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• W2063422963 abstract "β -Tricalcium phosphate ( β -TCP) micron particles whose diameters range from 1 μ m to 10 μ m have been recently developed, however, their biological effects remain unknown. We investigated the biological effects of β -TCP micron particles on proliferation, cytotoxicity, and calcification of human synovial mesenchymal stem cells (MSCs). MSCs were cultured without dexamethasone, β -glycerophosphate, or ascorbic acid. 1.0 mg/mL β -TCP micron particles inhibited proliferation of MSCs significantly and increased dead cells. In the contact condition, 0.1 mg/mL β -TCP micron particles promoted calcification of MSCs evaluated by alizarin red staining and enhanced mRNA expressions of runx2, osteopontin, and type I collagen. In the noncontact condition, these effects were not observed. 0.1 mg/mL β -TCP micron particles increased calcium concentration in the medium in the contact condition, while 1.0 mg/mL β -TCP micron particles decreased calcium and phosphorus concentrations in the medium in the noncontact condition. By transmission electron microscopy, β -TCP micron particles were localized in the phagosome of MSCs and were dissolved. In conclusion, β -TCP micron particles promoted calcification of MSCs and enhanced osteogenesis-related gene expressions in vitro ." @default.
• W2063422963 created "2016-06-24" @default.
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• W2063422963 date "2013-01-01" @default.
• W2063422963 modified "2023-10-06" @default.
• W2063422963 title "<i>β</i>-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells<i>In Vitro</i>" @default.
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• W2063422963 doi "https://doi.org/10.1155/2013/426786" @default.
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