Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063389275> ?p ?o ?g. }
- W2063389275 endingPage "176" @default.
- W2063389275 startingPage "167" @default.
- W2063389275 abstract "Glomerular hypertrophy and hyperfiltration are the two major pathological characteristics of the early stages of diabetic nephropathy (DN), which are respectively related to mesangial cell (MC) proliferation and a decrease in calcium influx conducted by canonical transient receptor potential cation channel 6 (TRPC6). The marked increase in the production of reactive oxygen species (ROS) induced by hyperglycemia is the main sponsor of multiple pathological pathways in DN. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is an important source of ROS production in MCs. Astragaloside IV (AS‑IV) is an active ingredient of Radix Astragali which has a potent antioxidative effect. In this study, we aimed to investigate whether high glucose (HG)‑induced NADPH oxidase activation and ROS production contribute to MC proliferation and the downregulation of TRPC6 expression; we also wished to determine the effects of AS‑IV on MCs under HG conditions. Using a human glomerular mesangial cell line, we found that treatment with AS‑IV for 48 h markedly attenuated HG‑induced proliferation and the hypertrophy of MCs in a dose‑dependent manner. The intracellular ROS level was also markedly reduced following treatment with AS‑IV. In addition, the enhanced activity of NADPH oxidase and the expression level of NADPH oxidase 4 (Nox4) protein were decreased. Treatment with AS‑IV also inhibited the phosphorylation level of Akt and IκBα in the MCs. In addition, TRPC6 protein expression and the intracellular free calcium concentration were also markedly reduced following treatment with AS‑IV under HG conditions. These results suggest that AS‑IV inhibits HG‑induced mesangial cell proliferation and glomerular contractile dysfunction through the NADPH oxidase/ROS/Akt/nuclear factor‑κB (NF‑κB) pathway, providing a new perspective for the clinical treatment of DN." @default.
- W2063389275 created "2016-06-24" @default.
- W2063389275 creator A5011875487 @default.
- W2063389275 creator A5041135293 @default.
- W2063389275 creator A5048292232 @default.
- W2063389275 creator A5060667885 @default.
- W2063389275 creator A5074953539 @default.
- W2063389275 date "2014-04-09" @default.
- W2063389275 modified "2023-10-16" @default.
- W2063389275 title "Astragaloside IV prevents damage to human mesangial cells through the inhibition of the NADPH oxidase/ROS/Akt/NF-κB pathway under high glucose conditions" @default.
- W2063389275 cites W1968648927 @default.
- W2063389275 cites W1969901996 @default.
- W2063389275 cites W1970132914 @default.
- W2063389275 cites W1972634148 @default.
- W2063389275 cites W1977424733 @default.
- W2063389275 cites W1984683131 @default.
- W2063389275 cites W1987172857 @default.
- W2063389275 cites W1995996138 @default.
- W2063389275 cites W1996193998 @default.
- W2063389275 cites W1998454166 @default.
- W2063389275 cites W2002892625 @default.
- W2063389275 cites W2011279501 @default.
- W2063389275 cites W2019063477 @default.
- W2063389275 cites W2020763506 @default.
- W2063389275 cites W2023102331 @default.
- W2063389275 cites W2027393096 @default.
- W2063389275 cites W2031920038 @default.
- W2063389275 cites W2036801917 @default.
- W2063389275 cites W2042069668 @default.
- W2063389275 cites W2051687712 @default.
- W2063389275 cites W2055801973 @default.
- W2063389275 cites W2057160041 @default.
- W2063389275 cites W2058105565 @default.
- W2063389275 cites W2066000649 @default.
- W2063389275 cites W2081482296 @default.
- W2063389275 cites W2088547091 @default.
- W2063389275 cites W2089156332 @default.
- W2063389275 cites W2091352240 @default.
- W2063389275 cites W2095825179 @default.
- W2063389275 cites W2099925178 @default.
- W2063389275 cites W2108810370 @default.
- W2063389275 cites W2110913974 @default.
- W2063389275 cites W2127065370 @default.
- W2063389275 cites W2127162150 @default.
- W2063389275 cites W2129150771 @default.
- W2063389275 cites W2130931490 @default.
- W2063389275 cites W2133678286 @default.
- W2063389275 cites W2141688177 @default.
- W2063389275 cites W2142693924 @default.
- W2063389275 cites W2144593405 @default.
- W2063389275 cites W2146266459 @default.
- W2063389275 cites W2153571985 @default.
- W2063389275 cites W2157174760 @default.
- W2063389275 cites W2168438265 @default.
- W2063389275 cites W2340264230 @default.
- W2063389275 cites W4233547545 @default.
- W2063389275 cites W4242182607 @default.
- W2063389275 doi "https://doi.org/10.3892/ijmm.2014.1741" @default.
- W2063389275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24718766" @default.
- W2063389275 hasPublicationYear "2014" @default.
- W2063389275 type Work @default.
- W2063389275 sameAs 2063389275 @default.
- W2063389275 citedByCount "56" @default.
- W2063389275 countsByYear W20633892752015 @default.
- W2063389275 countsByYear W20633892752016 @default.
- W2063389275 countsByYear W20633892752017 @default.
- W2063389275 countsByYear W20633892752018 @default.
- W2063389275 countsByYear W20633892752019 @default.
- W2063389275 countsByYear W20633892752020 @default.
- W2063389275 countsByYear W20633892752021 @default.
- W2063389275 countsByYear W20633892752022 @default.
- W2063389275 countsByYear W20633892752023 @default.
- W2063389275 crossrefType "journal-article" @default.
- W2063389275 hasAuthorship W2063389275A5011875487 @default.
- W2063389275 hasAuthorship W2063389275A5041135293 @default.
- W2063389275 hasAuthorship W2063389275A5048292232 @default.
- W2063389275 hasAuthorship W2063389275A5060667885 @default.
- W2063389275 hasAuthorship W2063389275A5074953539 @default.
- W2063389275 hasBestOaLocation W20633892751 @default.
- W2063389275 hasConcept C126322002 @default.
- W2063389275 hasConcept C134018914 @default.
- W2063389275 hasConcept C181199279 @default.
- W2063389275 hasConcept C185592680 @default.
- W2063389275 hasConcept C190283241 @default.
- W2063389275 hasConcept C202751555 @default.
- W2063389275 hasConcept C2777209548 @default.
- W2063389275 hasConcept C2777989768 @default.
- W2063389275 hasConcept C2779131090 @default.
- W2063389275 hasConcept C2779719074 @default.
- W2063389275 hasConcept C2779765511 @default.
- W2063389275 hasConcept C2781308076 @default.
- W2063389275 hasConcept C38485361 @default.
- W2063389275 hasConcept C48349386 @default.
- W2063389275 hasConcept C55493867 @default.
- W2063389275 hasConcept C62112901 @default.
- W2063389275 hasConcept C71924100 @default.
- W2063389275 hasConcept C75217442 @default.
- W2063389275 hasConcept C86803240 @default.