FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063397275> ?p ?o ?g. }

• W2063397275 endingPage "240" @default.
• W2063397275 startingPage "231" @default.
• W2063397275 abstract "In contrast to other P450 enzymes purified from rat liver microsomes, purified P450 IIIA1 (P450p) is catalytically inactive when reconstituted with NADPH-cytochrome P450 reductase and the synthetic lipid, dilauroylphosphatidylcholine. However, purified P450 IIIA1 catalyzes the oxidation of testosterone when reconstituted with NADPH-cytochrome P450 reductase, cytochrome b5, an extract of microsomal lipid, and detergent (Emulgen 911). The present study demonstrates that the microsomal lipid extract can be replaced with one of several naturally occurring phospholipids, but not with cholesterol, sphingosine, sphingomyelin, ceramide, cerebroside, or cardiolipin. The ratio of the testosterone metabolites formed by purified P450 IIIA1 (i.e., 2 beta-, 6 beta-, and 15 beta-hydroxytestosterone) was influenced by the type of phospholipid added to the reconstitution system. The ability to replace microsomal lipid extract with several different phospholipids suggests that the nature of the polar group (i.e., choline, serine, ethanolamine, or inositol) is not critical for P450 IIIA1 activity, which implies that P450 IIIA1 activity is highly dependent on the fatty acid component of these lipids. To test this possibility, P450 IIIA1 was reconstituted with a series of synthetic phosphatidylcholines. Those phosphatidylcholines containing saturated fatty acids were unable to support testosterone oxidation by purified P450 IIIA1, regardless of the acyl chain length (C6 to C18). In contrast, several unsaturated phosphatidylcholines supported testosterone oxidation by purified P450 IIIA1, and in this regard dioleoylphosphatidylcholine (PC(18:1)2) was as effective as microsomal lipid extract and naturally occurring phosphatidylcholine or phosphatidylserine. These results confirmed that P450 IIIA1 activity is highly dependent on the fatty acid component of phospholipids. A second series of experiments was undertaken to determine whether microsomal P450 IIIA1, like the purified enzyme, is dependent on cytochrome b5. A polyclonal antibody against purified cytochrome b5 was raised in rabbits and was purified by affinity chromatography. Anti-cytochrome b5 caused a approximately 60% inhibition of testosterone 2 beta-, 6 beta-, and 15 beta-hydroxylation by purified P450 IIIA1 and inhibited these same reactions by approximately 70% when added to liver microsomes from dexamethasone-induced female rats. Overall, these results suggest that testosterone oxidation by microsomal cytochrome P450 IIIA1 requires cytochrome b5 and phospholipid containing unsaturated fatty acids." @default.
• W2063397275 created "2016-06-24" @default.
• W2063397275 creator A5038814197 @default.
• W2063397275 creator A5089059452 @default.
• W2063397275 date "1991-12-01" @default.
• W2063397275 modified "2023-10-10" @default.
• W2063397275 title "Cytochrome P450 IIIA1 (P450p) requires cytochrome b5 and phospholipid with unsaturated fatty acids" @default.
• W2063397275 cites W1234052980 @default.
• W2063397275 cites W1426742482 @default.
• W2063397275 cites W1500814526 @default.
• W2063397275 cites W1504804986 @default.
• W2063397275 cites W1509553808 @default.
• W2063397275 cites W1520965071 @default.
• W2063397275 cites W1527380199 @default.
• W2063397275 cites W1532212858 @default.
• W2063397275 cites W1540602405 @default.
• W2063397275 cites W1563928419 @default.
• W2063397275 cites W1588794217 @default.
• W2063397275 cites W1590889826 @default.
• W2063397275 cites W1608334798 @default.
• W2063397275 cites W1700470441 @default.
• W2063397275 cites W1885736358 @default.
• W2063397275 cites W1892479606 @default.
• W2063397275 cites W1984437618 @default.
• W2063397275 cites W1990731317 @default.
• W2063397275 cites W1993019185 @default.
• W2063397275 cites W2010701693 @default.
• W2063397275 cites W2018951949 @default.
• W2063397275 cites W2020721696 @default.
• W2063397275 cites W2021022873 @default.
• W2063397275 cites W2021900417 @default.
• W2063397275 cites W2032177410 @default.
• W2063397275 cites W2034877438 @default.
• W2063397275 cites W2044695596 @default.
• W2063397275 cites W2044702794 @default.
• W2063397275 cites W2048342700 @default.
• W2063397275 cites W2052428509 @default.
• W2063397275 cites W2053060655 @default.
• W2063397275 cites W2057406230 @default.
• W2063397275 cites W2059499685 @default.
• W2063397275 cites W2062474863 @default.
• W2063397275 cites W2063518072 @default.
• W2063397275 cites W2065516217 @default.
• W2063397275 cites W2072669931 @default.
• W2063397275 cites W2074731939 @default.
• W2063397275 cites W2075200935 @default.
• W2063397275 cites W2084191427 @default.
• W2063397275 cites W2098008357 @default.
• W2063397275 cites W2120944108 @default.
• W2063397275 cites W2139508000 @default.
• W2063397275 cites W2151201559 @default.
• W2063397275 cites W2164646340 @default.
• W2063397275 cites W2168526937 @default.
• W2063397275 cites W2341636685 @default.
• W2063397275 cites W3955559 @default.
• W2063397275 doi "https://doi.org/10.1016/0003-9861(91)90128-6" @default.
• W2063397275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1659320" @default.
• W2063397275 hasPublicationYear "1991" @default.
• W2063397275 type Work @default.
• W2063397275 sameAs 2063397275 @default.
• W2063397275 citedByCount "35" @default.
• W2063397275 countsByYear W20633972752014 @default.
• W2063397275 countsByYear W20633972752015 @default.
• W2063397275 countsByYear W20633972752017 @default.
• W2063397275 countsByYear W20633972752023 @default.
• W2063397275 crossrefType "journal-article" @default.
• W2063397275 hasAuthorship W2063397275A5038814197 @default.
• W2063397275 hasAuthorship W2063397275A5089059452 @default.
• W2063397275 hasConcept C181199279 @default.
• W2063397275 hasConcept C185592680 @default.
• W2063397275 hasConcept C190283241 @default.
• W2063397275 hasConcept C2775970874 @default.
• W2063397275 hasConcept C2776330855 @default.
• W2063397275 hasConcept C2776909261 @default.
• W2063397275 hasConcept C2777851122 @default.
• W2063397275 hasConcept C2778163477 @default.
• W2063397275 hasConcept C2778918659 @default.
• W2063397275 hasConcept C2779384962 @default.
• W2063397275 hasConcept C2781302388 @default.
• W2063397275 hasConcept C41625074 @default.
• W2063397275 hasConcept C526171541 @default.
• W2063397275 hasConcept C543025807 @default.
• W2063397275 hasConcept C55493867 @default.
• W2063397275 hasConcept C61322309 @default.
• W2063397275 hasConcept C87644729 @default.
• W2063397275 hasConceptScore W2063397275C181199279 @default.
• W2063397275 hasConceptScore W2063397275C185592680 @default.
• W2063397275 hasConceptScore W2063397275C190283241 @default.
• W2063397275 hasConceptScore W2063397275C2775970874 @default.
• W2063397275 hasConceptScore W2063397275C2776330855 @default.
• W2063397275 hasConceptScore W2063397275C2776909261 @default.
• W2063397275 hasConceptScore W2063397275C2777851122 @default.
• W2063397275 hasConceptScore W2063397275C2778163477 @default.
• W2063397275 hasConceptScore W2063397275C2778918659 @default.
• W2063397275 hasConceptScore W2063397275C2779384962 @default.
• W2063397275 hasConceptScore W2063397275C2781302388 @default.
• W2063397275 hasConceptScore W2063397275C41625074 @default.
• W2063397275 hasConceptScore W2063397275C526171541 @default.

• next