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• W2063346711 abstract "Quantitative fluorescence-based methods have been developed to determine the nuclear concentration of polyamide–fluorescein conjugates in cell culture. Confocal laser scanning microscopy and flow cytometry techniques are utilized to plot calibration curves, from which the nuclear concentration can be interpolated. Upon treatment with polyamide, the concentration in the nucleus of live HeLa cells is calculated to be between 0.1 and 0.5 μM, which is significantly lower than the 2 μM dosage concentration. In contrast, the observed nuclear concentration in U251 cells is closer to the dosage concentration, indicating a cell line-specific increase in uptake for this class of compounds. Although confocal microscopy and flow cytometry generate disparate values, taken together these experiments suggest that the polyamide concentration inside the cell nucleus is lower than it is outside the cell." @default.
• W2063346711 created "2016-06-24" @default.
• W2063346711 creator A5053299010 @default.
• W2063346711 creator A5076578241 @default.
• W2063346711 date "2008-11-01" @default.
• W2063346711 modified "2023-10-17" @default.
• W2063346711 title "Quantitating the concentration of Py-Im polyamide–fluorescein conjugates in live cells" @default.
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• W2063346711 doi "https://doi.org/10.1016/j.bmcl.2008.05.063" @default.
• W2063346711 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2587135" @default.
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