FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063735329> ?p ?o ?g. }

• W2063735329 endingPage "e18422" @default.
• W2063735329 startingPage "e18422" @default.
• W2063735329 abstract "Cellular prion protein (PRNP) is a glycoprotein involved in the pathogenesis of transmissible spongiform encephalopathies (TSEs). Although the physiological function of PRNP is largely unknown, its key role in prion infection has been extensively documented. This study examines the functionality of PRNP during the course of embryoid body (EB) differentiation in mouse Prnp-null (KO) and WT embryonic stem cell (ESC) lines. The first feature observed was a new population of EBs that only appeared in the KO line after 5 days of differentiation. These EBs were characterized by their expression of several primordial germ cell (PGC) markers until Day 13. In a comparative mRNA expression analysis of genes playing an important developmental role during ESC differentiation to EBs, Prnp was found to participate in the transcription of a key pluripotency marker such as Nanog. A clear switching off of this gene on Day 5 was observed in the KO line as opposed to the WT line, in which maximum Prnp and Nanog mRNA levels appeared at this time. Using a specific antibody against PRNP to block PRNP pathways, reduced Nanog expression was confirmed in the WT line. In addition, antibody-mediated inhibition of ITGB5 (integrin αvβ5) in the KO line rescued the low expression of Nanog on Day 5, suggesting the regulation of Nanog transcription by Prnp via this Itgb5. mRNA expression analysis of the PRNP-related proteins PRND (Doppel) and SPRN (Shadoo), whose PRNP function is known to be redundant, revealed their incapacity to compensate for the absence of PRNP during early ESC differentiation. Our findings provide strong evidence for a relationship between Prnp and several key pluripotency genes and attribute Prnp a crucial role in regulating self-renewal/differentiation status of ESC, confirming the participation of PRNP during early embryogenesis." @default.
• W2063735329 created "2016-06-24" @default.
• W2063735329 creator A5070259023 @default.
• W2063735329 creator A5080216798 @default.
• W2063735329 creator A5083222784 @default.
• W2063735329 creator A5089199566 @default.
• W2063735329 date "2011-04-04" @default.
• W2063735329 modified "2023-09-25" @default.
• W2063735329 title "Prion Protein Expression Regulates Embryonic Stem Cell Pluripotency and Differentiation" @default.
• W2063735329 cites W1520668014 @default.
• W2063735329 cites W1546648987 @default.
• W2063735329 cites W1966758700 @default.
• W2063735329 cites W1969015262 @default.
• W2063735329 cites W1969758868 @default.
• W2063735329 cites W1969875668 @default.
• W2063735329 cites W1974365957 @default.
• W2063735329 cites W1975163937 @default.
• W2063735329 cites W1976133898 @default.
• W2063735329 cites W1976272429 @default.
• W2063735329 cites W1977736137 @default.
• W2063735329 cites W1978523429 @default.
• W2063735329 cites W1979112354 @default.
• W2063735329 cites W1979550663 @default.
• W2063735329 cites W1979695510 @default.
• W2063735329 cites W1986500594 @default.
• W2063735329 cites W1992380356 @default.
• W2063735329 cites W1993252465 @default.
• W2063735329 cites W1994965007 @default.
• W2063735329 cites W1994993245 @default.
• W2063735329 cites W1998904995 @default.
• W2063735329 cites W2003214571 @default.
• W2063735329 cites W2006491574 @default.
• W2063735329 cites W2006810449 @default.
• W2063735329 cites W2008004502 @default.
• W2063735329 cites W2010608530 @default.
• W2063735329 cites W2015396326 @default.
• W2063735329 cites W2015774470 @default.
• W2063735329 cites W2021366288 @default.
• W2063735329 cites W2032338659 @default.
• W2063735329 cites W2032860676 @default.
• W2063735329 cites W2038402718 @default.
• W2063735329 cites W2042673292 @default.
• W2063735329 cites W2060199954 @default.
• W2063735329 cites W2065873129 @default.
• W2063735329 cites W2071360562 @default.
• W2063735329 cites W2072052853 @default.
• W2063735329 cites W2076020654 @default.
• W2063735329 cites W2080514032 @default.
• W2063735329 cites W2092049899 @default.
• W2063735329 cites W2092220530 @default.
• W2063735329 cites W2093015386 @default.
• W2063735329 cites W2093562156 @default.
• W2063735329 cites W2096510701 @default.
• W2063735329 cites W2102252216 @default.
• W2063735329 cites W2117623697 @default.
• W2063735329 cites W2130547480 @default.
• W2063735329 cites W2138030809 @default.
• W2063735329 cites W2138712855 @default.
• W2063735329 cites W2147592113 @default.
• W2063735329 cites W2150163104 @default.
• W2063735329 cites W2150274419 @default.
• W2063735329 cites W2152131599 @default.
• W2063735329 cites W2158402820 @default.
• W2063735329 cites W2162594375 @default.
• W2063735329 cites W2162696534 @default.
• W2063735329 cites W2793841049 @default.
• W2063735329 cites W4255176992 @default.
• W2063735329 doi "https://doi.org/10.1371/journal.pone.0018422" @default.
• W2063735329 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3070729" @default.
• W2063735329 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21483752" @default.
• W2063735329 hasPublicationYear "2011" @default.
• W2063735329 type Work @default.
• W2063735329 sameAs 2063735329 @default.
• W2063735329 citedByCount "40" @default.
• W2063735329 countsByYear W20637353292012 @default.
• W2063735329 countsByYear W20637353292013 @default.
• W2063735329 countsByYear W20637353292014 @default.
• W2063735329 countsByYear W20637353292015 @default.
• W2063735329 countsByYear W20637353292016 @default.
• W2063735329 countsByYear W20637353292017 @default.
• W2063735329 countsByYear W20637353292019 @default.
• W2063735329 countsByYear W20637353292020 @default.
• W2063735329 countsByYear W20637353292021 @default.
• W2063735329 countsByYear W20637353292023 @default.
• W2063735329 crossrefType "journal-article" @default.
• W2063735329 hasAuthorship W2063735329A5070259023 @default.
• W2063735329 hasAuthorship W2063735329A5080216798 @default.
• W2063735329 hasAuthorship W2063735329A5083222784 @default.
• W2063735329 hasAuthorship W2063735329A5089199566 @default.
• W2063735329 hasBestOaLocation W20637353291 @default.
• W2063735329 hasConcept C104317684 @default.
• W2063735329 hasConcept C107459253 @default.
• W2063735329 hasConcept C143948831 @default.
• W2063735329 hasConcept C145103041 @default.
• W2063735329 hasConcept C148738053 @default.
• W2063735329 hasConcept C153911025 @default.
• W2063735329 hasConcept C180754005 @default.
• W2063735329 hasConcept C207701140 @default.

• next