Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063670999> ?p ?o ?g. }
- W2063670999 endingPage "3954" @default.
- W2063670999 startingPage "3948" @default.
- W2063670999 abstract "Phosphoglycerate mutase (PGM; EC 2.7.5.3) isolated from rat and rabbit muscle has been shown to possess an unusually long-lived fluorescence component when excited by ultraviolet light below 310 nm. On the basis of spectral and physical measurements, this 16.4 (+/- 0.2) ns fluorescence lifetime at room temperature is assigned to a tryptophan residue in an unusual environment. The emission profile of this long-lived tryptophan is red shifted from the other tryptophans of PGM by approximately 25 nm. PGM has been crystallized and sequenced from yeast where it has been shown to be a tetramer with 29K subunits. However, we have not been able to detect the existence of an unusually long-lived fluorescence component in the yeast isomer. The long fluorescence lifetime is lost upon denaturation of rabbit PGM and is partially restored upon introduction of the protein to a nondenaturing environment, suggesting the long lifetime is not the result of a covalent modification. The PGM molecule was studied by a number of techniques including time-resolved tryptophan fluorescence, quenching studies of tryptophan fluorescence, and enzyme activity studies. The long-lived fluorescence has been shown to be statistically quenched by Br-, I-, and Cu2+ in the submillimolar region while the acrylamide quenching shows the tryptophan is marginally accessible to solvent. Characterization of the long-lived fluorescence and its possible sources are discussed." @default.
- W2063670999 created "2016-06-24" @default.
- W2063670999 creator A5074412117 @default.
- W2063670999 creator A5086291810 @default.
- W2063670999 date "1989-05-02" @default.
- W2063670999 modified "2023-09-27" @default.
- W2063670999 title "Long-lived tryptophan fluorescence in phosphoglycerate mutase" @default.
- W2063670999 cites W1503638876 @default.
- W2063670999 cites W1540008704 @default.
- W2063670999 cites W1540879490 @default.
- W2063670999 cites W1561202599 @default.
- W2063670999 cites W1816966864 @default.
- W2063670999 cites W1894953813 @default.
- W2063670999 cites W1964766451 @default.
- W2063670999 cites W1974399076 @default.
- W2063670999 cites W1980129106 @default.
- W2063670999 cites W1986687869 @default.
- W2063670999 cites W1987112003 @default.
- W2063670999 cites W1992403473 @default.
- W2063670999 cites W1993442337 @default.
- W2063670999 cites W1999312513 @default.
- W2063670999 cites W2006301525 @default.
- W2063670999 cites W2009757641 @default.
- W2063670999 cites W2014138285 @default.
- W2063670999 cites W2014265237 @default.
- W2063670999 cites W2016129286 @default.
- W2063670999 cites W2025461938 @default.
- W2063670999 cites W2035221292 @default.
- W2063670999 cites W2035663766 @default.
- W2063670999 cites W2036531156 @default.
- W2063670999 cites W2037016705 @default.
- W2063670999 cites W2037064359 @default.
- W2063670999 cites W2039665613 @default.
- W2063670999 cites W2041487385 @default.
- W2063670999 cites W2045524264 @default.
- W2063670999 cites W2047033055 @default.
- W2063670999 cites W2058659093 @default.
- W2063670999 cites W2061667560 @default.
- W2063670999 cites W2064243534 @default.
- W2063670999 cites W2065135879 @default.
- W2063670999 cites W2076032984 @default.
- W2063670999 cites W2077510801 @default.
- W2063670999 cites W2077516971 @default.
- W2063670999 cites W2080796053 @default.
- W2063670999 cites W2087070363 @default.
- W2063670999 cites W2089023077 @default.
- W2063670999 cites W2094186244 @default.
- W2063670999 cites W2097780360 @default.
- W2063670999 cites W2102529685 @default.
- W2063670999 cites W2413316384 @default.
- W2063670999 cites W75724847 @default.
- W2063670999 cites W958087039 @default.
- W2063670999 doi "https://doi.org/10.1021/bi00435a048" @default.
- W2063670999 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2546587" @default.
- W2063670999 hasPublicationYear "1989" @default.
- W2063670999 type Work @default.
- W2063670999 sameAs 2063670999 @default.
- W2063670999 citedByCount "17" @default.
- W2063670999 countsByYear W20636709992012 @default.
- W2063670999 countsByYear W20636709992014 @default.
- W2063670999 countsByYear W20636709992017 @default.
- W2063670999 countsByYear W20636709992018 @default.
- W2063670999 crossrefType "journal-article" @default.
- W2063670999 hasAuthorship W2063670999A5074412117 @default.
- W2063670999 hasAuthorship W2063670999A5086291810 @default.
- W2063670999 hasConcept C121332964 @default.
- W2063670999 hasConcept C121745418 @default.
- W2063670999 hasConcept C181199279 @default.
- W2063670999 hasConcept C185592680 @default.
- W2063670999 hasConcept C20251656 @default.
- W2063670999 hasConcept C2776706248 @default.
- W2063670999 hasConcept C2778886173 @default.
- W2063670999 hasConcept C515207424 @default.
- W2063670999 hasConcept C55493867 @default.
- W2063670999 hasConcept C62520636 @default.
- W2063670999 hasConcept C75473681 @default.
- W2063670999 hasConcept C91754966 @default.
- W2063670999 hasConcept C91881484 @default.
- W2063670999 hasConceptScore W2063670999C121332964 @default.
- W2063670999 hasConceptScore W2063670999C121745418 @default.
- W2063670999 hasConceptScore W2063670999C181199279 @default.
- W2063670999 hasConceptScore W2063670999C185592680 @default.
- W2063670999 hasConceptScore W2063670999C20251656 @default.
- W2063670999 hasConceptScore W2063670999C2776706248 @default.
- W2063670999 hasConceptScore W2063670999C2778886173 @default.
- W2063670999 hasConceptScore W2063670999C515207424 @default.
- W2063670999 hasConceptScore W2063670999C55493867 @default.
- W2063670999 hasConceptScore W2063670999C62520636 @default.
- W2063670999 hasConceptScore W2063670999C75473681 @default.
- W2063670999 hasConceptScore W2063670999C91754966 @default.
- W2063670999 hasConceptScore W2063670999C91881484 @default.
- W2063670999 hasIssue "9" @default.
- W2063670999 hasLocation W20636709991 @default.
- W2063670999 hasLocation W20636709992 @default.
- W2063670999 hasOpenAccess W2063670999 @default.
- W2063670999 hasPrimaryLocation W20636709991 @default.
- W2063670999 hasRelatedWork W1994239116 @default.
- W2063670999 hasRelatedWork W2024752246 @default.