FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063660318> ?p ?o ?g. }

• W2063660318 endingPage "137" @default.
• W2063660318 startingPage "137" @default.
• W2063660318 abstract "Although prior heat stress (HS) inhibits apoptosis in adenosine phosphate (ATP)-depleted renal epithelial cells (REC), the specific stress protein(s) responsible for cytoprotection have not been identified. The present study evaluated the hypothesis that Hsp72, the major inducible member of the Hsp70 family, protects REC against ATP depletion injury. In the presence of isopropyl-beta-D-thiogalactoside (IPTG), a stable line of transfected opossum kidney cells was induced to overexpress human Hsp72 tagged with the flag epitope. Transfected cells from 2 clones that expressed Hsp72 at a level comparable with wild-type cells were subjected to transient heat stress (43 degrees C for 1 hour). To assess the cytoprotective effect of Hsp72, transfected cells were subjected to transient ATP depletion followed by recovery in the presence vs the absence of IPTG. ATP depletion resulted in nuclear chromatin condensation without cell membrane injury (ie, minimal leak of lactate dehydrogenase) and activation of caspase-3, confirming that apoptosis is the major cause of cell death. In both clones cell survival 1-3 days after ATP depletion was significantly improved in the presence of IPTG. Selective overexpression of Hsp72 reproduced nearly 60% of the protective effect on the survival afforded by prior heat stress. In transfected cells subjected to ATP depletion, Hsp72 overexpression significantly inhibited caspase activation. In native renal cells brief ATP depletion markedly induced the expression of native Hsp72, a finding identical to that observed after renal ischemia in vivo. These studies are the first to directly show that Hsp72 per se mediates acquired resistance to ischemic injury in REC." @default.
• W2063660318 created "2016-06-24" @default.
• W2063660318 creator A5012983379 @default.
• W2063660318 creator A5033295786 @default.
• W2063660318 creator A5085730657 @default.
• W2063660318 creator A5091215873 @default.
• W2063660318 date "2002-01-01" @default.
• W2063660318 modified "2023-10-13" @default.
• W2063660318 title "Hsp72 expression enhances survival in adenosine triphosphate–depleted renal epithelial cells" @default.
• W2063660318 cites W153442697 @default.
• W2063660318 cites W1561662137 @default.
• W2063660318 cites W162515504 @default.
• W2063660318 cites W1769448430 @default.
• W2063660318 cites W1954929088 @default.
• W2063660318 cites W1964478301 @default.
• W2063660318 cites W1965242966 @default.
• W2063660318 cites W1966502113 @default.
• W2063660318 cites W1979450279 @default.
• W2063660318 cites W1983765506 @default.
• W2063660318 cites W1985731963 @default.
• W2063660318 cites W1993746101 @default.
• W2063660318 cites W1995865008 @default.
• W2063660318 cites W2007116202 @default.
• W2063660318 cites W2008343355 @default.
• W2063660318 cites W2009372017 @default.
• W2063660318 cites W2018886962 @default.
• W2063660318 cites W2035796688 @default.
• W2063660318 cites W2059901120 @default.
• W2063660318 cites W2061738083 @default.
• W2063660318 cites W2072001004 @default.
• W2063660318 cites W2079321385 @default.
• W2063660318 cites W2084736894 @default.
• W2063660318 cites W2089776907 @default.
• W2063660318 cites W2093525170 @default.
• W2063660318 cites W2107789276 @default.
• W2063660318 cites W2109054322 @default.
• W2063660318 cites W2111858669 @default.
• W2063660318 cites W2119683782 @default.
• W2063660318 cites W2132889264 @default.
• W2063660318 cites W2137952321 @default.
• W2063660318 cites W2156818312 @default.
• W2063660318 cites W2161602870 @default.
• W2063660318 cites W2164108483 @default.
• W2063660318 cites W2164340078 @default.
• W2063660318 cites W2189371217 @default.
• W2063660318 cites W2200076371 @default.
• W2063660318 cites W2214396615 @default.
• W2063660318 cites W2233325915 @default.
• W2063660318 cites W2235325702 @default.
• W2063660318 cites W2312173669 @default.
• W2063660318 cites W2339572250 @default.
• W2063660318 cites W2401199013 @default.
• W2063660318 cites W2418500986 @default.
• W2063660318 cites W2418745439 @default.
• W2063660318 cites W2439478110 @default.
• W2063660318 doi "https://doi.org/10.1379/1466-1268(2002)007<0137:heesia>2.0.co;2" @default.
• W2063660318 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/514811" @default.
• W2063660318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12380681" @default.
• W2063660318 hasPublicationYear "2002" @default.
• W2063660318 type Work @default.
• W2063660318 sameAs 2063660318 @default.
• W2063660318 citedByCount "25" @default.
• W2063660318 countsByYear W20636603182012 @default.
• W2063660318 countsByYear W20636603182013 @default.
• W2063660318 countsByYear W20636603182014 @default.
• W2063660318 countsByYear W20636603182019 @default.
• W2063660318 crossrefType "journal-article" @default.
• W2063660318 hasAuthorship W2063660318A5012983379 @default.
• W2063660318 hasAuthorship W2063660318A5033295786 @default.
• W2063660318 hasAuthorship W2063660318A5085730657 @default.
• W2063660318 hasAuthorship W2063660318A5091215873 @default.
• W2063660318 hasBestOaLocation W20636603182 @default.
• W2063660318 hasConcept C104317684 @default.
• W2063660318 hasConcept C153911025 @default.
• W2063660318 hasConcept C190283241 @default.
• W2063660318 hasConcept C205260736 @default.
• W2063660318 hasConcept C2780967304 @default.
• W2063660318 hasConcept C31573885 @default.
• W2063660318 hasConcept C54009773 @default.
• W2063660318 hasConcept C54355233 @default.
• W2063660318 hasConcept C55493867 @default.
• W2063660318 hasConcept C81885089 @default.
• W2063660318 hasConcept C86803240 @default.
• W2063660318 hasConcept C95444343 @default.
• W2063660318 hasConceptScore W2063660318C104317684 @default.
• W2063660318 hasConceptScore W2063660318C153911025 @default.
• W2063660318 hasConceptScore W2063660318C190283241 @default.
• W2063660318 hasConceptScore W2063660318C205260736 @default.
• W2063660318 hasConceptScore W2063660318C2780967304 @default.
• W2063660318 hasConceptScore W2063660318C31573885 @default.
• W2063660318 hasConceptScore W2063660318C54009773 @default.
• W2063660318 hasConceptScore W2063660318C54355233 @default.
• W2063660318 hasConceptScore W2063660318C55493867 @default.
• W2063660318 hasConceptScore W2063660318C81885089 @default.
• W2063660318 hasConceptScore W2063660318C86803240 @default.
• W2063660318 hasConceptScore W2063660318C95444343 @default.
• W2063660318 hasIssue "2" @default.
• W2063660318 hasLocation W20636603181 @default.

• next