Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063261061> ?p ?o ?g. }
- W2063261061 endingPage "282" @default.
- W2063261061 startingPage "275" @default.
- W2063261061 abstract "Mutations in COL1A1 or COL1A2 genes lead to osteogenesis Imperfecta (OI) in humans. There are three possiblities to successfully treat OI including (1) gene therapy, (2) mesenchymal stem cell (MSC) therapy, or (3) a combination of both. The aim of this study was to develop a model for combined gene/cell OI therapy by targeting Col1a1 and Col1a2 genes with isogenic sequences from corresponding human genes in rat bone marrow (BM)-derived MSCs. The recombination efficacy was tested for five different rat-human-rat hybrid DNAs with rat fragments that were 1 to 4 kb long. For selection of transfected clones a neomycine resistance gene was cotransfected, and clones resistant to G418 (G418(+)) were recovered and screened for integration of specific gene loci in the rat genome. Over 90% of G418(+) clones correctly integrated the rat-human-rat hybrid DNAs, and both OI loci in the rat genome were targeted to a similar degree. Longer homologous sequences integrated into rat collagen genes approximately 10 times more efficiently. Based on our data the nonviral gene targeting technology could be potentially employed to repair collagen genes in OI patients." @default.
- W2063261061 created "2016-06-24" @default.
- W2063261061 creator A5006391574 @default.
- W2063261061 creator A5014536606 @default.
- W2063261061 creator A5015531924 @default.
- W2063261061 creator A5032107825 @default.
- W2063261061 creator A5037181676 @default.
- W2063261061 creator A5060529151 @default.
- W2063261061 creator A5061490386 @default.
- W2063261061 creator A5082678963 @default.
- W2063261061 date "2010-06-01" @default.
- W2063261061 modified "2023-10-06" @default.
- W2063261061 title "Optimization of Genetic Engineering and Homologous Recombination of Collagen Type I Genes in Rat Bone Marrow Mesenchymal Stem Cells (MSC)" @default.
- W2063261061 cites W1534731743 @default.
- W2063261061 cites W1965953554 @default.
- W2063261061 cites W1967241081 @default.
- W2063261061 cites W1968084001 @default.
- W2063261061 cites W1978173381 @default.
- W2063261061 cites W1991453189 @default.
- W2063261061 cites W1995869907 @default.
- W2063261061 cites W2001627351 @default.
- W2063261061 cites W2002179012 @default.
- W2063261061 cites W2039849855 @default.
- W2063261061 cites W2050060496 @default.
- W2063261061 cites W2053895193 @default.
- W2063261061 cites W2054521968 @default.
- W2063261061 cites W2061750887 @default.
- W2063261061 cites W2061981400 @default.
- W2063261061 cites W2068102253 @default.
- W2063261061 cites W2070014707 @default.
- W2063261061 cites W2072863928 @default.
- W2063261061 cites W2074846276 @default.
- W2063261061 cites W2080169614 @default.
- W2063261061 cites W2086274688 @default.
- W2063261061 cites W2090214004 @default.
- W2063261061 cites W2098447041 @default.
- W2063261061 cites W2102627115 @default.
- W2063261061 cites W2103964277 @default.
- W2063261061 cites W2104978512 @default.
- W2063261061 cites W2105677787 @default.
- W2063261061 cites W2138977668 @default.
- W2063261061 cites W2140748527 @default.
- W2063261061 cites W2146274306 @default.
- W2063261061 cites W2154974204 @default.
- W2063261061 cites W2159999899 @default.
- W2063261061 cites W2167575455 @default.
- W2063261061 cites W2168031723 @default.
- W2063261061 cites W2170976321 @default.
- W2063261061 doi "https://doi.org/10.1089/cell.2009.0084" @default.
- W2063261061 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20698769" @default.
- W2063261061 hasPublicationYear "2010" @default.
- W2063261061 type Work @default.
- W2063261061 sameAs 2063261061 @default.
- W2063261061 citedByCount "10" @default.
- W2063261061 countsByYear W20632610612012 @default.
- W2063261061 countsByYear W20632610612013 @default.
- W2063261061 countsByYear W20632610612014 @default.
- W2063261061 countsByYear W20632610612021 @default.
- W2063261061 countsByYear W20632610612023 @default.
- W2063261061 crossrefType "journal-article" @default.
- W2063261061 hasAuthorship W2063261061A5006391574 @default.
- W2063261061 hasAuthorship W2063261061A5014536606 @default.
- W2063261061 hasAuthorship W2063261061A5015531924 @default.
- W2063261061 hasAuthorship W2063261061A5032107825 @default.
- W2063261061 hasAuthorship W2063261061A5037181676 @default.
- W2063261061 hasAuthorship W2063261061A5060529151 @default.
- W2063261061 hasAuthorship W2063261061A5061490386 @default.
- W2063261061 hasAuthorship W2063261061A5082678963 @default.
- W2063261061 hasConcept C102744134 @default.
- W2063261061 hasConcept C104317684 @default.
- W2063261061 hasConcept C111599444 @default.
- W2063261061 hasConcept C111829913 @default.
- W2063261061 hasConcept C141231307 @default.
- W2063261061 hasConcept C153911025 @default.
- W2063261061 hasConcept C198826908 @default.
- W2063261061 hasConcept C203014093 @default.
- W2063261061 hasConcept C2780007613 @default.
- W2063261061 hasConcept C54009773 @default.
- W2063261061 hasConcept C54355233 @default.
- W2063261061 hasConcept C64894306 @default.
- W2063261061 hasConcept C86803240 @default.
- W2063261061 hasConceptScore W2063261061C102744134 @default.
- W2063261061 hasConceptScore W2063261061C104317684 @default.
- W2063261061 hasConceptScore W2063261061C111599444 @default.
- W2063261061 hasConceptScore W2063261061C111829913 @default.
- W2063261061 hasConceptScore W2063261061C141231307 @default.
- W2063261061 hasConceptScore W2063261061C153911025 @default.
- W2063261061 hasConceptScore W2063261061C198826908 @default.
- W2063261061 hasConceptScore W2063261061C203014093 @default.
- W2063261061 hasConceptScore W2063261061C2780007613 @default.
- W2063261061 hasConceptScore W2063261061C54009773 @default.
- W2063261061 hasConceptScore W2063261061C54355233 @default.
- W2063261061 hasConceptScore W2063261061C64894306 @default.
- W2063261061 hasConceptScore W2063261061C86803240 @default.
- W2063261061 hasIssue "3" @default.
- W2063261061 hasLocation W20632610611 @default.
- W2063261061 hasLocation W20632610612 @default.
- W2063261061 hasOpenAccess W2063261061 @default.