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• W2063513729 abstract "Abstract The glucuronoxylomannan (GXM) of C. neoformans serotype A contained molar ratios of xylose: mannose: glucuronic acid: O -acetyl of 2:5:1:2. The xylose and glucuronic acid occurred as single pyranosyl residues and were oxidized by periodate; the Smith-degraded product was an insoluble mannan. Mannose was linked (1→3) with three out of five mannose residues (1→2)-substituted with one glucuronosyl and two single xylosyl end groups. A repeating octasaccharide was proposed as the structure. GXM was separated into species-specific and serotype-specific components by chromatography on a serotype-D-antibody agarose column, but these showed no obvious differences in molar ratios of the monosaccharides. Decreased serological activity was observed when GXM was de- O -acetylated or carboxy-reduced, but not when carboxy-esterified. The Smith-degraded mannan was inactive. Autohydrolysis caused a gradual release of xylose and some mannose, and a decline in serological activity. Xylose and glucuronic acid were implicated in a single antigenic group since selective inactivation of either residue led to loss of more than half of the activity. Extensive crossreactions were observed with the GXMs from C. laurentii and Tremella mesenterica , but not with types II or XIV pneumococcal polysaccharides." @default.
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• W2063513729 date "1980-08-01" @default.
• W2063513729 modified "2023-09-25" @default.
• W2063513729 title "Structure and antigenic activity of the capsular polysaccharide of Cryptococcus neoformans serotype A" @default.
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• W2063513729 doi "https://doi.org/10.1016/0161-5890(80)90096-6" @default.
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