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- W2063672367 abstract "This introductory chapter reviews the history of microbeams starting with the original UV microbeam work of Tchakhotine in 1912 and covers the progress and application of microbeams through 2006. The main focus of the chapter is on laser “scissors” starting with Marcel Bessis' and colleagues work with the ruby laser microbeam in Paris in 1962. Following this introduction, a section is devoted to describing the different laser microbeam systems and then the rest of the chapter is devoted to applications in cell and developmental biology. The approach is to focus on the organelle/structure and describe how the laser microbeam has been applied to studying its structure and/or function. Since considerable work has been done on chromosomes and the mitotic spindle (Section V.A and C), these topics have been divided in distinct subsections. Other topics discussed are injection of foreign DNA through the cell membrane (optoporation/optoinjection), cell migration, the nucleolus, mitochondria, cytoplasmic filaments, and embryos fate‐mapping. A final technology section is devoted to discussing the pros and cons of building/buying your own laser microbeam system and the option of using the Internet‐based RoboLase system. Throughout the chapter, reference is made to other chapters in the book that go into more detail on the subjects briefly mentioned." @default.
- W2063672367 created "2016-06-24" @default.
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- W2063672367 date "1971-04-01" @default.
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- W2063672367 title "A simple and versatile argon laser microbeam" @default.
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- W2063672367 doi "https://doi.org/10.1016/0014-4827(71)90030-9" @default.
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