FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063119992> ?p ?o ?g. }

• W2063119992 endingPage "157" @default.
• W2063119992 startingPage "148" @default.
• W2063119992 abstract "Abstract Background Fluorescence resonance energy transfer applied in flow cytometry (FCET) is an excellent tool for determining supramolecular organization of biomolecules at the cell surface or inside the cell. Availability of new fluorophores and cytometers requires the establishment of fluorophore dye pairs most suitable for FCET measurements. Methods A gastric tumor cell line (N87) was labeled for major histocompatibility complex class I heavy chain and β2‐microglobulin with antibodies conjugated with fluorescein‐ and indocarbocyanine‐like fluorophores and analyzed in FCET measurements on a cell‐by‐cell basis using three flow cytometers: FACSCalibur, FACSDiVa, and FACSArray. Results Normalized fluorescence intensity values were measured and normalized energy transfer efficiencies, spectral overlap integrals, and crucial dye‐ and instrument‐dependent parameters were calculated for all matching pairs of seven fluorophores on the three commercial cytometers. The most crucial parameter in determining the applicability of the donor‐acceptor pairs was the normalized fluorescence intensity and the least important one was the spectral overlap. Conclusions On the basis of available laser lines, the optimal dye pair for all three cytometers is the Alexa546‐Alexa647 pair, which produces high energy transfer efficiency values and has the best spectral characteristics with regard to laser excitation, detection of emission, and spectral overlap. © 2005 Wiley‐Liss, Inc." @default.
• W2063119992 created "2016-06-24" @default.
• W2063119992 creator A5003803175 @default.
• W2063119992 creator A5015486088 @default.
• W2063119992 creator A5016844824 @default.
• W2063119992 creator A5027677198 @default.
• W2063119992 creator A5056919449 @default.
• W2063119992 creator A5078449638 @default.
• W2063119992 creator A5082945980 @default.
• W2063119992 date "2005-05-10" @default.
• W2063119992 modified "2023-10-18" @default.
• W2063119992 title "Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements" @default.
• W2063119992 cites W1490295882 @default.
• W2063119992 cites W1525689409 @default.
• W2063119992 cites W1601889378 @default.
• W2063119992 cites W1605766765 @default.
• W2063119992 cites W1616564451 @default.
• W2063119992 cites W1963621346 @default.
• W2063119992 cites W1965797967 @default.
• W2063119992 cites W1972710675 @default.
• W2063119992 cites W1973474908 @default.
• W2063119992 cites W1979488473 @default.
• W2063119992 cites W1982675645 @default.
• W2063119992 cites W1983053645 @default.
• W2063119992 cites W1991190990 @default.
• W2063119992 cites W1999697754 @default.
• W2063119992 cites W2025307506 @default.
• W2063119992 cites W2030024386 @default.
• W2063119992 cites W2030355540 @default.
• W2063119992 cites W2037104161 @default.
• W2063119992 cites W2041743929 @default.
• W2063119992 cites W2046682570 @default.
• W2063119992 cites W2056743609 @default.
• W2063119992 cites W2062485439 @default.
• W2063119992 cites W2066304950 @default.
• W2063119992 cites W2068281228 @default.
• W2063119992 cites W2068764377 @default.
• W2063119992 cites W2086070797 @default.
• W2063119992 cites W2094517427 @default.
• W2063119992 cites W2102557954 @default.
• W2063119992 cites W2107483408 @default.
• W2063119992 cites W2121981369 @default.
• W2063119992 cites W2131970139 @default.
• W2063119992 cites W2151991230 @default.
• W2063119992 cites W2155308169 @default.
• W2063119992 cites W2156293240 @default.
• W2063119992 cites W2159203587 @default.
• W2063119992 cites W2169278628 @default.
• W2063119992 cites W2476651486 @default.
• W2063119992 cites W4230350617 @default.
• W2063119992 doi "https://doi.org/10.1002/cyto.a.20142" @default.
• W2063119992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15825180" @default.
• W2063119992 hasPublicationYear "2005" @default.
• W2063119992 type Work @default.
• W2063119992 sameAs 2063119992 @default.
• W2063119992 citedByCount "42" @default.
• W2063119992 countsByYear W20631199922012 @default.
• W2063119992 countsByYear W20631199922013 @default.
• W2063119992 countsByYear W20631199922014 @default.
• W2063119992 countsByYear W20631199922015 @default.
• W2063119992 countsByYear W20631199922016 @default.
• W2063119992 countsByYear W20631199922017 @default.
• W2063119992 countsByYear W20631199922019 @default.
• W2063119992 countsByYear W20631199922023 @default.
• W2063119992 crossrefType "journal-article" @default.
• W2063119992 hasAuthorship W2063119992A5003803175 @default.
• W2063119992 hasAuthorship W2063119992A5015486088 @default.
• W2063119992 hasAuthorship W2063119992A5016844824 @default.
• W2063119992 hasAuthorship W2063119992A5027677198 @default.
• W2063119992 hasAuthorship W2063119992A5056919449 @default.
• W2063119992 hasAuthorship W2063119992A5078449638 @default.
• W2063119992 hasAuthorship W2063119992A5082945980 @default.
• W2063119992 hasConcept C104317684 @default.
• W2063119992 hasConcept C120665830 @default.
• W2063119992 hasConcept C121332964 @default.
• W2063119992 hasConcept C142613039 @default.
• W2063119992 hasConcept C185592680 @default.
• W2063119992 hasConcept C186060115 @default.
• W2063119992 hasConcept C192562407 @default.
• W2063119992 hasConcept C2778015030 @default.
• W2063119992 hasConcept C2778617687 @default.
• W2063119992 hasConcept C2779260011 @default.
• W2063119992 hasConcept C520434653 @default.
• W2063119992 hasConcept C55493867 @default.
• W2063119992 hasConcept C86803240 @default.
• W2063119992 hasConcept C91881484 @default.
• W2063119992 hasConcept C96305047 @default.
• W2063119992 hasConceptScore W2063119992C104317684 @default.
• W2063119992 hasConceptScore W2063119992C120665830 @default.
• W2063119992 hasConceptScore W2063119992C121332964 @default.
• W2063119992 hasConceptScore W2063119992C142613039 @default.
• W2063119992 hasConceptScore W2063119992C185592680 @default.
• W2063119992 hasConceptScore W2063119992C186060115 @default.
• W2063119992 hasConceptScore W2063119992C192562407 @default.
• W2063119992 hasConceptScore W2063119992C2778015030 @default.
• W2063119992 hasConceptScore W2063119992C2778617687 @default.
• W2063119992 hasConceptScore W2063119992C2779260011 @default.
• W2063119992 hasConceptScore W2063119992C520434653 @default.

• next