Matches in SemOpenAlex for { <https://semopenalex.org/work/W2063760269> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2063760269 endingPage "54" @default.
- W2063760269 startingPage "49" @default.
- W2063760269 abstract "Kyasanur forest disease (KFD) is a zoonotic viral disease caused by infection by a Flavivirus, a member of the family Flaviviridae. KFD is a public health concern in the Karnataka State in southern India. Available conventional diagnostic tests such as virus isolation and serological tests, such as haemagglutination inhibition and complement fixation tests are time consuming. This study reports the development of a nested RT-PCR [nRT-PCR] and a TaqMan-based real-time RT-PCR and IgM antibodies capture ELISA [MAC-ELISA] for rapid and accurate diagnosis of suspected KFD cases. The nRT-PCR and the TaqMan-based real-time RT-PCR assays were developed using gene sequences of the NS-5/non-coding region. Both the assays detected KFD viral RNA in acute phase human serum samples and can provide early diagnosis of infection. Real-time RT-PCR was found to be more sensitive than nRT-PCR, which could detect 38 copies of KFDV RNA. MAC-ELISA was developed for the detection of recent infections. Although real-time RT-PCR and nRT-PCR require expensive reagents, expensive equipment and trained personnel, the developed MAC-ELISA can be used easily in the affected areas. These tests add to the existing diagnosis arsenal against haemorrhagic viruses that are prevalent in India. These assays will also help to extend our knowledge of the pathology of KFD virus and its associated clinical features, by measuring the viral titre during infection and at the time of seroconversion. Information, which is not available currently because of the lack of appropriate diagnostic methods. In addition, early laboratory diagnosis of KFDV infection will help in the application of appropriate control measures and management of KFD cases." @default.
- W2063760269 created "2016-06-24" @default.
- W2063760269 creator A5004869478 @default.
- W2063760269 creator A5009304672 @default.
- W2063760269 creator A5014829479 @default.
- W2063760269 creator A5019009128 @default.
- W2063760269 creator A5041595177 @default.
- W2063760269 creator A5042129289 @default.
- W2063760269 creator A5048239586 @default.
- W2063760269 creator A5058066928 @default.
- W2063760269 date "2012-12-01" @default.
- W2063760269 modified "2023-10-11" @default.
- W2063760269 title "Diagnosis of Kyasanur forest disease by nested RT-PCR, real-time RT-PCR and IgM capture ELISA" @default.
- W2063760269 cites W2148029044 @default.
- W2063760269 cites W2149981385 @default.
- W2063760269 doi "https://doi.org/10.1016/j.jviromet.2012.07.019" @default.
- W2063760269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22874757" @default.
- W2063760269 hasPublicationYear "2012" @default.
- W2063760269 type Work @default.
- W2063760269 sameAs 2063760269 @default.
- W2063760269 citedByCount "57" @default.
- W2063760269 countsByYear W20637602692012 @default.
- W2063760269 countsByYear W20637602692013 @default.
- W2063760269 countsByYear W20637602692014 @default.
- W2063760269 countsByYear W20637602692015 @default.
- W2063760269 countsByYear W20637602692016 @default.
- W2063760269 countsByYear W20637602692017 @default.
- W2063760269 countsByYear W20637602692018 @default.
- W2063760269 countsByYear W20637602692019 @default.
- W2063760269 countsByYear W20637602692020 @default.
- W2063760269 countsByYear W20637602692021 @default.
- W2063760269 countsByYear W20637602692022 @default.
- W2063760269 countsByYear W20637602692023 @default.
- W2063760269 crossrefType "journal-article" @default.
- W2063760269 hasAuthorship W2063760269A5004869478 @default.
- W2063760269 hasAuthorship W2063760269A5009304672 @default.
- W2063760269 hasAuthorship W2063760269A5014829479 @default.
- W2063760269 hasAuthorship W2063760269A5019009128 @default.
- W2063760269 hasAuthorship W2063760269A5041595177 @default.
- W2063760269 hasAuthorship W2063760269A5042129289 @default.
- W2063760269 hasAuthorship W2063760269A5048239586 @default.
- W2063760269 hasAuthorship W2063760269A5058066928 @default.
- W2063760269 hasConcept C104317684 @default.
- W2063760269 hasConcept C143660080 @default.
- W2063760269 hasConcept C159047783 @default.
- W2063760269 hasConcept C159654299 @default.
- W2063760269 hasConcept C203014093 @default.
- W2063760269 hasConcept C2522874641 @default.
- W2063760269 hasConcept C2777451964 @default.
- W2063760269 hasConcept C2778116200 @default.
- W2063760269 hasConcept C45189115 @default.
- W2063760269 hasConcept C48023723 @default.
- W2063760269 hasConcept C55493867 @default.
- W2063760269 hasConcept C86803240 @default.
- W2063760269 hasConceptScore W2063760269C104317684 @default.
- W2063760269 hasConceptScore W2063760269C143660080 @default.
- W2063760269 hasConceptScore W2063760269C159047783 @default.
- W2063760269 hasConceptScore W2063760269C159654299 @default.
- W2063760269 hasConceptScore W2063760269C203014093 @default.
- W2063760269 hasConceptScore W2063760269C2522874641 @default.
- W2063760269 hasConceptScore W2063760269C2777451964 @default.
- W2063760269 hasConceptScore W2063760269C2778116200 @default.
- W2063760269 hasConceptScore W2063760269C45189115 @default.
- W2063760269 hasConceptScore W2063760269C48023723 @default.
- W2063760269 hasConceptScore W2063760269C55493867 @default.
- W2063760269 hasConceptScore W2063760269C86803240 @default.
- W2063760269 hasIssue "1-2" @default.
- W2063760269 hasLocation W20637602691 @default.
- W2063760269 hasLocation W20637602692 @default.
- W2063760269 hasOpenAccess W2063760269 @default.
- W2063760269 hasPrimaryLocation W20637602691 @default.
- W2063760269 hasRelatedWork W1938450991 @default.
- W2063760269 hasRelatedWork W1986561264 @default.
- W2063760269 hasRelatedWork W2010455632 @default.
- W2063760269 hasRelatedWork W2063760269 @default.
- W2063760269 hasRelatedWork W2085137006 @default.
- W2063760269 hasRelatedWork W2364295079 @default.
- W2063760269 hasRelatedWork W2379475044 @default.
- W2063760269 hasRelatedWork W2403447631 @default.
- W2063760269 hasRelatedWork W3110097208 @default.
- W2063760269 hasRelatedWork W3140247098 @default.
- W2063760269 hasVolume "186" @default.
- W2063760269 isParatext "false" @default.
- W2063760269 isRetracted "false" @default.
- W2063760269 magId "2063760269" @default.
- W2063760269 workType "article" @default.