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- W2063672735 abstract "The flagella of Chlamydomonas can be detached from the cell bodies by calcium ions. These flagella round up and some of the axoneme protein goes into solution when they are resuspended in a buffer containing magnesium as used by Gibbons (1963) and Watson & Hynes (1966) for preserving Tetrahymena cilia. Flagella showing a normal transverse section and good membrane preservation may be obtained by including calcium in the magnesium buffer. The axoneme proteins of Chlamydomonas flagella have different solubility properties to the previously studied Tetrahymena cilia (Watson et al . 1966; Renaud, Rowe & Gibbons 1968). A low ionic strength buffer containing EDTA extracts the protein of one of the central pair fibres and part of one of each of the subfibres of the outer nine. The outer nine fibres are not dissolved by dialysis against 0∙6M potassium chloride but are soluble in ionic detergents." @default.
- W2063672735 created "2016-06-24" @default.
- W2063672735 date "1969-04-15" @default.
- W2063672735 modified "2023-09-26" @default.
- W2063672735 title "Biochemistry of Chlamydomonas flagella" @default.
- W2063672735 doi "https://doi.org/10.1098/rspb.1969.0037" @default.
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