FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063452922> ?p ?o ?g. }

• W2063452922 endingPage "557" @default.
• W2063452922 startingPage "553" @default.
• W2063452922 abstract "The amino acid sequence (1–301aa) coding the human PTP1B catalytic domain (PTP1Bc) was obtained from the GenBank. The PTP1Bc gene was constructed by overlapping PCR, then inserted into vector pET-22b (+) and expressed efficiently in E. coli BL21 (DE3) under the optimization condition after IPTG induction. The proteins were expressed mainly as inclusion bodies with the yield of more than 30% of total bacterial proteins. The expressed products were purified through Ni 2+ -affinity chromatographic column. After purification, the purity of the proteins was more than 95%. The result of western blotting confirmed that the purified proteins could specially combine with anti-PTP1B antibody. The enzyme activities experiment showed that the protein had phosphatase activities. The gene construction, expression, and purification of PTP1Bc may provide basis for further study of its functions." @default.
• W2063452922 created "2016-06-24" @default.
• W2063452922 creator A5008825393 @default.
• W2063452922 creator A5032944465 @default.
• W2063452922 creator A5040260157 @default.
• W2063452922 creator A5057007670 @default.
• W2063452922 creator A5078479753 @default.
• W2063452922 date "2008-04-01" @default.
• W2063452922 modified "2023-09-24" @default.
• W2063452922 title "Expression and Activity Analysis of the Catalytic Domain of PTP1B" @default.
• W2063452922 cites W1497274503 @default.
• W2063452922 cites W1499420960 @default.
• W2063452922 cites W1992936971 @default.
• W2063452922 cites W2008528817 @default.
• W2063452922 cites W2031763777 @default.
• W2063452922 cites W2036708427 @default.
• W2063452922 cites W2056028442 @default.
• W2063452922 cites W2064298888 @default.
• W2063452922 cites W2080175465 @default.
• W2063452922 cites W2092819259 @default.
• W2063452922 cites W2145501578 @default.
• W2063452922 cites W2355893987 @default.
• W2063452922 doi "https://doi.org/10.1016/s1872-2075(08)60026-6" @default.
• W2063452922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18616161" @default.
• W2063452922 hasPublicationYear "2008" @default.
• W2063452922 type Work @default.
• W2063452922 sameAs 2063452922 @default.
• W2063452922 citedByCount "4" @default.
• W2063452922 countsByYear W20634529222012 @default.
• W2063452922 countsByYear W20634529222019 @default.
• W2063452922 countsByYear W20634529222021 @default.
• W2063452922 crossrefType "journal-article" @default.
• W2063452922 hasAuthorship W2063452922A5008825393 @default.
• W2063452922 hasAuthorship W2063452922A5032944465 @default.
• W2063452922 hasAuthorship W2063452922A5040260157 @default.
• W2063452922 hasAuthorship W2063452922A5057007670 @default.
• W2063452922 hasAuthorship W2063452922A5078479753 @default.
• W2063452922 hasConcept C104317684 @default.
• W2063452922 hasConcept C153911025 @default.
• W2063452922 hasConcept C170344550 @default.
• W2063452922 hasConcept C179247698 @default.
• W2063452922 hasConcept C181199279 @default.
• W2063452922 hasConcept C185592680 @default.
• W2063452922 hasConcept C203750385 @default.
• W2063452922 hasConcept C2780430158 @default.
• W2063452922 hasConcept C33161422 @default.
• W2063452922 hasConcept C40767141 @default.
• W2063452922 hasConcept C515207424 @default.
• W2063452922 hasConcept C55493867 @default.
• W2063452922 hasConcept C79029880 @default.
• W2063452922 hasConcept C85265743 @default.
• W2063452922 hasConcept C86803240 @default.
• W2063452922 hasConcept C8985478 @default.
• W2063452922 hasConcept C91779695 @default.
• W2063452922 hasConceptScore W2063452922C104317684 @default.
• W2063452922 hasConceptScore W2063452922C153911025 @default.
• W2063452922 hasConceptScore W2063452922C170344550 @default.
• W2063452922 hasConceptScore W2063452922C179247698 @default.
• W2063452922 hasConceptScore W2063452922C181199279 @default.
• W2063452922 hasConceptScore W2063452922C185592680 @default.
• W2063452922 hasConceptScore W2063452922C203750385 @default.
• W2063452922 hasConceptScore W2063452922C2780430158 @default.
• W2063452922 hasConceptScore W2063452922C33161422 @default.
• W2063452922 hasConceptScore W2063452922C40767141 @default.
• W2063452922 hasConceptScore W2063452922C515207424 @default.
• W2063452922 hasConceptScore W2063452922C55493867 @default.
• W2063452922 hasConceptScore W2063452922C79029880 @default.
• W2063452922 hasConceptScore W2063452922C85265743 @default.
• W2063452922 hasConceptScore W2063452922C86803240 @default.
• W2063452922 hasConceptScore W2063452922C8985478 @default.
• W2063452922 hasConceptScore W2063452922C91779695 @default.
• W2063452922 hasIssue "4" @default.
• W2063452922 hasLocation W20634529221 @default.
• W2063452922 hasLocation W20634529222 @default.
• W2063452922 hasOpenAccess W2063452922 @default.
• W2063452922 hasPrimaryLocation W20634529221 @default.
• W2063452922 hasRelatedWork W139522596 @default.
• W2063452922 hasRelatedWork W2188620046 @default.
• W2063452922 hasRelatedWork W2275798607 @default.
• W2063452922 hasRelatedWork W2353772236 @default.
• W2063452922 hasRelatedWork W2360454982 @default.
• W2063452922 hasRelatedWork W2360803140 @default.
• W2063452922 hasRelatedWork W2363306255 @default.
• W2063452922 hasRelatedWork W2367682826 @default.
• W2063452922 hasRelatedWork W2372785093 @default.
• W2063452922 hasRelatedWork W2381137905 @default.
• W2063452922 hasRelatedWork W2384393807 @default.
• W2063452922 hasRelatedWork W2386653738 @default.
• W2063452922 hasRelatedWork W2389338035 @default.
• W2063452922 hasRelatedWork W2390072996 @default.
• W2063452922 hasRelatedWork W2390962370 @default.
• W2063452922 hasRelatedWork W2392071501 @default.
• W2063452922 hasRelatedWork W2395420635 @default.
• W2063452922 hasRelatedWork W2746217503 @default.
• W2063452922 hasRelatedWork W2518514275 @default.
• W2063452922 hasRelatedWork W3030461245 @default.
• W2063452922 hasVolume "24" @default.
• W2063452922 isParatext "false" @default.

• next