Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063390247> ?p ?o ?g. }
- W2063390247 endingPage "131" @default.
- W2063390247 startingPage "122" @default.
- W2063390247 abstract "In this work a sequential multiplex PCR system was designed and validated for the detection of most frequent foodborne pathogen Vibrio species in fish and seafood (Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginoliticus and Vibrio mimicus). The method proposed functions in a hierarchical way, being composed of an end-point multiplex PCR to detect the presence of DNA belonging to the studied species, followed by multiplex PCR and fragment analysis allowing the viability assessment of the detected strains. The final multiplex PCR step of the method may be applied if identification of the serogroup, biotype and/or virulence factor level is necessary. Forty samples of commercial fish and seafood products were used at the method validation stage. Sixty three marine organism samples obtained from various estuarine areas of Spain including shrimps, crabs, bivalve mollusks and fishes were screened for presence of Vibrio species and 2 mussel samples were found positive for V. parahaemolyticus. On the whole, the proposed method is robust and readily adaptable in routine molecular diagnostic laboratories, allowing monitoring and simultaneous detection of all these bacterial pathogens in seafood samples, reducing the expenses and time consumed by other analytical methods." @default.
- W2063390247 created "2016-06-24" @default.
- W2063390247 creator A5008448122 @default.
- W2063390247 creator A5021078139 @default.
- W2063390247 creator A5067035515 @default.
- W2063390247 creator A5074953687 @default.
- W2063390247 date "2010-02-01" @default.
- W2063390247 modified "2023-10-13" @default.
- W2063390247 title "Validation of a method for the detection of five species, serogroups, biotypes and virulence factors of Vibrio by multiplex PCR in fish and seafood" @default.
- W2063390247 cites W1528021356 @default.
- W2063390247 cites W1894869549 @default.
- W2063390247 cites W1901842060 @default.
- W2063390247 cites W1924050422 @default.
- W2063390247 cites W1969246318 @default.
- W2063390247 cites W1971952038 @default.
- W2063390247 cites W1990265872 @default.
- W2063390247 cites W1990747884 @default.
- W2063390247 cites W2005579909 @default.
- W2063390247 cites W2013717530 @default.
- W2063390247 cites W2021615560 @default.
- W2063390247 cites W2021660632 @default.
- W2063390247 cites W2028886067 @default.
- W2063390247 cites W2030116644 @default.
- W2063390247 cites W2040078921 @default.
- W2063390247 cites W2049480490 @default.
- W2063390247 cites W2055930696 @default.
- W2063390247 cites W2062374917 @default.
- W2063390247 cites W2071199752 @default.
- W2063390247 cites W2076071631 @default.
- W2063390247 cites W2076855649 @default.
- W2063390247 cites W2077806252 @default.
- W2063390247 cites W2079489537 @default.
- W2063390247 cites W2086178060 @default.
- W2063390247 cites W2098864243 @default.
- W2063390247 cites W2119470061 @default.
- W2063390247 cites W2146237557 @default.
- W2063390247 cites W2231981240 @default.
- W2063390247 doi "https://doi.org/10.1016/j.fm.2009.09.004" @default.
- W2063390247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19913702" @default.
- W2063390247 hasPublicationYear "2010" @default.
- W2063390247 type Work @default.
- W2063390247 sameAs 2063390247 @default.
- W2063390247 citedByCount "40" @default.
- W2063390247 countsByYear W20633902472012 @default.
- W2063390247 countsByYear W20633902472013 @default.
- W2063390247 countsByYear W20633902472014 @default.
- W2063390247 countsByYear W20633902472015 @default.
- W2063390247 countsByYear W20633902472016 @default.
- W2063390247 countsByYear W20633902472017 @default.
- W2063390247 countsByYear W20633902472018 @default.
- W2063390247 countsByYear W20633902472019 @default.
- W2063390247 countsByYear W20633902472020 @default.
- W2063390247 countsByYear W20633902472021 @default.
- W2063390247 countsByYear W20633902472022 @default.
- W2063390247 crossrefType "journal-article" @default.
- W2063390247 hasAuthorship W2063390247A5008448122 @default.
- W2063390247 hasAuthorship W2063390247A5021078139 @default.
- W2063390247 hasAuthorship W2063390247A5067035515 @default.
- W2063390247 hasAuthorship W2063390247A5074953687 @default.
- W2063390247 hasConcept C104317684 @default.
- W2063390247 hasConcept C158278297 @default.
- W2063390247 hasConcept C2776154503 @default.
- W2063390247 hasConcept C2776460866 @default.
- W2063390247 hasConcept C2776575720 @default.
- W2063390247 hasConcept C2778458500 @default.
- W2063390247 hasConcept C2778643871 @default.
- W2063390247 hasConcept C2781028344 @default.
- W2063390247 hasConcept C2781188995 @default.
- W2063390247 hasConcept C2909208804 @default.
- W2063390247 hasConcept C49105822 @default.
- W2063390247 hasConcept C505870484 @default.
- W2063390247 hasConcept C523546767 @default.
- W2063390247 hasConcept C54355233 @default.
- W2063390247 hasConcept C60987743 @default.
- W2063390247 hasConcept C86803240 @default.
- W2063390247 hasConcept C89423630 @default.
- W2063390247 hasConcept C90583042 @default.
- W2063390247 hasConceptScore W2063390247C104317684 @default.
- W2063390247 hasConceptScore W2063390247C158278297 @default.
- W2063390247 hasConceptScore W2063390247C2776154503 @default.
- W2063390247 hasConceptScore W2063390247C2776460866 @default.
- W2063390247 hasConceptScore W2063390247C2776575720 @default.
- W2063390247 hasConceptScore W2063390247C2778458500 @default.
- W2063390247 hasConceptScore W2063390247C2778643871 @default.
- W2063390247 hasConceptScore W2063390247C2781028344 @default.
- W2063390247 hasConceptScore W2063390247C2781188995 @default.
- W2063390247 hasConceptScore W2063390247C2909208804 @default.
- W2063390247 hasConceptScore W2063390247C49105822 @default.
- W2063390247 hasConceptScore W2063390247C505870484 @default.
- W2063390247 hasConceptScore W2063390247C523546767 @default.
- W2063390247 hasConceptScore W2063390247C54355233 @default.
- W2063390247 hasConceptScore W2063390247C60987743 @default.
- W2063390247 hasConceptScore W2063390247C86803240 @default.
- W2063390247 hasConceptScore W2063390247C89423630 @default.
- W2063390247 hasConceptScore W2063390247C90583042 @default.
- W2063390247 hasIssue "1" @default.
- W2063390247 hasLocation W20633902471 @default.
- W2063390247 hasLocation W20633902472 @default.