063058436 hasPublicationYear "2004" @default.
• W2063058436 type Work @default.
• W2063058436 sameAs 2063058436 @default.
• W2063058436 citedByCount "39" @default.
• W2063058436 countsByYear W20630584362012 @default.
• W2063058436 countsByYear W20630584362017 @default.
• W2063058436 countsByYear W20630584362018 @default.
• W2063058436 countsByYear W20630584362021 @default.
• W2063058436 countsByYear W20630584362023 @default.
• W2063058436 crossrefType "journal-article" @default.
• W2063058436 hasAuthorship W2063058436A5018159033 @default.
• W2063058436 hasAuthorship W2063058436A5027692708 @default.
• W2063058436 hasAuthorship W2063058436A5044017505 @default.
• W2063058436 hasAuthorship W2063058436A5063734176 @default.
• W2063058436 hasAuthorship W2063058436A5083658123 @default.
• W2063058436 hasAuthorship W2063058436A5090422381 @default.
• W2063058436 hasBestOaLocation W20630584361 @default.
• W2063058436 hasConcept C104317684 @default.
• W2063058436 hasConcept C113241181 @default.
• W2063058436 hasConcept C121332964 @default.
• W2063058436 hasConcept C12554922 @default.
• W2063058436 hasConcept C133571119 @default.
• W2063058436 hasConcept C141231307 @default.
• W2063058436 hasConcept C159985019 @default.
• W2063058436 hasConcept C175188612 @default.
• W2063058436 hasConcept C182800266 @default.
• W2063058436 hasConcept C185592680 @default.
• W2063058436 hasConcept C192562407 @default.
• W2063058436 hasConcept C19472624 @default.
• W2063058436 hasConcept C204328495 @default.
• W2063058436 hasConcept C206276672 @default.
• W2063058436 hasConcept C27149982 @default.
• W2063058436 hasConcept C2777814067 @default.
• W2063058436 hasConcept C55493867 @default.
• W2063058436 hasConcept C8010536 @default.
• W2063058436 hasConcept C86803240 @default.
• W2063058436 hasConcept C97355855 @default.
• W2063058436 hasConceptScore W2063058436C104317684 @default.
• W2063058436 hasConceptScore W2063058436C113241181 @default.
• W2063058436 hasConceptScore W2063058436C121332964 @default.
• W2063058436 hasConceptScore W2063058436C12554922 @default.
• W2063058436 hasConceptScore W2063058436C133571119 @default.
• W2063058436 hasConceptScore W2063058436C141231307 @default.
• W2063058436 hasConceptScore W2063058436C159985019 @default.
• W2063058436 hasConceptScore W2063058436C175188612 @default.
• W2063058436 hasConceptScore W2063058436C182800266 @default.
• W2063058436 hasConceptScore W2063058436C185592680 @default.
• W2063058436 hasConceptScore W2063058436C192562407 @default.
• W2063058436 hasConceptScore W2063058436C19472624 @default.
• W2063058436 hasConceptScore W2063058436C204328495 @default.

• next