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- W2063240025 abstract "We developed a novel electrochemiluminescence (ECL) ethanol biosensor based on Ru(bpy)32+ and alcohol dehydrogenase (ADH) immobilized by graphene/bovine serum albumin composite film. The graphene film was directly formed on a glassy carbon electrode surface via an in situ reduction of graphene oxide (GO) and Ru(bpy)32+ was immobilized during its formation. The graphene film acted as both a decorating agent for immobilization of Ru(bpy)32+ and a matrix to immobilize bovine serum albumin (BSA), meanwhile BSA not only acted as a reductant to reduce GO, but also provided a friendly environment for ADH immobilization. Furthermore, ADH was separated from Ru(bpy)32+ by the electron-conductive graphene/BSA composite film to retain its enzymatic activity. The experimental results indicated that the biosensor had excellent electrochemical activity, ECL response to ethanol and stability. Such a design of Ru(bpy)32+–graphene/BSA film to modify electrode holds a great promise as a new biocompatible platform for the development of enzyme-based ECL biosensors." @default.
- W2063240025 created "2016-06-24" @default.
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- W2063240025 date "2013-03-01" @default.
- W2063240025 modified "2023-10-17" @default.
- W2063240025 title "A novel electrochemiluminescence ethanol biosensor based on tris(2,2′-bipyridine) ruthenium (II) and alcohol dehydrogenase immobilized in graphene/bovine serum albumin composite film" @default.
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- W2063240025 doi "https://doi.org/10.1016/j.bios.2012.10.005" @default.
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