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• W2063387941 abstract "Entamoeba histolytica is susceptible to complement attack in its lumen-dwelling state and develops complement resistance during pathogenic tissue invasion. As experimental evidence suggests that this change in phenotype is accompanied by a change in gene expression, we constructed a subtractive cDNA library to identify genes involved. Poly(A) + RNA from complement-sensitive trophozoites was subtracted from single stranded cDNA derived from complement-resistant ones. Transcripts enriched in the library were found to code for a putative polypeptide comprising all sequence elements characteristic for serine/threonine protein kinases. The gene contains an intron of 46 nucleotides and two polyadenylation sites. Northern-blot analyses confirmed that the gene is expressed in both tissue-derived and laboratory-grown forms of complement-resistant E. histolytica." @default.
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• W2063387941 date "1996-10-01" @default.
• W2063387941 modified "2023-10-03" @default.
• W2063387941 title "Putative serine/threonine protein kinase expressed in complement-resistant forms of Entamoeba histolytica" @default.
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• W2063387941 doi "https://doi.org/10.1016/0166-6851(96)02684-9" @default.
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