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• W2063697785 abstract "Mesenchymal stem cells (MSC) show a very short proliferative life span and readily lose the differentiation potential in culture. However, the growth rate and the proliferative life span of the stem cells markedly increased using tissue culture dishes coated with a basement membrane-like extracellular matrix, which was produced by PYS-2 cells or primary endothelial cells. Furthermore, the stem cells expanded on the extracellular matrix, but not those on plastic tissue culture dishes, retained the osteogenic, chondrogenic, and adipogenic potential throughout many mitotic divisions. The extracellular matrix had greater effects on the proliferation of MSC and the maintenance of the multi-lineage differentiation potential than basic fibroblast growth factor. Mesenchymal stem cells expanded on the extracellular matrix should be useful for regeneration of large tissue defects and repeated cell therapies, which require a large number of stem or progenitor cells." @default.
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• W2063697785 date "2004-01-01" @default.
• W2063697785 modified "2023-10-15" @default.
• W2063697785 title "A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix" @default.
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• W2063697785 doi "https://doi.org/10.1016/j.bbrc.2003.11.143" @default.
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