Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063485138> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2063485138 endingPage "664" @default.
- W2063485138 startingPage "657" @default.
- W2063485138 abstract "Occupancy of chemotactic peptide receptors leads to rapid initiation of phospholipase D (PLD) activity in intact dimethylsulfoxide-differentiated HL-60 granulocytes (Pai, J.-K, Siegel, M.I., Egan, R.W., and Billah, M.M. (1988) J. Biol. Chem. 263, 12472). To gain further insight into the activation mechanisms, PLD has been studied in cell lysates from HL-60 granulocytes, using 1-0-alkyl-2-oleoyl-[32P]phosphatidylcholine (alkyl-[32P]PC), 1-0-[3H]alkyl-2-oleoyl-phosphatidylcholine ([3H]alkyl-PC) and [14C]arachidonyl-phospholipids as substrates. In the presence of Ca2+ and GTPγS, post-nuclear homogenates degrade alkyl-[32P]PC to produce 1-0-alkyl-[32P]phosphatidic acid (alkyl-[32P]-PA), and in the presence of ethanol, also 1-0-alkyl-[32P]phosphatidylethanol (alkyl-[32P]PEt). By comparing the 3H/32P ratios of PA and PEt to that of PC, it is concluded that PA and PEt are formed exclusively by a PLD that catalyzes both hydrolysis and transphosphatidylation between PC and ethanol. Furthermore, PC containing either ester- or ether-linkage at the sn-1 position is degraded in preference to phosphatidylethanolamine and phosphatidylinositol by PLD in HL-60 cell homogenates. It is concluded that HL-60 granulocytes contain a PC-specific PLD that requires both Ca2+ and GTP for activation." @default.
- W2063485138 created "2016-06-24" @default.
- W2063485138 creator A5005757827 @default.
- W2063485138 creator A5024512000 @default.
- W2063485138 creator A5046002593 @default.
- W2063485138 creator A5046195597 @default.
- W2063485138 creator A5049562626 @default.
- W2063485138 date "1989-08-01" @default.
- W2063485138 modified "2023-09-26" @default.
- W2063485138 title "Phospholipase D in homogenates from HL-60 granulocytes: Implications of calcium and G protein control" @default.
- W2063485138 cites W1493283928 @default.
- W2063485138 cites W1513050536 @default.
- W2063485138 cites W1528198451 @default.
- W2063485138 cites W1574428487 @default.
- W2063485138 cites W1605324556 @default.
- W2063485138 cites W1966270555 @default.
- W2063485138 cites W1972703706 @default.
- W2063485138 cites W2001588855 @default.
- W2063485138 cites W2015546115 @default.
- W2063485138 cites W2025092789 @default.
- W2063485138 cites W2036194106 @default.
- W2063485138 cites W2045194506 @default.
- W2063485138 cites W2059897580 @default.
- W2063485138 cites W2063352190 @default.
- W2063485138 cites W2064035247 @default.
- W2063485138 cites W207203228 @default.
- W2063485138 cites W2133404596 @default.
- W2063485138 cites W2152311269 @default.
- W2063485138 cites W4293247451 @default.
- W2063485138 doi "https://doi.org/10.1016/0006-291x(89)92187-6" @default.
- W2063485138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2505772" @default.
- W2063485138 hasPublicationYear "1989" @default.
- W2063485138 type Work @default.
- W2063485138 sameAs 2063485138 @default.
- W2063485138 citedByCount "88" @default.
- W2063485138 countsByYear W20634851382012 @default.
- W2063485138 countsByYear W20634851382014 @default.
- W2063485138 crossrefType "journal-article" @default.
- W2063485138 hasAuthorship W2063485138A5005757827 @default.
- W2063485138 hasAuthorship W2063485138A5024512000 @default.
- W2063485138 hasAuthorship W2063485138A5046002593 @default.
- W2063485138 hasAuthorship W2063485138A5046195597 @default.
- W2063485138 hasAuthorship W2063485138A5049562626 @default.
- W2063485138 hasConcept C12927208 @default.
- W2063485138 hasConcept C178790620 @default.
- W2063485138 hasConcept C181199279 @default.
- W2063485138 hasConcept C185592680 @default.
- W2063485138 hasConcept C2776330855 @default.
- W2063485138 hasConcept C2776909261 @default.
- W2063485138 hasConcept C2778918659 @default.
- W2063485138 hasConcept C2780202252 @default.
- W2063485138 hasConcept C2780263894 @default.
- W2063485138 hasConcept C2780610907 @default.
- W2063485138 hasConcept C2781170229 @default.
- W2063485138 hasConcept C2909482567 @default.
- W2063485138 hasConcept C41625074 @default.
- W2063485138 hasConcept C55493867 @default.
- W2063485138 hasConcept C6182249 @default.
- W2063485138 hasConcept C62478195 @default.
- W2063485138 hasConcept C71240020 @default.
- W2063485138 hasConceptScore W2063485138C12927208 @default.
- W2063485138 hasConceptScore W2063485138C178790620 @default.
- W2063485138 hasConceptScore W2063485138C181199279 @default.
- W2063485138 hasConceptScore W2063485138C185592680 @default.
- W2063485138 hasConceptScore W2063485138C2776330855 @default.
- W2063485138 hasConceptScore W2063485138C2776909261 @default.
- W2063485138 hasConceptScore W2063485138C2778918659 @default.
- W2063485138 hasConceptScore W2063485138C2780202252 @default.
- W2063485138 hasConceptScore W2063485138C2780263894 @default.
- W2063485138 hasConceptScore W2063485138C2780610907 @default.
- W2063485138 hasConceptScore W2063485138C2781170229 @default.
- W2063485138 hasConceptScore W2063485138C2909482567 @default.
- W2063485138 hasConceptScore W2063485138C41625074 @default.
- W2063485138 hasConceptScore W2063485138C55493867 @default.
- W2063485138 hasConceptScore W2063485138C6182249 @default.
- W2063485138 hasConceptScore W2063485138C62478195 @default.
- W2063485138 hasConceptScore W2063485138C71240020 @default.
- W2063485138 hasIssue "1" @default.
- W2063485138 hasLocation W20634851381 @default.
- W2063485138 hasLocation W20634851382 @default.
- W2063485138 hasOpenAccess W2063485138 @default.
- W2063485138 hasPrimaryLocation W20634851381 @default.
- W2063485138 hasRelatedWork W1018543304 @default.
- W2063485138 hasRelatedWork W1619926068 @default.
- W2063485138 hasRelatedWork W1967000577 @default.
- W2063485138 hasRelatedWork W1974995836 @default.
- W2063485138 hasRelatedWork W1984650324 @default.
- W2063485138 hasRelatedWork W2016980336 @default.
- W2063485138 hasRelatedWork W2020241626 @default.
- W2063485138 hasRelatedWork W2063485138 @default.
- W2063485138 hasRelatedWork W2156692562 @default.
- W2063485138 hasRelatedWork W2416579342 @default.
- W2063485138 hasVolume "163" @default.
- W2063485138 isParatext "false" @default.
- W2063485138 isRetracted "false" @default.
- W2063485138 magId "2063485138" @default.
- W2063485138 workType "article" @default.