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- W2063636613 abstract "The binding of a protein to an RNA sequence protects the region of the RNA from cleavage by chemicals or RNases; this protected region is known as the protein's footprint. In the footprinting protocol presented here, end-labeled RNAs with and without bound protein are cleaved using chemical methods. Fe(II)-EDTA is used to generate hydroxyl radicals in the presence of a reducing agent. These hydroxyl radicals indiscriminately cleave ribose groups in regions of the ribose-phosphate backbone that are exposed to solvent. After termination of cleavage, the resulting RNA fragments are analyzed by gel electrophoresis on denaturing polyacrylamide gels. Because hydroxyl radicals are smaller and cleave less specifically than RNases, this approach, if feasible, is often the method of choice for monitoring sites of RNA-protein interactions." @default.
- W2063636613 created "2016-06-24" @default.
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- W2063636613 date "2014-12-01" @default.
- W2063636613 modified "2023-10-14" @default.
- W2063636613 title "Mapping RNA–Protein Interactions Using Hydroxyl-Radical Footprinting" @default.
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- W2063636613 doi "https://doi.org/10.1101/pdb.prot080952" @default.
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