Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063551005> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2063551005 endingPage "1194" @default.
- W2063551005 startingPage "1191" @default.
- W2063551005 abstract "Abstract The emergence of antisense and antigene oligonucleotides as potential sequenceselective inhibitors of gene expression is evidenced by the growing number of ongoing clinicals trials against a variety of diseases. First generation antisense therapeutics utilize a uniformly modified oligodeoxyribonucleotide phosphorothioate where one non-bridging oxygen atom is formally replaced by sulfur, because natural DNA is unstable towards extra- and intracellular enzymes. Phosphoramidite chemistry has been widely used for the synthesis of phosphorothioate oligonucleotides because of its potential for automation, high coupling efficiency, ease of site-specific thioate linkage incorporation, and ready scalability. The large scale solid-supported synthesis of phosphorothioates is presently carried out by initial formation of the internucleotidic phosphite linkage followed by sulfurization of the phosphite triester to phosphorothioate using the Beaucage reagent. The resulting O,O-linked phosphorothioate diester linkage in the oligonucleotide is a chiral functional group. For a typical 20-mer there are 524,288 (219) possible diastereoisomers. Separation and individual quantification of this number of diastereomers is currently not feasible. In addition, the best reported methods for stereocontrolled synthesis of phosphorothioate oligomers are not presently useful for drug synthesis; that is, since net 100% enantiomeric excess is not achieved in the coupling step, the oligomeric product still consists of the same mixture of Sp and Rp diastereomers, except that the levels of all but one isomer are reduced to low individual levels. As a result, even a small change in the and Sp phosphorothioate diesters, due to racemization during coupling, indicating that the overall synthetic process is stereo reproducible and under inherent process control." @default.
- W2063551005 created "2016-06-24" @default.
- W2063551005 creator A5008292150 @default.
- W2063551005 creator A5032349179 @default.
- W2063551005 creator A5040424450 @default.
- W2063551005 creator A5061174068 @default.
- W2063551005 date "1999-06-01" @default.
- W2063551005 modified "2023-09-26" @default.
- W2063551005 title "Diastereomeric Process Control in the Synthesis of Oligodeoxyribonucleotide Phosphorothioates" @default.
- W2063551005 cites W1987044618 @default.
- W2063551005 cites W2003832440 @default.
- W2063551005 cites W2012942984 @default.
- W2063551005 cites W2062575651 @default.
- W2063551005 cites W2072696166 @default.
- W2063551005 cites W2077002341 @default.
- W2063551005 cites W2179618970 @default.
- W2063551005 doi "https://doi.org/10.1080/07328319908044659" @default.
- W2063551005 hasPublicationYear "1999" @default.
- W2063551005 type Work @default.
- W2063551005 sameAs 2063551005 @default.
- W2063551005 citedByCount "2" @default.
- W2063551005 countsByYear W20635510052021 @default.
- W2063551005 crossrefType "journal-article" @default.
- W2063551005 hasAuthorship W2063551005A5008292150 @default.
- W2063551005 hasAuthorship W2063551005A5032349179 @default.
- W2063551005 hasAuthorship W2063551005A5040424450 @default.
- W2063551005 hasAuthorship W2063551005A5061174068 @default.
- W2063551005 hasConcept C129312508 @default.
- W2063551005 hasConcept C138716334 @default.
- W2063551005 hasConcept C178790620 @default.
- W2063551005 hasConcept C185592680 @default.
- W2063551005 hasConcept C21951064 @default.
- W2063551005 hasConcept C2776043002 @default.
- W2063551005 hasConcept C40875361 @default.
- W2063551005 hasConcept C486523 @default.
- W2063551005 hasConcept C552990157 @default.
- W2063551005 hasConcept C55493867 @default.
- W2063551005 hasConcept C60002323 @default.
- W2063551005 hasConcept C71240020 @default.
- W2063551005 hasConceptScore W2063551005C129312508 @default.
- W2063551005 hasConceptScore W2063551005C138716334 @default.
- W2063551005 hasConceptScore W2063551005C178790620 @default.
- W2063551005 hasConceptScore W2063551005C185592680 @default.
- W2063551005 hasConceptScore W2063551005C21951064 @default.
- W2063551005 hasConceptScore W2063551005C2776043002 @default.
- W2063551005 hasConceptScore W2063551005C40875361 @default.
- W2063551005 hasConceptScore W2063551005C486523 @default.
- W2063551005 hasConceptScore W2063551005C552990157 @default.
- W2063551005 hasConceptScore W2063551005C55493867 @default.
- W2063551005 hasConceptScore W2063551005C60002323 @default.
- W2063551005 hasConceptScore W2063551005C71240020 @default.
- W2063551005 hasIssue "6-7" @default.
- W2063551005 hasLocation W20635510051 @default.
- W2063551005 hasOpenAccess W2063551005 @default.
- W2063551005 hasPrimaryLocation W20635510051 @default.
- W2063551005 hasRelatedWork W1965874718 @default.
- W2063551005 hasRelatedWork W1985446798 @default.
- W2063551005 hasRelatedWork W2045238150 @default.
- W2063551005 hasRelatedWork W2073998753 @default.
- W2063551005 hasRelatedWork W2142229498 @default.
- W2063551005 hasRelatedWork W2172848752 @default.
- W2063551005 hasRelatedWork W2952430552 @default.
- W2063551005 hasRelatedWork W2952462956 @default.
- W2063551005 hasRelatedWork W2956376780 @default.
- W2063551005 hasRelatedWork W2111604832 @default.
- W2063551005 hasVolume "18" @default.
- W2063551005 isParatext "false" @default.
- W2063551005 isRetracted "false" @default.
- W2063551005 magId "2063551005" @default.
- W2063551005 workType "article" @default.