Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063366087> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2063366087 endingPage "1602" @default.
- W2063366087 startingPage "1593" @default.
- W2063366087 abstract "An improved chemical method, capable of derivatizing all natural amino acids to their corresponding thiohydantoins, is described. This involves activation by acetyl chloride in TFA followed by derivatization with ammonium thiocyanate. Possible interference of reactive side chains was investigated by reacting N-acetylamino acids as well as several peptides with propionyl chloride instead of acetyl chloride. The products were characterized by PDMS mass spectrometry and 1H-NMR. This chemical method allows, for the first time, complete derivatization of N-acetylproline to proline thiohydantoin. Applying this chemistry to peptides with a C-terminal proline, the yields for formation of proline thiohydantoin were found to be up to 60%, depending on the peptide sequence. The previous inability to derivatize C-terminal proline to thiohydantoin was thought to stem from the fact that proline cannot form the oxazolonium ion required for efficient reaction with the thiocyanate ion. However, we have found mass spectrometric evidence for the existence of a proline oxazolonium ion, under basic as well as under acidic conditions. This improvement in derivatization of C-terminal amino acids including proline is a major step forward in the development of a general chemical C-terminal sequencing method that permits the C-terminal sequence analysis of proteins of any amino acid composition." @default.
- W2063366087 created "2016-06-24" @default.
- W2063366087 creator A5031672684 @default.
- W2063366087 creator A5039470983 @default.
- W2063366087 creator A5042730297 @default.
- W2063366087 creator A5074392120 @default.
- W2063366087 date "1998-07-01" @default.
- W2063366087 modified "2023-09-27" @default.
- W2063366087 title "An improved chemical approach toward the C-terminal sequence analysis of proteins containing all natural amino acids" @default.
- W2063366087 cites W125383525 @default.
- W2063366087 cites W1277189608 @default.
- W2063366087 cites W1569459811 @default.
- W2063366087 cites W1964775984 @default.
- W2063366087 cites W1965233796 @default.
- W2063366087 cites W1972129395 @default.
- W2063366087 cites W1974985416 @default.
- W2063366087 cites W1980160494 @default.
- W2063366087 cites W1993261140 @default.
- W2063366087 cites W2001529123 @default.
- W2063366087 cites W2005793881 @default.
- W2063366087 cites W2007824546 @default.
- W2063366087 cites W2018932932 @default.
- W2063366087 cites W2022338986 @default.
- W2063366087 cites W2024484384 @default.
- W2063366087 cites W2032447451 @default.
- W2063366087 cites W2054221027 @default.
- W2063366087 cites W2055089554 @default.
- W2063366087 cites W2058966102 @default.
- W2063366087 cites W2059193805 @default.
- W2063366087 cites W2064614623 @default.
- W2063366087 cites W2084240312 @default.
- W2063366087 cites W2089146019 @default.
- W2063366087 cites W2089611548 @default.
- W2063366087 cites W2089755851 @default.
- W2063366087 cites W2321864594 @default.
- W2063366087 cites W2322031411 @default.
- W2063366087 cites W2322508358 @default.
- W2063366087 cites W2323198271 @default.
- W2063366087 cites W2332732579 @default.
- W2063366087 doi "https://doi.org/10.1002/pro.5560070713" @default.
- W2063366087 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2144064" @default.
- W2063366087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9684892" @default.
- W2063366087 hasPublicationYear "1998" @default.
- W2063366087 type Work @default.
- W2063366087 sameAs 2063366087 @default.
- W2063366087 citedByCount "12" @default.
- W2063366087 countsByYear W20633660872013 @default.
- W2063366087 crossrefType "journal-article" @default.
- W2063366087 hasAuthorship W2063366087A5031672684 @default.
- W2063366087 hasAuthorship W2063366087A5039470983 @default.
- W2063366087 hasAuthorship W2063366087A5042730297 @default.
- W2063366087 hasAuthorship W2063366087A5074392120 @default.
- W2063366087 hasBestOaLocation W20633660872 @default.
- W2063366087 hasConcept C162356407 @default.
- W2063366087 hasConcept C178790620 @default.
- W2063366087 hasConcept C185592680 @default.
- W2063366087 hasConcept C2776923573 @default.
- W2063366087 hasConcept C2778695967 @default.
- W2063366087 hasConcept C2779815552 @default.
- W2063366087 hasConcept C43617362 @default.
- W2063366087 hasConcept C515207424 @default.
- W2063366087 hasConcept C55493867 @default.
- W2063366087 hasConceptScore W2063366087C162356407 @default.
- W2063366087 hasConceptScore W2063366087C178790620 @default.
- W2063366087 hasConceptScore W2063366087C185592680 @default.
- W2063366087 hasConceptScore W2063366087C2776923573 @default.
- W2063366087 hasConceptScore W2063366087C2778695967 @default.
- W2063366087 hasConceptScore W2063366087C2779815552 @default.
- W2063366087 hasConceptScore W2063366087C43617362 @default.
- W2063366087 hasConceptScore W2063366087C515207424 @default.
- W2063366087 hasConceptScore W2063366087C55493867 @default.
- W2063366087 hasIssue "7" @default.
- W2063366087 hasLocation W20633660871 @default.
- W2063366087 hasLocation W20633660872 @default.
- W2063366087 hasLocation W20633660873 @default.
- W2063366087 hasLocation W20633660874 @default.
- W2063366087 hasOpenAccess W2063366087 @default.
- W2063366087 hasPrimaryLocation W20633660871 @default.
- W2063366087 hasRelatedWork W1983661517 @default.
- W2063366087 hasRelatedWork W1987886540 @default.
- W2063366087 hasRelatedWork W2009475667 @default.
- W2063366087 hasRelatedWork W2065377794 @default.
- W2063366087 hasRelatedWork W2073733503 @default.
- W2063366087 hasRelatedWork W2086107997 @default.
- W2063366087 hasRelatedWork W2115218143 @default.
- W2063366087 hasRelatedWork W2149906371 @default.
- W2063366087 hasRelatedWork W2365534081 @default.
- W2063366087 hasRelatedWork W2916126509 @default.
- W2063366087 hasVolume "7" @default.
- W2063366087 isParatext "false" @default.
- W2063366087 isRetracted "false" @default.
- W2063366087 magId "2063366087" @default.
- W2063366087 workType "article" @default.