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- W2063527992 abstract "We describe the use of scanning confocal fluorescence microscopy to probe the structure and temporal dynamics of unsupported planar lipid bilayer membranes. At high fluorescent label concentration, the shape and stability of the membranes are evaluated with submicron spatial resolution. At low label concentration, we observe individual molecules moving into and out of the confocal laser beam in real time. Lipid diffusion coefficients are measured via fluctuation correlation spectroscopy (FCS) and indicate nominal values of (0.1 +/- 0.2) X 10-7 cm2/sec homogeneously distributed over the central portion of the bilayer. We also compare the real-time single-molecule fluorescence signatures to simulated photon emission patterns generated by a 2D random-walk model. Interestingly, analysis of the interval between fluorescence events suggests the presence of a diffusion bias that can be explained by a weak optical trapping mechanism. The trap causes labeled lipids to return to the confocal detection region more frequently than pure Einsteinian diffusion predicts.© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only." @default.
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- W2063527992 date "2000-04-21" @default.
- W2063527992 modified "2023-09-23" @default.
- W2063527992 title "<title>Interrogating unsupported lipid bilayers with scanning confocal single-molecule fluorescence microscopy</title>" @default.
- W2063527992 doi "https://doi.org/10.1117/12.383357" @default.
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