FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063314715> ?p ?o ?g. }

• W2063314715 endingPage "861" @default.
• W2063314715 startingPage "852" @default.
• W2063314715 abstract "<h3>AIMS</h3> To investigate the distribution, persistence, and stability of fluorescently labelled phosphorothioate oligonucleotides (PS-ODNs) in normal and laser photocoagulated retina following intravitreal injection in the rat. <h3>METHODS</h3> Fluorescently labelled PS-ODNs were injected intravitreally into pigmented eyes at doses of 0.5–10.0 nmol in 2.0 μl solution. The dynamics of PS-ODNs was evaluated by fluorescent microscopy of cryosections and flat mounted retinal pigment epithelium (RPE)-choroid-sclera. Genescan analysis was used to assess the integrity of PS-ODNs in the retina after injection. The dynamics of PS-ODNs was also evaluated in the retina following krypton laser photocoagulation with a protocol producing choroidal neovascularisation (CNV). <h3>RESULTS</h3> Following intravitreal injection the PS-ODNs demonstrated dose and time dependent distribution and persistence in the retina, where they accessed all neural layers. However, they preferentially accumulated in the RPE layer, demonstrated as bright granules in the cytoplasm of the cells. Injections of 5.0 and 7.5 nmol of PS-ODNs exhibited strong fluorescence in the retina for 6 weeks after injection. Genescan analysis demonstrated that the PS-ODNs remained almost completely intact for at least 12 weeks. Following laser treatment, the PS-ODNs were concentrated in the regions of laser photocoagulation and retained high intensity for at least 8 weeks after injection, particularly localised to macrophages, RPE, and the local choroidal tissue. <h3>CONCLUSIONS</h3> These results indicate that PS-ODNs are stable and accessible to most neural layers of the retina, and they preferentially accumulate in the RPE layer following intravitreal injection. The successful delivery of PS-ODNs into normal and laser photocoagulated retina suggests that PS-ODNs may have potential in the development of therapy for attenuating retinal degenerations and CNV." @default.
• W2063314715 created "2016-06-24" @default.
• W2063314715 creator A5026650278 @default.
• W2063314715 creator A5042298251 @default.
• W2063314715 creator A5076085346 @default.
• W2063314715 creator A5084916536 @default.
• W2063314715 creator A5091241054 @default.
• W2063314715 date "1999-07-01" @default.
• W2063314715 modified "2023-09-25" @default.
• W2063314715 title "Dynamics of phosphorothioate oligonucleotides in normal and laser photocoagulated retina" @default.
• W2063314715 cites W1552951689 @default.
• W2063314715 cites W1561961789 @default.
• W2063314715 cites W1577823339 @default.
• W2063314715 cites W1886996198 @default.
• W2063314715 cites W1944646771 @default.
• W2063314715 cites W1966037701 @default.
• W2063314715 cites W1972773808 @default.
• W2063314715 cites W1975353048 @default.
• W2063314715 cites W1977760393 @default.
• W2063314715 cites W1981427921 @default.
• W2063314715 cites W1985737377 @default.
• W2063314715 cites W1999414884 @default.
• W2063314715 cites W2018586410 @default.
• W2063314715 cites W2019958880 @default.
• W2063314715 cites W2020678086 @default.
• W2063314715 cites W2022973264 @default.
• W2063314715 cites W2036385369 @default.
• W2063314715 cites W2037724847 @default.
• W2063314715 cites W2040844874 @default.
• W2063314715 cites W2050014764 @default.
• W2063314715 cites W2051842051 @default.
• W2063314715 cites W2055927905 @default.
• W2063314715 cites W2059873595 @default.
• W2063314715 cites W2065990347 @default.
• W2063314715 cites W2066768866 @default.
• W2063314715 cites W2079073589 @default.
• W2063314715 cites W2087218262 @default.
• W2063314715 cites W2088852187 @default.
• W2063314715 cites W2104032689 @default.
• W2063314715 cites W2112215500 @default.
• W2063314715 cites W2112694631 @default.
• W2063314715 cites W2153312133 @default.
• W2063314715 cites W2156322641 @default.
• W2063314715 cites W2158058021 @default.
• W2063314715 cites W2171791693 @default.
• W2063314715 cites W2181479067 @default.
• W2063314715 cites W2322167664 @default.
• W2063314715 cites W2322318138 @default.
• W2063314715 cites W2323909850 @default.
• W2063314715 cites W2399319395 @default.
• W2063314715 cites W2470555411 @default.
• W2063314715 cites W4211236610 @default.
• W2063314715 cites W45416255 @default.
• W2063314715 cites W90782486 @default.
• W2063314715 doi "https://doi.org/10.1136/bjo.83.7.852" @default.
• W2063314715 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1723113" @default.
• W2063314715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10381674" @default.
• W2063314715 hasPublicationYear "1999" @default.
• W2063314715 type Work @default.
• W2063314715 sameAs 2063314715 @default.
• W2063314715 citedByCount "17" @default.
• W2063314715 countsByYear W20633147152012 @default.
• W2063314715 countsByYear W20633147152013 @default.
• W2063314715 countsByYear W20633147152014 @default.
• W2063314715 countsByYear W20633147152015 @default.
• W2063314715 countsByYear W20633147152018 @default.
• W2063314715 crossrefType "journal-article" @default.
• W2063314715 hasAuthorship W2063314715A5026650278 @default.
• W2063314715 hasAuthorship W2063314715A5042298251 @default.
• W2063314715 hasAuthorship W2063314715A5076085346 @default.
• W2063314715 hasAuthorship W2063314715A5084916536 @default.
• W2063314715 hasAuthorship W2063314715A5091241054 @default.
• W2063314715 hasBestOaLocation W20633147151 @default.
• W2063314715 hasConcept C105702510 @default.
• W2063314715 hasConcept C118487528 @default.
• W2063314715 hasConcept C120665830 @default.
• W2063314715 hasConcept C121332964 @default.
• W2063314715 hasConcept C142724271 @default.
• W2063314715 hasConcept C169760540 @default.
• W2063314715 hasConcept C185592680 @default.
• W2063314715 hasConcept C2776694393 @default.
• W2063314715 hasConcept C2777093970 @default.
• W2063314715 hasConcept C2777100477 @default.
• W2063314715 hasConcept C2778024200 @default.
• W2063314715 hasConcept C2780827179 @default.
• W2063314715 hasConcept C520434653 @default.
• W2063314715 hasConcept C71924100 @default.
• W2063314715 hasConcept C86803240 @default.
• W2063314715 hasConcept C91881484 @default.
• W2063314715 hasConceptScore W2063314715C105702510 @default.
• W2063314715 hasConceptScore W2063314715C118487528 @default.
• W2063314715 hasConceptScore W2063314715C120665830 @default.
• W2063314715 hasConceptScore W2063314715C121332964 @default.
• W2063314715 hasConceptScore W2063314715C142724271 @default.
• W2063314715 hasConceptScore W2063314715C169760540 @default.
• W2063314715 hasConceptScore W2063314715C185592680 @default.
• W2063314715 hasConceptScore W2063314715C2776694393 @default.
• W2063314715 hasConceptScore W2063314715C2777093970 @default.

• next