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• W2063272636 abstract "Although recombinase polymerase amplification (RPA) has many advantages for the detection of pathogenic nucleic acids in point-of-care applications, RPA has not yet been implemented to quantify sample concentration using a standard curve. Here, we describe a real-time RPA assay with an internal positive control and an algorithm that analyzes real-time fluorescence data to quantify HIV-1 DNA. We show that DNA concentration and the onset of detectable amplification are correlated by an exponential standard curve. In a set of experiments in which the standard curve and algorithm were used to analyze and quantify additional DNA samples, the algorithm predicted an average concentration within 1 order of magnitude of the correct concentration for all HIV-1 DNA concentrations tested. These results suggest that quantitative RPA (qRPA) may serve as a powerful tool for quantifying nucleic acids and may be adapted for use in single-sample point-of-care diagnostic systems." @default.
• W2063272636 created "2016-06-24" @default.
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• W2063272636 date "2014-05-29" @default.
• W2063272636 modified "2023-10-10" @default.
• W2063272636 title "Quantification of HIV-1 DNA Using Real-Time Recombinase Polymerase Amplification" @default.
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• W2063272636 doi "https://doi.org/10.1021/ac5011298" @default.
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