Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063081254> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2063081254 endingPage "304" @default.
- W2063081254 startingPage "299" @default.
- W2063081254 abstract "Brachyspira pilosicoli is an anaerobic intestinal spirochaete that colonizes the large intestine of various host species, in which it may induce diarrhoea, poor growth rates and a localized colitis known as intestinal (or colonic) spirochaetosis. The spirochaete is considered to be potentially zoonotic. The purpose of the current study was to develop a multiple-locus variable number tandem repeat analysis (MLVA) method as a simple and rapid tool to investigate the molecular epidemiology of B. pilosicoli. The genomic sequence of B. pilosicoli strain 95/1000 was analyzed for potential tandem repeats using the default parameters of the Tandem Repeat Finder program. A total of 22 repeat loci were identified and tested for their presence and variability on a set of 10 B. pilosicoli isolates. Five loci that were present in most isolates and that showed evidence of allelic variation were selected and used with a collection of 119 isolates from different host species and geographical locations. Not all the isolates amplified at all loci, but using the available data a total of 103 VNTR profiles were generated. The discriminatory power of this method was 0.976. A phylogenetic tree constructed from the allelic profiles confirmed the diversity of B. pilosicoli, and the general lack of clustering of strains based on species of origin or geographic origin. Some isolates with known epidemiological links were found to be identical or highly similar. The MLVA method was simple and easy to use, and could readily differentiate between strains of B. pilosicoli. MLVA should prove to be a useful tool for rapid identification of relationships between B. pilosicoli isolates in epidemiological investigations." @default.
- W2063081254 created "2016-06-24" @default.
- W2063081254 creator A5004050862 @default.
- W2063081254 creator A5042228573 @default.
- W2063081254 creator A5053469584 @default.
- W2063081254 creator A5065858202 @default.
- W2063081254 date "2013-05-01" @default.
- W2063081254 modified "2023-09-27" @default.
- W2063081254 title "Multiple locus variable number tandem repeat analysis (MLVA) of the pathogenic intestinal spirochaete Brachyspira pilosicoli" @default.
- W2063081254 cites W1792185329 @default.
- W2063081254 cites W1968979737 @default.
- W2063081254 cites W1975585714 @default.
- W2063081254 cites W1984702361 @default.
- W2063081254 cites W1986252877 @default.
- W2063081254 cites W1995805750 @default.
- W2063081254 cites W2036294724 @default.
- W2063081254 cites W2040742249 @default.
- W2063081254 cites W2049027350 @default.
- W2063081254 cites W2051702142 @default.
- W2063081254 cites W2069406487 @default.
- W2063081254 cites W2081154679 @default.
- W2063081254 cites W2083145206 @default.
- W2063081254 cites W2110470553 @default.
- W2063081254 cites W2112814753 @default.
- W2063081254 cites W2113960960 @default.
- W2063081254 cites W2124897848 @default.
- W2063081254 cites W2143860051 @default.
- W2063081254 cites W2146181562 @default.
- W2063081254 cites W2168186226 @default.
- W2063081254 cites W2169325493 @default.
- W2063081254 cites W2147666688 @default.
- W2063081254 doi "https://doi.org/10.1016/j.vetmic.2012.12.030" @default.
- W2063081254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23391437" @default.
- W2063081254 hasPublicationYear "2013" @default.
- W2063081254 type Work @default.
- W2063081254 sameAs 2063081254 @default.
- W2063081254 citedByCount "7" @default.
- W2063081254 countsByYear W20630812542013 @default.
- W2063081254 countsByYear W20630812542014 @default.
- W2063081254 countsByYear W20630812542017 @default.
- W2063081254 countsByYear W20630812542018 @default.
- W2063081254 countsByYear W20630812542019 @default.
- W2063081254 countsByYear W20630812542020 @default.
- W2063081254 crossrefType "journal-article" @default.
- W2063081254 hasAuthorship W2063081254A5004050862 @default.
- W2063081254 hasAuthorship W2063081254A5042228573 @default.
- W2063081254 hasAuthorship W2063081254A5053469584 @default.
- W2063081254 hasAuthorship W2063081254A5065858202 @default.
- W2063081254 hasBestOaLocation W20630812542 @default.
- W2063081254 hasConcept C104317684 @default.
- W2063081254 hasConcept C141231307 @default.
- W2063081254 hasConcept C180754005 @default.
- W2063081254 hasConcept C189036908 @default.
- W2063081254 hasConcept C193252679 @default.
- W2063081254 hasConcept C27149982 @default.
- W2063081254 hasConcept C2777264056 @default.
- W2063081254 hasConcept C28245876 @default.
- W2063081254 hasConcept C523546767 @default.
- W2063081254 hasConcept C54355233 @default.
- W2063081254 hasConcept C84597430 @default.
- W2063081254 hasConcept C86803240 @default.
- W2063081254 hasConcept C89423630 @default.
- W2063081254 hasConceptScore W2063081254C104317684 @default.
- W2063081254 hasConceptScore W2063081254C141231307 @default.
- W2063081254 hasConceptScore W2063081254C180754005 @default.
- W2063081254 hasConceptScore W2063081254C189036908 @default.
- W2063081254 hasConceptScore W2063081254C193252679 @default.
- W2063081254 hasConceptScore W2063081254C27149982 @default.
- W2063081254 hasConceptScore W2063081254C2777264056 @default.
- W2063081254 hasConceptScore W2063081254C28245876 @default.
- W2063081254 hasConceptScore W2063081254C523546767 @default.
- W2063081254 hasConceptScore W2063081254C54355233 @default.
- W2063081254 hasConceptScore W2063081254C84597430 @default.
- W2063081254 hasConceptScore W2063081254C86803240 @default.
- W2063081254 hasConceptScore W2063081254C89423630 @default.
- W2063081254 hasIssue "3-4" @default.
- W2063081254 hasLocation W20630812541 @default.
- W2063081254 hasLocation W20630812542 @default.
- W2063081254 hasLocation W20630812543 @default.
- W2063081254 hasOpenAccess W2063081254 @default.
- W2063081254 hasPrimaryLocation W20630812541 @default.
- W2063081254 hasRelatedWork W127700499 @default.
- W2063081254 hasRelatedWork W1969143901 @default.
- W2063081254 hasRelatedWork W2042464734 @default.
- W2063081254 hasRelatedWork W2050126397 @default.
- W2063081254 hasRelatedWork W2057739827 @default.
- W2063081254 hasRelatedWork W2062856551 @default.
- W2063081254 hasRelatedWork W2063081254 @default.
- W2063081254 hasRelatedWork W2416657901 @default.
- W2063081254 hasRelatedWork W2565408376 @default.
- W2063081254 hasRelatedWork W290807016 @default.
- W2063081254 hasVolume "163" @default.
- W2063081254 isParatext "false" @default.
- W2063081254 isRetracted "false" @default.
- W2063081254 magId "2063081254" @default.
- W2063081254 workType "article" @default.