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- W2063562100 abstract "Abstract Treatment of TYMV (turnip yellow mosaic virus) virions under alkaline conditions and at high ionic strength leads to the in situ formation of RNA sedimenting uniformly at 38 S. Native TYMV-RNA has a sedimentation coefficient of 28 S in the same solvent. A systematic study in which the temperature, ionic strength, and pH were varied showed that favorable conditions for 38 S RNA formation are 1.0 M KCl, pH 10.5, 30°, and a reaction time of 5–10 min. Nonetheless, the fast-sedimenting RNA can also be obtained at neutral pH and 1.0 M KCl, provided that the time of treatment is prolonged or the temperature is raised. No evidence for breakage of the RNA chain was obtained under the latter conditions, while all of the RNA is retained by the virion. These data indicate that exposure of TYMV to high ionic strength causes a drastic structural rearrangement of the viral RNA inside the capsid without concurrent fragmentation of the RNA chain." @default.
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- W2063562100 date "1977-02-01" @default.
- W2063562100 modified "2023-09-25" @default.
- W2063562100 title "The formation of fast-sedimenting turnip yellow mosaic virus RNA: structural rearrangement inside the capsid" @default.
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- W2063562100 doi "https://doi.org/10.1016/0042-6822(77)90258-6" @default.
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