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• W2063645401 abstract "As a non-viral gene vector, long PEI chains are more effective but also more cytotoxic. To solve this problem, people have tried to use disulfide (-S-S-) to link short PEI chains into a long one to generate highly efficient and less cytotoxic gene vectors because -S-S- is degradable inside the cell. In order to investigate when and where -S-S- is cleaved during intracellular trafficking, we designed and synthesized rhodamine B labeled linear PEI chains (Mn≈ 3 kg mol-1) with one end modified with a mercapto-group so that they can be coupled together via one disulfide bond in the middle and one rhodamine B molecule on each side of the disulfide bond, where fluorescence is self-quenched because two rhodamine B molecules are closely linked together. The cleavage of the -S-S- bond separates the two rhodamine B molecules and enhances their fluorescent intensity. In addition, plasmid DNA was also modified with bodipy, a FRET donor of rhodamine B. Using this specially prepared PEI, we studied the intracellular trafficking of the PEI/DNA polyplexes by using flow cytometry and confocal laser scanning microscopy. Our results reveal that (1) DNA is gradually dissociated from the polyplex before the disulfide bond's cleavage; (2) some of polyplexes escaped from endosomes before reaching lysosomes; and (3) the disulfide bonds are mainly cleaved inside lysosomes at ∼5 h post-transfection." @default.
• W2063645401 created "2016-06-24" @default.
• W2063645401 creator A5058870726 @default.
• W2063645401 creator A5088444614 @default.
• W2063645401 date "2014-01-01" @default.
• W2063645401 modified "2023-10-18" @default.
• W2063645401 title "Revisiting the complexation between DNA and polyethylenimine – when and where –S–S– linked PEI is cleaved inside the cell" @default.
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• W2063645401 doi "https://doi.org/10.1039/c4tb00031e" @default.
• W2063645401 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32261590" @default.
• W2063645401 hasPublicationYear "2014" @default.
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