Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063513003> ?p ?o ?g. }
- W2063513003 endingPage "438" @default.
- W2063513003 startingPage "429" @default.
- W2063513003 abstract "Resonance Raman (RR) and surface-enhanced resonance Raman (SERR) spectroscopy were used to study the behavior of 2-(4′-hydroxyphenylazo)benzoic acid (HABA) at different pH values (2.7–11.8) in solution and bound to avidin. Major changes in the RR spectrum of the dye are observed near pH 8.0 and 3.0. These pH values are close to the pKa values for the phenolic and carboxylic acid functional groups of HABA and reflect deprotonation of these groups. In addition, however, tautomeric forms of the dye are possible within each pH range and these can be identified from the characteristic vibrational modes of the azo and hydrazone forms of HABA. Similar changes in the SERR spectrum of HABA were also observed as a function of pH. However, a comparison of the RR and SERR spectra has shown that the tautomeric equilbrium is shifted toward the hydrazone form on the Ag surface compared with the effect in solution. The RR and SERR spectra of the HABA-avidin complex are nearly identical and indicative of the hydrazone form of the dye. Hence the complex is not dissociated on the Ag surface. This finding is encouraging with regard to the application of SERR techniques to the study of native proteins containing chromophores." @default.
- W2063513003 created "2016-06-24" @default.
- W2063513003 creator A5012583152 @default.
- W2063513003 creator A5042664623 @default.
- W2063513003 date "1988-09-01" @default.
- W2063513003 modified "2023-10-16" @default.
- W2063513003 title "Surface-enhanced resonance Raman study of avidin-dye interactions: A model for chromophore-containing proteins" @default.
- W2063513003 cites W1736394754 @default.
- W2063513003 cites W1975682090 @default.
- W2063513003 cites W1979195745 @default.
- W2063513003 cites W1980400933 @default.
- W2063513003 cites W1981356526 @default.
- W2063513003 cites W1984367971 @default.
- W2063513003 cites W1985818365 @default.
- W2063513003 cites W1987282286 @default.
- W2063513003 cites W1995775220 @default.
- W2063513003 cites W1997311439 @default.
- W2063513003 cites W2006958782 @default.
- W2063513003 cites W2007868426 @default.
- W2063513003 cites W2014909342 @default.
- W2063513003 cites W2016859383 @default.
- W2063513003 cites W2024236859 @default.
- W2063513003 cites W2025234430 @default.
- W2063513003 cites W2030618864 @default.
- W2063513003 cites W2032570649 @default.
- W2063513003 cites W2035014821 @default.
- W2063513003 cites W2049480828 @default.
- W2063513003 cites W2056744989 @default.
- W2063513003 cites W2061123500 @default.
- W2063513003 cites W2070220636 @default.
- W2063513003 cites W2081073868 @default.
- W2063513003 cites W2082302149 @default.
- W2063513003 cites W2086971829 @default.
- W2063513003 cites W2088923284 @default.
- W2063513003 cites W2089429529 @default.
- W2063513003 cites W2091677485 @default.
- W2063513003 cites W2127702122 @default.
- W2063513003 cites W2155823706 @default.
- W2063513003 cites W2282256421 @default.
- W2063513003 cites W2293438303 @default.
- W2063513003 cites W2316959424 @default.
- W2063513003 doi "https://doi.org/10.1002/jrs.1250190610" @default.
- W2063513003 hasPublicationYear "1988" @default.
- W2063513003 type Work @default.
- W2063513003 sameAs 2063513003 @default.
- W2063513003 citedByCount "29" @default.
- W2063513003 crossrefType "journal-article" @default.
- W2063513003 hasAuthorship W2063513003A5012583152 @default.
- W2063513003 hasAuthorship W2063513003A5042664623 @default.
- W2063513003 hasConcept C109214941 @default.
- W2063513003 hasConcept C111233374 @default.
- W2063513003 hasConcept C113196181 @default.
- W2063513003 hasConcept C118629725 @default.
- W2063513003 hasConcept C120665830 @default.
- W2063513003 hasConcept C121332964 @default.
- W2063513003 hasConcept C139210041 @default.
- W2063513003 hasConcept C145148216 @default.
- W2063513003 hasConcept C178790620 @default.
- W2063513003 hasConcept C185592680 @default.
- W2063513003 hasConcept C192468462 @default.
- W2063513003 hasConcept C2778844314 @default.
- W2063513003 hasConcept C2780349576 @default.
- W2063513003 hasConcept C40003534 @default.
- W2063513003 hasConcept C71240020 @default.
- W2063513003 hasConcept C75473681 @default.
- W2063513003 hasConceptScore W2063513003C109214941 @default.
- W2063513003 hasConceptScore W2063513003C111233374 @default.
- W2063513003 hasConceptScore W2063513003C113196181 @default.
- W2063513003 hasConceptScore W2063513003C118629725 @default.
- W2063513003 hasConceptScore W2063513003C120665830 @default.
- W2063513003 hasConceptScore W2063513003C121332964 @default.
- W2063513003 hasConceptScore W2063513003C139210041 @default.
- W2063513003 hasConceptScore W2063513003C145148216 @default.
- W2063513003 hasConceptScore W2063513003C178790620 @default.
- W2063513003 hasConceptScore W2063513003C185592680 @default.
- W2063513003 hasConceptScore W2063513003C192468462 @default.
- W2063513003 hasConceptScore W2063513003C2778844314 @default.
- W2063513003 hasConceptScore W2063513003C2780349576 @default.
- W2063513003 hasConceptScore W2063513003C40003534 @default.
- W2063513003 hasConceptScore W2063513003C71240020 @default.
- W2063513003 hasConceptScore W2063513003C75473681 @default.
- W2063513003 hasIssue "6" @default.
- W2063513003 hasLocation W20635130031 @default.
- W2063513003 hasOpenAccess W2063513003 @default.
- W2063513003 hasPrimaryLocation W20635130031 @default.
- W2063513003 hasRelatedWork W1977997166 @default.
- W2063513003 hasRelatedWork W1986627169 @default.
- W2063513003 hasRelatedWork W2009234021 @default.
- W2063513003 hasRelatedWork W2024077355 @default.
- W2063513003 hasRelatedWork W2050021938 @default.
- W2063513003 hasRelatedWork W2105466274 @default.
- W2063513003 hasRelatedWork W2181811547 @default.
- W2063513003 hasRelatedWork W2367778059 @default.
- W2063513003 hasRelatedWork W2374506354 @default.
- W2063513003 hasRelatedWork W4245388779 @default.
- W2063513003 hasVolume "19" @default.
- W2063513003 isParatext "false" @default.
- W2063513003 isRetracted "false" @default.