FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063704292> ?p ?o ?g. }

• W2063704292 endingPage "446" @default.
• W2063704292 startingPage "437" @default.
• W2063704292 abstract "A 40 kDa glycoprotein (SPG-40) secreted during involution works as a protective signalling factor through its binding to viable cells. The crystal structure of the native protein has been determined at 2.3 A resolution. This is the first report on the carbohydrate-binding properties of SPG-40; the structure determinations of the complexes of SPG-40 with four oligosaccharides of different lengths at resolutions ranging from 2.2 to 2.8 A are described. Carbohydrate-binding studies with N-acetylglucosamines (GlcNAc(n), n = 3-6) using fluorescence spectroscopy revealed poor binding effects with GlcNAc(3) and GlcNAc(4), while GlcNAc(5) and GlcNAc(6) bound to SPG-40 with considerable strength; the dissociation constants (K(d)) were estimated to be 260 +/- 3 and 18 +/- 4 microM, respectively. SPG-40 was cocrystallized with GlcNAc(3), GlcNAc(4), GlcNAc(5) and GlcNAc(6). The overall structure of native SPG-40 was essentially similar to that reported previously at low resolution. The structures of its complexes with GlcNAc(3), GlcNAc(4), GlcNAc(5) and GlcNAc(6) revealed the positions of these oligosaccharides in the carbohydrate-binding groove and provided insights into the mechanism of binding of oligosaccharides to SPG-40, indicating that the preferred subsites in the carbohydrate-binding groove of SPG-40 were from -4 to -2. The structure of the protein remained unperturbed upon binding of GlcNAc(3) and GlcNAc(4), but the structure changed significantly upon binding of GlcNAc(5) and GlcNAc(6). Significant conformational variations were observed in the sugar-binding groove: Trp78 partially flipped out of the barrel in GlcNAc(5), while in the GlcNAc(6) complex a completely flipped-out Trp78 was observed along with several other conformational changes, including those of Asp186 and Arg242. Such changes upon binding to carbohydrates have not previously been observed in chitin-hydrolyzing chitinases and reflect less favourable binding of carbohydrates to SPG-40. As this appears to essentially be a binding protein, this loss of binding affinity might be compensated by other intermolecular interactions such as protein-protein interactions and also by the binding of its own glycan chain." @default.
• W2063704292 created "2016-06-24" @default.
• W2063704292 creator A5003774336 @default.
• W2063704292 creator A5003776760 @default.
• W2063704292 creator A5013373238 @default.
• W2063704292 creator A5033584482 @default.
• W2063704292 creator A5066029953 @default.
• W2063704292 creator A5083794797 @default.
• W2063704292 creator A5090329856 @default.
• W2063704292 date "2007-03-16" @default.
• W2063704292 modified "2023-10-16" @default.
• W2063704292 title "Carbohydrate-binding properties of goat secretory glycoprotein (SPG-40) and its functional implications: structures of the native glycoprotein and its four complexes with chitin-like oligosaccharides" @default.
• W2063704292 cites W1504533757 @default.
• W2063704292 cites W1539796472 @default.
• W2063704292 cites W1546896179 @default.
• W2063704292 cites W1568609804 @default.
• W2063704292 cites W1892136299 @default.
• W2063704292 cites W1935712031 @default.
• W2063704292 cites W1984808204 @default.
• W2063704292 cites W2000149921 @default.
• W2063704292 cites W2003176514 @default.
• W2063704292 cites W2013083986 @default.
• W2063704292 cites W2043670023 @default.
• W2063704292 cites W2059711876 @default.
• W2063704292 cites W2060851093 @default.
• W2063704292 cites W2061515984 @default.
• W2063704292 cites W2066182028 @default.
• W2063704292 cites W2080476827 @default.
• W2063704292 cites W2095237995 @default.
• W2063704292 cites W2112018994 @default.
• W2063704292 cites W2126444743 @default.
• W2063704292 cites W2137625026 @default.
• W2063704292 cites W2142485165 @default.
• W2063704292 cites W2148748115 @default.
• W2063704292 cites W2165246631 @default.
• W2063704292 cites W2170710018 @default.
• W2063704292 cites W285396975 @default.
• W2063704292 doi "https://doi.org/10.1107/s0907444907001631" @default.
• W2063704292 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17372347" @default.
• W2063704292 hasPublicationYear "2007" @default.
• W2063704292 type Work @default.
• W2063704292 sameAs 2063704292 @default.
• W2063704292 citedByCount "10" @default.
• W2063704292 countsByYear W20637042922013 @default.
• W2063704292 countsByYear W20637042922014 @default.
• W2063704292 countsByYear W20637042922015 @default.
• W2063704292 countsByYear W20637042922018 @default.
• W2063704292 countsByYear W20637042922021 @default.
• W2063704292 crossrefType "journal-article" @default.
• W2063704292 hasAuthorship W2063704292A5003774336 @default.
• W2063704292 hasAuthorship W2063704292A5003776760 @default.
• W2063704292 hasAuthorship W2063704292A5013373238 @default.
• W2063704292 hasAuthorship W2063704292A5033584482 @default.
• W2063704292 hasAuthorship W2063704292A5066029953 @default.
• W2063704292 hasAuthorship W2063704292A5083794797 @default.
• W2063704292 hasAuthorship W2063704292A5090329856 @default.
• W2063704292 hasConcept C107824862 @default.
• W2063704292 hasConcept C108625454 @default.
• W2063704292 hasConcept C181199279 @default.
• W2063704292 hasConcept C185592680 @default.
• W2063704292 hasConcept C2778815778 @default.
• W2063704292 hasConcept C2778977261 @default.
• W2063704292 hasConcept C55493867 @default.
• W2063704292 hasConcept C71240020 @default.
• W2063704292 hasConcept C96739231 @default.
• W2063704292 hasConceptScore W2063704292C107824862 @default.
• W2063704292 hasConceptScore W2063704292C108625454 @default.
• W2063704292 hasConceptScore W2063704292C181199279 @default.
• W2063704292 hasConceptScore W2063704292C185592680 @default.
• W2063704292 hasConceptScore W2063704292C2778815778 @default.
• W2063704292 hasConceptScore W2063704292C2778977261 @default.
• W2063704292 hasConceptScore W2063704292C55493867 @default.
• W2063704292 hasConceptScore W2063704292C71240020 @default.
• W2063704292 hasConceptScore W2063704292C96739231 @default.
• W2063704292 hasIssue "4" @default.
• W2063704292 hasLocation W20637042921 @default.
• W2063704292 hasLocation W20637042922 @default.
• W2063704292 hasOpenAccess W2063704292 @default.
• W2063704292 hasPrimaryLocation W20637042921 @default.
• W2063704292 hasRelatedWork W1499861477 @default.
• W2063704292 hasRelatedWork W1500427933 @default.
• W2063704292 hasRelatedWork W1578965502 @default.
• W2063704292 hasRelatedWork W2042621009 @default.
• W2063704292 hasRelatedWork W2044766053 @default.
• W2063704292 hasRelatedWork W2055754312 @default.
• W2063704292 hasRelatedWork W2064882758 @default.
• W2063704292 hasRelatedWork W2394731435 @default.
• W2063704292 hasRelatedWork W2412944889 @default.
• W2063704292 hasRelatedWork W4238713258 @default.
• W2063704292 hasVolume "63" @default.
• W2063704292 isParatext "false" @default.
• W2063704292 isRetracted "false" @default.
• W2063704292 magId "2063704292" @default.
• W2063704292 workType "article" @default.